SignalStain ® Boost IHC Detection Reagent (HRP, Mouse) 8125S from Cell Signaling Technology

Product Specs
ItemSignalStain ® Boost IHC Detection Reagent (HRP, Mouse)
CompanyCell Signaling Technology
Price $490.00 Inquire
Catalog Number8125S
Quantity15 ml
Conjugate/TagHRP
Research AreaRelated Products
ApplicationsIHC-p
Source/Expression SystemGoat
ReactivityM

Questions and Answers

Can this detection reagent be used for immunohistochemistry in mouse models?

Yes, the SignalStain® Boost IHC Detection Reagent (HRP, Mouse) has been utilized in immunohistochemical staining for mouse tissues, as demonstrated in various studies, including those involving tumor xenografts and other mouse models.

What is the recommended protocol for using this reagent in immunohistochemistry?

In a typical protocol, after fixing and sectioning the tissue, the slides should be incubated with the primary antibody, followed by the application of the SignalStain® Boost IHC Detection Reagent. The incubation time is generally around 30 to 45 minutes at room temperature, and the signal can be visualized using a DAB substrate kit.

Are there specific studies that have successfully employed this reagent in their experiments?

Yes, the reagent has been cited in multiple studies, including those investigating the role of circKL in kidney cancer and the effects of maternal diabetes on congenital heart disease, where it was used to detect specific proteins in tissue sections.

Does this reagent require any specific adjustments or titration for optimal results?

Some antibodies may require titration when used with this highly sensitive reagent to achieve optimal staining results, as indicated in the product description.

Cell Signaling Technology

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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Macrophage NEDD4L restrains liver fibrosis by preventing scar-associated macrophage expansion via ubiquitination of phospho-SMAD3Int J Biol SciMarch 17, 2026Yanghuan He, Shujun Ge, Shijia Ling, Siting Yang, Feiran Yang, Xinyi Liu, Siyue Dong, Yingfen Chen, Ziling Zhang, Yue Zhou, et al.°C and incubated with secondary antibodies (Cell Signaling Technology, Danvers, MA, catalog 8814S or 8125S) at room temperature for 1 hour. ImmPACT DAB Substrate Kit (Vector Laboratories, catalog ZK1018, CA,

(2) A ESRP1/circPHGDH/miR-149/RAP1B positive feedback loop promotes the malignant behaviors and glycolysis of prostate cancer cellExperimental & Molecular MedicineFebruary 27, 2026Xiang Wang, Limei Yu, Xiaoling Qian, Zhenfei Yuat 4 °C overnight, followed by incubation with an immunohistochemistry (IHC) detection reagent (no. 8125, Cell Signaling Technology) at 25 °C for 30 min. The results were visualized and captured under a light

(3) Iridophore apoptosis mediates socially-regulated developmental color pattern plasticity in an anemonefishPLOS BiologyFebruary 19, 2026Laurie J. Mitchell, Saori Miura, Youjung Han, Jann Zwahlen, Camille A. Sautereau, Bruno Frédérich, Vincent Laudetwere covered with 1–3 drops of SignalStain Boost Detection Reagent (Cell Signaling Technology Cat# 8125, RRID: AB_10547893) and incubated in a dark, humidified chamber for 30-mins at room temperature. Next,

(4) Selenoprotein H Functions as a PPARα Coactivator to Link Selenium Homeostasis to Hepatic Lipid Metabolism and Protect against SteatohepatitisAdvanced ScienceFebruary 8, 2026Yuwei Zhang, Yuchen Wang, Binbin Li, Xin Li, Chenyu Liu, Yanhao Chen, Cheng Tian, Dongmei Wang, Xiaosong Gu, Chunping Jiang, et al.buffer (pH 6.0) at 100°C. The HRP‐conjugated secondary antibody was used for signal detection (CST, 8125S). Immunohistochemistry‐stained tissue was visualized with Olympus IX73 microscopy. 5.14 Biochemical

(5) GSPT1-specific protein degradation is effective in preclinical models of chemoresistant MYCN-amplified neuroblastomaJournal of Experimental & Clinical Cancer ResearchFebruary 6, 2026Aleksandra Adamska, Hanna Chahin, Erick Andrés Muciño-Olmos, Javanshir Esfandyari, Kristina Aaltonen, Sofia Granados-Aparici, Joachim Tetteh Siaw, Katarzyna Radke, Chiara Lago, Paweł Pasikowski, et al.#MA5-32123). HRP-conjugated secondary antibody solutions (Cell Signaling; anti-rabbit: #8114S, anti-mouse: #8125S) were used for 1 h at RT for. ImmPACT DAB solution (Vector, #SK-4105) was applied for 5 min and nuclear

(6) KDM4A promotes NEPC progression through regulation of MYC expressionCancer LettersJanuary 28, 2026Celia Sze Ling Mak, Ming Zhu, Jie Fu, Xin Liang, Xiaoxuan Wang, Fei Yuan, Feng Wang, Anh G. Hoang, Xingzhi Song, Peter Shepherd, et al.ImmunoStar). Secondary antibodies used are as follows: anti-rabbit (RMR622L, Biocare), and anti-mouse (8125S, CST). The immunohistochemistry signals were developed with DAB Quanto (TA-125-QHDX, Epredia). The staining

(7) Targeting mTORC1 to promote ferroptosis and apoptosis in endometrial cancer with PI3K-Akt-mTOR pathway mutationFerroptosis and Oxidative StressDecember 29, 2025Yingying Hu, Pei Liu, Neal Rosen, Xuejun Jiangwith SignalStain® Boost IHC Detection Reagent (HRP) (Cell Signaling Technology 8114S for Rabbit or 8125P for Mouse) for 30 min. Slides were then stained with the SignalStain® DAB Substrate Kit (Cell Signaling Technology

(8) Integrated proteomic analysis identifies TYMS-dependent AMPK-mTOR signaling in pancreatic neuroendocrine tumorsiScienceNovember 21, 2025Vinod Vijayakurup, Benjamin Meyer, Syeda Rida Rizvi, Kara H. Kievit, Iram Hyder, Jin Koh, Michael Feely, Maria V. Guijarro, Frederic J. Kaye, Maria Zajac-Kayeantibody incubation using either the Vector Labs avidin-biotin ABC kit or Cell Signaling SignalStain (CST #8125) detection kit according to the manufacturer’s instructions. Secondary antibody or detection solution

(9) Modeling antithymocyte globulin-induced microvasculopathy using human iPSC-derived vascularized liver organoidsCell Reports MedicineNovember 6, 2025Shuntaro Kawamura, Yosuke Yoneyama, Norikazu Saiki, Yunheng Wu, Chiharu Moriya, Rio Ohmura, Mari Maezawa, Yoshihiro Shimada, Yicheng Wang, Kensaku Mori, et al.washed and treated with SignalStain Boost IHC Detection Reagent (HRP, Mouse; Cell Signaling Technology, 8125S). Color development was achieved using SignalStain DAB Substrate Kit (Cell Signaling Technology; 8059),

(10) Metastatic breast cancer cells are vulnerable to fatty acid oxidation inhibition through DDX3-DRP1-mediated mitochondrial plasticityRedox BiologyOctober 1, 2025Wen-Jing Hsu, Ming-Chien Hsu, Cheng-Ying Chu, Yu-Cheng Lee, Ching-Chieh Yang, Zei-Wei Liu, Chi-Ching Lee, Yang-Sen Lin, Cheng-Wei Linovernight at 4 °C. The next day, tissues were incubated with Signal Stain Boost IHC detection reagent (no. 8125 and no. 8114, Cell Signaling Technology) for 1 h at room temperature. Slides were washed with PBS, and

(11) Clinicopathological characteristics and treatment of patients with colorectal neuroendocrine carcinoma: A retrospective study of 157 casesOncology LettersSeptember 23, 2025Meng Dai, Shuang Sun, Xin Liu, Hulun LiCell Signaling Technology, Inc.) overnight at 4°C and with a HRP-conjugated anti-mouse secondary antibody (1:200; cat. no. 8125S; Cell Signaling Technology) for 30 min at room temperature; vii) color rendering using diaminobenzidine

(12) Clinicopathological characteristics and treatment of patients with colorectal neuroendocrine carcinoma: A retrospective study of 157 casesOncology LettersSeptember 23, 2025Meng Dai, Shuang Sun, Xin Liu, Hulun LiCell Signaling Technology, Inc.) overnight at 4°C and with a HRP-conjugated anti-mouse secondary antibody (1:200; cat. no. 8125S; Cell Signaling Technology) for 30 min at room temperature; vii) color rendering using diaminobenzidine

(13) Exercise duration and detraining influence not only body weight but also histopathological changes in the white adipose tissue of young male OLETF rats as an obesity modelPhysiol RepJuly 26, 2025Kanta Sakakura, Susumu Urakawa, Naoto FujitaSanta Cruz Biotechnology, CA, USA). Subsequently, the sections were exposed to HRP‐conjugated secondary antibody (#8125; Cell Signaling, MA, USA) for 30 min at room temperature and then visualized using DAB (#8059; Cell Signaling

(14) Rapamycin rescues APC-mutated colon organoid differentiationCancer Gene TherapyJuly 23, 2025Aline Habib, Rose Mamistvalov, Dalit Ben-Yosefwith PBST followed by 30 min of incubation with Boost Detection Reagent (HRP, Rabbit #8114 or Mouse #8125, Cell Signaling). The samples were then washed in water and PBS before incubation with the DAB Immunohistochemistry

(15) Role of mast cell-derived exosomes in exacerbating neuronal injury of experimental cerebral malariaParasit VectorsJuly 1, 2025Qianru Wang, Xiumei Mo, Hua Li, Mingqiu Ye, Guojun Fei, Pinru Chen, Yongfei Wang, Xinpeng Hou, Jiajing He, Wenbin Liu, et al.No. GB15138, Servicebio) at 4 °C overnight, then HRP-conjugated goat anti-mouse IgG (1:200; Cat. No. 8125P, Cell Signaling Technology) at 37 °C for 30 min. After PBS washes, sections were incubated overnight

(16) The roles of AKT isoforms in decidualization and embryo implantation using a Progesterone Receptor-Cre mouse model†Biology of ReproductionJune 15, 2025Pascal Adam, François Fabi, Sophie Parent, Léa-Isabelle Renaud, Monique Cadrin, Eric Asselinfollowing day, sections were incubated for 30 min with 25 μL SignalStain Boost Detection Reagent (#8125; Cell Signaling Technology) followed by an incubation in SignalStain DAB Substrate Kit (#8059; Cell Signaling Technology

(17) Downregulation of ubiquitous microRNA-320 in hepatocytes triggers RFX1-mediated FGF1 suppression to accelerate MASH progressionActa Pharmaceutica Sinica BJune 9, 2025Liu Yang, Wenjun Li, Yingfen Chen, Ru Ya, Shengying Qian, Li Liu, Yawen Hao, Qiuhong Zai, Peng Xiao, Seonghwan Hwang, et al.Afterward, the sections were washed with PBS and incubated with secondary antibodies (catalog No. 8814S or 8125S, Cell Signaling Technology [CST], Danvers, MA, USA) at room temperature for 40 min. After incubation,

(18) StarD5 Plays a Critical Role in the Hepatocyte ER Stress Survival ResponseInternational Journal of Molecular SciencesApril 27, 2025William M. Pandak, Genta Kakiyama, Daniel Rodriguez-AgudoFor the secondary antibody, SignalStain Boost IHC Detection reagent (HRP, Mouse) (Cell Signaling, Cat#8125, Danvers, MA, USA) was used as described by the manufacturer and then developed using a DAB substrate

(19) PGRMC2 is a pressure-volume regulator critical for myocardial responses to stress in miceNat CommunMarch 11, 2025Farideh Amirrad, Vivian La, Sharareh Ohadi, Miram Albotaif, Sha Webster, James K. Pru, Kiumars Shamloo, Ashraf M. Mohieldin, Surya M. Nauliwashing step and were incubated in the Signal Stain Boost Detection Reagent, HRP mouse (Cell Signaling, 8125) for 30 min at room temperature, followed by incubation in Signal Stain DAB (Cell Signaling, 8059) for

(20) Combined inhibition of focal adhesion kinase and RAF/MEK elicits synergistic inhibition of melanoma growth and reduces metastasesCell Rep MedFebruary 18, 2025Jared Almazan, Tursun Turapov, David A. Kircher, Karly A. Stanley, Katie Culver, A. Paulina Medellin, MiKaela N. Field, Gennie L. Parkman, Howard Colman, Silvia Coma, et al.,Cell Signaling Technology,Cat#8059 SignalStain Boost Detection Reagent,Cell Signaling Technology,Cat#8114 (Rabbit)Cat#8125 (Mouse) SignalStain Antibody Diluent,Cell Signaling Technology,Cat#8112 8-16% Tris-glycine polyacrylamide

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SignalStain ® Boost IHC Detection Reagent (HRP, Mouse)

SignalStain ® Boost IHC Detection Reagent (HRP, Mouse) from Cell Signaling Technology

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Cell Signaling Technology

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SignalStain ® Boost IHC Detection Reagent (HRP, Mouse) from Cell Signaling Technology

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