AKT2 antibody orb345005 from Biorbyt

Product Specs
ItemAKT2 antibody
CompanyBiorbyt
Price $3,787.00Supplier Page
Catalog Numberorb345005
Quantity1 mg
ApplicationsELISA, FC, IHC, WB
Concentration1.0 mg/ml
Conjugate/TagUnconjugated
FormatLiquid (sterile filtered)
PurityAnti-AKT2 antibody is directed against human AKT2. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT2 was purified from concentrated tissue culture supernate by Protein A chromatography. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 88% identity to mouse and 90% identity with rat, therefore, cross reactivity is expected
ReactivityHuman
TargetAKT2
IsotypeIgG2a
Clone16G11.E8
HostRat
ImmunogenAnti-AKT2 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein
NCBI Full Gene NameAKT serine/threonine kinase 2
NCBI Gene AliasesHIHGHH, PRKBB, RAC-BETA, PKBBETA, PKBB
ClonalityMonoclonal
StorageStore vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use

Specifications/Features

Application Notes: Anti-AKT2 Antibody is tested for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT2 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

Application Dilutions: ELISA: 1:2,000 - 1:10,000, FC: User Optimized, IHC: 20 µg/mL, WB: 1:500- 1:2000

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