FITC Anti-GFP antibody ab6662 from Abcam

Product Specs
ItemFITC Anti-GFP antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab6662
Quantity100 µg
ApplicationsICC/IF, IHC-FoFr, IHC-Fr, WB
Conjugate/TagFITC
FormatGFP Fluorescein Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Concentration1 mg/ml
TargetGFP
HostGoat
IsotypeIgG
Gene NameGFP
Antibody TypePrimary Antibody
ClonalityPolyclonal
Research AreaResearch Tools

Questions and Answers

Can this antibody be used for immunofluorescence in mouse tissues?

Yes, the FITC Anti-GFP antibody has been successfully utilized for immunofluorescence in mouse tissues, as demonstrated in studies where it was applied to visualize specific proteins in various cellular contexts ().

What types of samples have been shown to work with this antibody in whole-mount immunofluorescence?

The antibody has been effectively used in whole-mount immunofluorescence to detect proteins in seminiferous tubules from neonatal mouse testes, indicating its applicability in developmental biology studies ().

Is this antibody suitable for use in histological applications?

Yes, the FITC Anti-GFP antibody has been employed in histological applications, including thin-section immunostaining, where it was used to visualize GFP-tagged proteins in tissue samples ().

What is the reactivity profile of this antibody across different species?

The FITC Anti-GFP antibody exhibits broad reactivity, being suitable for use in a wide range of species including human, mouse, rat, zebrafish, and various other vertebrates and invertebrates, making it versatile for comparative studies ().

Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Molecularly stratified hypothalamic astrocytes are cellular foci for obesityResearch SquareApril 24, 2026Tibor Harkany, Evgenii Tretiakov, Luis Varela, Jasna Jarc, Patrick Rebernik, Sylvia Newbold, Erik Keimpema, Alexei Verkhratsky, Tamas Horvath, Roman Romanovincubation (for 48h) with select combinations of primary antibodies: goat anti-GFP (1:1,000; Abcam, #ab6662, RRID:AB_305635: lot GR311622-15, GR311622-7), chicken anti-GFP (1:500, Aves Labs, #GFP-1020, RRID:AB_10000240:

(2) Hyperinnervation inhibits organ-level regeneration in mammalian skinCellMarch 20, 2026Hannah T. Tam, Jingyu Peng, Rebecca Freeman, Yulia Shwartz, Shlomi Brielle, Sakshi Garg, Siti Rahmayanti, Stephen J. Crocker, Devin Coon, Ya-Chieh Hsu(1:250),BioLegend,Cat#139313;RRID:AB_2563903 FACS-Ly6G-APC (1:100),BioLegend,Cat#127613;RRID:AB_1877163 Goat anti GFP (1:500),Abcam,Cat#ab6662;RRID:AB_305635 Goat anti Integrin alpha 8 (1:100),R&D Systems,Cat#AF4076;RRID:AB_2296280 Goat anti

(3) Simulated closed-loop magnetic stimulation promotes function recovery and axonal regeneration in spinal cord injuryCommunications BiologyMarch 18, 2026Lechi Zhang, Zhihang Xiao, Chunya Xia, Tingting Li, Zelin Su, Yingjie Fan, Xuyan Ren, Yaobo Liu, Min Suimmunostaining. The primary antibodies were diluted in BSA at the following concentrations: Go-GFP (Ab6662; Abcam, USA), 1:500; Mo-GFAP-cy3 (C9205, Merck, USA), 1:500; Rb-Neun-647 (62994, Cell Signaling Technology,

(4) Spatiotemporal targeting of messenger RNA lipid nanoparticles to the endometrium for the treatment of reproductive disordersNature NanotechnologyJanuary 19, 2026Saed Abbasi, Jairo Ortiz, Kimberly Bockley, Hongyu Feng, Emily Chen, Jordan Miller, Marina Better, Charles Eberhart, Neomi Jerry, Justin Hanes, et al.rabbit Alexa Fluor 647 anti-vimentin antibody (Abcam, EPR3776), goat FITC anti-GFP antibody (Abcam, Ab6662) and D-Luciferin sodium salt were purchased from Abcam. DAPI Fluoromount-G was obtained from SouthernBiotech. LNP

(5) Stress-induced sympathetic hyperactivation drives hair follicle necrosis to trigger autoimmunityCellJanuary 8, 2026Emily Scott-Solomon, Shlomi Brielle, Alexander O. Mann, Mark J. Khoury, Jingyu Peng, Liana Tellez, Mackenzie Harrigan, Myrto Ziogas, H. Amalia Pasolli, Monica Cassandras, et al.P-cadherin (1:200),R&D Systems,Catalog# AF761; RRID:AB_355581 Goat anti GFP-FITC (1:200),Abcam,"Catalog# ab6662, RRID:AB_305635" Rabbit anti c-Fos (1:1000),Abcam,Catalog# ab190289; RRID:AB_2737414 Rat anti CD3

(6) Enhanced proliferation tracer reveals Dorsal-Ventral asymmetry in tracheal epithelial RenewalStem Cell Research & TherapyJanuary 7, 2026Haiyuan Chen, Yazhu Zhong, Hao Zhang, Wei Yupreviously reported [17]. Primary antibodies used in this study were as follows: goat anti-GFP (Abcam, ab6662), rabbit anti-GFP (Invitrogen, A21311), rabbit anti-GSS (Proteintech, 11037-2-AP), goat anti-E-Cadherin

(7) Macrophage ITGAV is dispensable for post-infarction remodeling in mice and does not mediate fibronectin responsesCommunications BiologyNovember 29, 2025Ruoshui Li, Shuaibo Huang, Anis Hanna, Silvia C. Hernandez, Akihiko Kubota, Claudio Humeres, Izabela Tuleta, Nikolaos G. Frangogianniscourse of ITGAV activation in infarct macrophages, ITGAV (Abcam, ab179475) and GFP staining (Abcam, ab6662) was performed on sections from infarcted CSF1R-EGFP hearts at baseline, and after 24 h, 3 days, 7 days

(8) Cardiac mural cells are rate-limiting for coronary vascularization after heart injuryiScienceOctober 17, 2025Zhengkai Lu, Jian Zhou, Min Ye, Jufeng Meng, Teng Feng, Zhen Jiang, Wenxuan Dai, Defang Deng, Lei Yan, Yuanxin Wei, et al.REAGENT or RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, GFP,Abcam,Cat# ab6662; RRID: AB_305635 GFP,Nacalai Tesque,Cat# 04404-84; RRID: AB_10013361 GFP,Thermo Fisher Scientific,Cat#

(9) ETS transcription factor pointed controls germline survival in DrosophilaPLOS GeneticsAugust 25, 2025Alicia E. Rosales-Nieves, Miriam Marín-Menguiano, Lourdes López-Onieva, Juan Garrido-Maraver, Acaimo González-Reyesanti-cleaved-Caspase3 (from Cell Signaling Technology) 1:50; goat anti-GFP FITC-conjugated (Abcam Cat# ab6662, RRID:AB_305635) 1:500; rabbit anti-dp-ERK (Cell Signaling) 1:20; Rabbit anti-Caspase DCP1 (Cell Signaling)

(10) PKA restricts ERK signaling in learning and memory Kenyon cell neuronsCellular SignallingAugust 1, 2025James C. Sears, Kendal BroadieRT. For SPARK biosensor imaging, either native fluorescence or FITC-conjugated goat anti-GFP (Abcam ab6662; 1:500) labeling was used as indicated. After processing, brains were mounted between #1 coverslips,

(11) Highly dynamic mechanical transitions in embryonic cell populations during Drosophila gastrulationNat CommunJuly 14, 2025Juan Manuel Gomez, Carlo Bevilacqua, Abhisha Thayambath, Jean-Karim Heriche, Maria Leptin, Julio M. Belmonte, Robert PrevedelSigma), rabbit anti Snail17 1:500, rabbit anti GFP 1:1000 (ab290, Abcam), mouse anti FITC-GFP 1:250 (ab6662, Abcam), anti E-cadherin 1:50 (clone DCAD2, DSHB), anti Profilin/Chic (clone Chi-1J 1:50, DSHB), anti

(12) Non-canonical ALK7 pathways promote pancreatic cancer metastasis through β-catenin/MMP-mediated basement membrane breakdown and intravasationMolecular cancerJuly 4, 2025Kolarzyk AM, Kwon Y, Oh E, Lee KJ, Cho SY, Cano I, Lu R, Kwak TJ, Lee J, Wong G, et al.1:100), anti-CD31 (Dako, M0823, 1:200), and fluorescein isothiocyanate (FITC)-conjugated anti-GFP (Abcam, ab6662, 1:100) primary antibodies in blocking buffer overnight at 4 °C. The FITC-conjugated anti-GFP primary

(13) PADI4-mediated citrullination of histone H3 stimulates HIV-1 transcriptionNat CommunJune 25, 2025Luca Love, Bianca B. Jütte, Birgitta Lindqvist, Hannah Rohdjess, Oscar Kieri, Piotr Nowak, J. Peter Svenssonwith mounting medium with DAPI (Sigma-Aldrich, Cat# DUO82040), FITC-conjugated anti-GFP (Abcam, Cat#ab6662) was applied (1:1000) for 1 h at ambient temperature protected from light. Coverslips (VWR, Cat# MARI0117530)

(14) Engineering of CD63 Enables Selective Extracellular Vesicle Cargo Loading and Enhanced Payload DeliveryJ Extracell VesiclesJune 17, 2025Wataru Obuchi, Ayrton Zargani-Piccardi, Kevin Leandro, David Rufino-Ramos, Emilio Di Lanni, Dawn Madison Frederick, Katia Maalouf, Lisa Nieland, Tianhe Xiao, Pierre Repiton, et al.overnight at 4°C in blocking solution with primary antibodies: FITC anti‐GFP (Abcam, Cambridge, MA, ab6662, 1:200), rabbit anti‐RFP (Abcam, ab62341, 1:400) and Rat anti‐KI67 (Invitrogen, SolA15, 1:400). After

(15) Ki-67 promotes inflammatory signaling governing neutrophil recruitment during respiratory infectionsEMBO Molecular MedicineJune 10, 2025Min Yee, Ravi Misra, Sarah Vesecky, Michael Barravecchia, Rauf A Najar, Arshad Rahman, Gloria S Pryhuber, David A Dean, B Paige Lawrence, Daniel Fisher, et al.5,Invitrogen,MA5 -14473 Rat anti-Cytokeratin 8,DSHB,TROMA-1 CXCL5,Invitrogen,PA5-115069 Goat anti-EGFP,Abcam,Ab6662 Rabbit anti-Ki-67,Abcam,AB15580 Hamster anti-T1α/Podoplanin,DSHB,8.1.1 Rabbit anti-Phospho-Histone H3 (Ser10

(16) An Auxin Inducible Degradation System to Study Mklp2 Functions in MDCK Epithelial CellsBiol CellJune 9, 2025Morgane Rodriguez, Valérie Simon, Bénédicte Delaval, Benjamin VitreTransduction Laboratories #611082, IF: 1/250), Mklp2 (gift from A. Echard, WB: 1/2000), GFP‐FITC (Abcam #ab6662, IF: 1/400), GFP (Proteintech #66002, WB: 1/5000), GFP (Torrey Pines #401, WB: 1/5000), α‐tubulin (DM1α,

(17) Regulation of somatic stem cell and niche precursor fates and proliferation of by Wnt, JAK-STAT, Hedgehog and Hippo/Yorkie pathways during Drosophila pupal ovary development resembles the signaling framework organizing adult stem cell behaviorbioRxivMay 17, 2025Rachel Misner, Amy Reilein, Daniel Kalderonprimary antibodies used were anti-GFP (A6455, Molecular Probes) at 1:1,000; goat FITC-anti-GFP (Abcam ab6662) at 1:400. Secondary antibodies were Alexa-488, Alexa-546, Alexa-594 or Alexa-647 from Molecular Probes.

(18) N-cadherin facilitates trigeminal sensory neuron outgrowth and target tissue innervationDevelopmentMay 1, 2025Caroline A. Halmi, Carrie E. Leonard, Alec T. McIntosh, Lisa A. Taneyhillused included anti-Tubb3 (Abcam ab78078, 1:500 for section, 1:250 for whole-mount), anti-GFP (Abcam ab6662, 1:250), RFP (Invitrogen MA5-15257, 1:250), anti-TrkA (a gift from Dr Francis Lefcort, Montana State

(19) Neuron-restricted cytomegalovirus latency in the central nervous system regulated by CD4+ T-cells and IFN-γJ NeuroinflammationMarch 29, 2025Fran Krstanović, Andrea Mihalić, Ahmad Seyar Rashidi, Katarzyna M. Sitnik, Zsolt Ruzsics, Luka Čičin-Šain, Georges M. G. M. Verjans, Stipan Jonjić, Ilija Brizićautofluorescence. The following primary antibodies were used for IF: FITC goat anti-GFP (1:250, Abcam, #ab6662) and rabbit anti-NeuN (1:500, Abcam, #ab177487). Unconjugated primary antibodies were labeled with the

(20) Protocol for the in vivo tracing of alveolar type I cells from mice using two distinct dual recombinase-mediated genetic approachesSTAR ProtocolsMarch 21, 2025Xueying Yang, Xinfeng Meng, Jingting Zhu, Zhongxiao Wang, Bin Zhou, Kuo LiuRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Goat anti-GFP (dilution 1:500),Abcam,Cat#ab6662; RRID: AB_305635 Rabbit anti-GFP (dilution 1:500),Invitrogen,Cat#A11122; RRID:AB_2307355 "Rabbit anti-tdTomato

Be the First to Write a Review!

Molecular Therapy - Methods & Clinical Development

FITC Anti-GFP antibody from Abcam

Questions and Answers

Abcam

Citations (222)

Reviews

FITC Anti-GFP antibody from Abcam

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map