AKT3 PE Antibody orb344550 from Biorbyt

Product Specs
ItemAKT3 PE Antibody
CompanyBiorbyt
Price $920.00Supplier Page
Catalog Numberorb344550
Quantity50 ug
ApplicationsDOT, ELISA
Concentration1mg/ml
Conjugate/TagRPE
FormatLyophilized
PurityAnti-AKT3 antibody is directed against human AKT3. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT3 antibody was purified from tissue culture by Protein A chromatography. Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT has not been determined
ReactivityHuman, Mouse, Rat
TargetAKT3 PE
IsotypeIgG1
Clone25F6.F6.D8
HostMouse
ImmunogenAnti-AKT3 Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography using a synthetic peptide corresponding to internal residues of human AKT3 protein
NCBI Full Gene NameAKT serine/threonine kinase 3
NCBI Gene AliasesPKB-GAMMA, STK-2, PRKBG, RAC-gamma, MPPH2, MPPH, PKBG, RAC-PK-gamma
ClonalityMonoclonal
StorageStore vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis

Specifications/Features

Application Notes: Anti-AKT3 PE Antibody has been tested by ELISA and dot blot and is suitable for Flow Cytometry, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT3 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

Application Dilutions: ELISA: User Optimized, FC: User Optimized, IHC: User Optimized, IF: User Optimized, WB: User Optimized

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