mTOR antibody orb345523 from Biorbyt

Product Specs
ItemmTOR antibody
CompanyBiorbyt
Price $784.00Supplier Page
Catalog Numberorb345523
Quantity100 µg
ApplicationsELISA, WB
Concentration0.9 mg/ml
Conjugate/TagUnconjugated
FormatLiquid (sterile filtered)
PurityThis is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs with both phosphorylated and non-phosphorylated forms of mTOR at S2448 from human derived tissues and cells. A BLAST analysis was used to suggest cross reactivity with mTOR protein from rat and mouse based on 100% homology with the immunizing sequence. Expect partial reactivity against mTOR homologues from zebrafish (94%) and dog (89%). Reactivity against homologues from other sources is not known
ReactivityHuman
TargetmTOR
IsotypeIgG
HostRabbit
ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 2430-2460 of human mTOR
NCBI Full Gene Namemechanistic target of rapamycin kinase
NCBI Gene AliasesRAFT1, SKS, FRAP1, FRAP2, RAPT1, FRAP
ClonalityPolyclonal
StorageStore vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use

Specifications/Features

Application Notes: This affinity purified mTOR antibody has been tested for use in ELISA and western blotting. ELISA data demonstrate reactivity against both phosphorylated and non-phosphorylated mTOR at S2448 and western blotting shows a band at approximately 250 kDa. Reactivity in other immunoassays is unknown.

Application Dilutions: ELISA: 1:50,000 - 1:100,000, WB: 1:250 - 1:2,000

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