IL-6 Antibody orb345128 from Biorbyt

Product Specs
ItemIL-6 Antibody
CompanyBiorbyt
Price $890.00Supplier Page
Catalog Numberorb345128
Quantity100 ug
ApplicationsELISA, IHC, WB
Conjugate/TagUnconjugated
Concentration1.0 mg/mL
FormatLyophilized
TargetIL-6
IsotypeIgG
HostRabbit
ReactivityHuman
PurityIL-6 antibody detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. The antiserum is also useful for neutralization of human of IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity. This product has minimal reactivity with mouse IL-6
ImmunogenThis purified antibody was prepared from whole rabbit serum produced by repeated immunizations with mature length recombinant human IL-6 produced in E.coli
NCBI Full Gene Nameinterleukin 6
NCBI Gene AliasesCDF, HSF, BSF-2, IL-6, IFNB2, HGF, BSF2, IFN-beta-2
ClonalityPolyclonal
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use

Specifications/Features

Application Notes: This purified antibody has been tested for use in IHC and western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be < 10 pg/µl by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Application Dilutions: ELISA: 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:300 - 1:1,000

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