TNF Alpha antibody orb345256 from Biorbyt

Product Specs
ItemTNF Alpha antibody
CompanyBiorbyt
Price $292.00Supplier Page
Catalog Numberorb345256
Quantity25 µl
ApplicationsELISA, IHC, WB
Concentration1.15 mg/mL
Conjugate/TagUnconjugated
FormatLiquid (sterile filtered)
PurityThis product was Protein-A purified from monospecific antiserum by chromatography. It is specific for swine TNFα protein. A BLAST analysis was used to suggest cross-reactivity with TNFα from swine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against TNFα from baboon based on 90% homology. Cross-reactivity with TNFα from sources other than swine has not been determined
ReactivityPorcine
TargetTNF Alpha
IsotypeIgG
HostRabbit
ImmunogenThis protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to 154 amino acids of mature swine TNFα
NCBI Full Gene Nametumor necrosis factor
NCBI Gene AliasesTNFSF2, TNFa
ClonalityPolyclonal
StorageStore vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing

Specifications/Features

Application Notes: This protein A purified TNFα antibody has been tested by western blot and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 16.9 kDa in size corresponding to swine TNFα by western blotting in the appropriate cell lysate or extract.

Application Dilutions: ELISA: 1:2,000 - 1:2,500, IHC: User Optimized, WB: 1:1000

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