L1/ORF2 antibody orb345069 from Biorbyt

Product Specs
ItemL1/ORF2 antibody
CompanyBiorbyt
Price $784.00Supplier Page
Catalog Numberorb345069
Quantity100 µg
ApplicationsDOT, ELISA, WB
Concentration1.0 mg/ml
Conjugate/TagUnconjugated
FormatLiquid (sterile filtered)
PurityL1/ORF2 Antibody is directed against two regions within the endonuclease domain of L1 ORF2 protein. This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. Reactivity occurs against human L1/ORF2 protein and is useful in determining its presence in various assays. A BLAST analysis was used to suggest cross reactivity with L1/ORF2 proteins from chimpanzee sources based on 100% homology with the immunizing sequences, and from macaque, fruit fly, cattle, dog, opossum, and rat sources based on 69 - 88% homology with the immunizing sequences
ReactivityHuman, Primate
TargetL1/ORF2
IsotypeIgY
HostGallus
ImmunogenThis IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein
NCBI Full Gene NameNinjurin A
NCBI Gene AliasesCG6449, Dmel\CG6449, nija, ninA
ClonalityPolyclonal
StorageStore vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use

Specifications/Features

Application Notes: Anti-L1/ORF2 Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 149 kDa in size corresponding to L1 protein by western blotting in the appropriate cell lysate or extract.

Application Dilutions: ELISA: 1:10,000, WB: 1:2,000-1_10,000

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