GFP antibody orb345331 from Biorbyt

Product Specs
ItemGFP antibody
CompanyBiorbyt
Price $292.00Supplier Page
Catalog Numberorb345331
Quantity25 µl
ApplicationsDOT, ELISA, FC, IF, IHC, IP, WB
Concentration1.0 mg/ml
Conjugate/TagUnconjugated
FormatLiquid (sterile filtered)
PurityGFP Monoclonal Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215) from Aequorea victoria by both Western blot and ELISA. No reaction is seen against RFP
ReactivityOther
TargetGFP
IsotypeIgG
Clone9F9.F9
HostMouse
ImmunogenRecombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria
ClonalityMonoclonal
StorageStore vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing

Specifications/Features

Application Notes: Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. Tested in ELISA, IP, and WB and suitable in FACS, IHC, IF. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with Rockland's polyclonal anti-GFP (600-101-215) as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP (code # S000-03) or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

Application Dilutions: ELISA: 1:80,000 - 1:500,000, FC: User Optimized, IHC: 1:1,000 - 1:5,000, IF: User Optimized, WB: 1:300 - 1:30,000

Add to Compare List