Fig 1: GFP expression in recellularized dermal constructs. An immunostaining protocol using a goat polyclonal anti-GFP antibody fluorescein isothiocyanate (FITC) (ab6662, Abcam, Littleton, CO) as the primary antibody was performed on fixed cryosectioned constructs. 4’, 6-Diamidino-2-phenylindole dihydrochloride (DAPI) nuclear stain was used to determine location of expression. (a–c) Intradermal injection of gWizGFP only control on DAPI (left) and FITC (center) channels, overlaid image in right column. (d–f) Constructs exposed to PAA for 3 minutes after intradermal injection of gWizGFP. n = 3. Scale bar = 50.0 μm. 200×.
Supplier Page from Abcam for Anti-GFP antibody (FITC)