IL-21 Antibody orb344650 from Biorbyt

Product Specs
ItemIL-21 Antibody
CompanyBiorbyt
Price $890.00Supplier Page
Catalog Numberorb344650
Quantity100 ug
ApplicationsELISA, WB
Conjugate/TagUnconjugated
Concentration1.0 mg/mL
FormatLyophilized
TargetIL-21
IsotypeIgG
HostRabbit
ReactivityBovine, Equine, Porcine
PurityThis product was Protein-A purified from monospecific antiserum by chromatography. This antibody is specific for bovine IL-21 protein. A BLAST analysis was used to suggest cross-reactivity with IL-21 from bovine sources based on 100% homology with the immunizing sequence. Based on 92% homology, there is a chance of cross-reactivity to porcine IL- 21, 91% to horse IL-21, 89% to human and dog, 88% to macaque, 84% to platypus and Syrian hamster, 76-79% to mouse, 75% to rat, and 48% to chicken. Cross-reactivity with IL-21 from other sources has not been determined
ImmunogenThis protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to amino acid residues 24-152 of bovine IL-21 protein
ClonalityPolyclonal
NCBI Full Gene Nameinterleukin 21
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use

Specifications/Features

Application Notes: IL-21 is expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes, and in HL-60 and THP-1 cell lines. This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15.1 kDa in size corresponding to bovine IL-21 protein by western blotting in the appropriate cell lysate or extract.

Application Dilutions: ELISA: 1:6,000, WB: 1 µg/mL

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