Fig 1: Fbxl5 is required for the destruction of hSSB1. (A) A549 cells were transfected with a validated SMARTpoolTM siRNA library (Dharmacon) targeting all known human F-box genes. Cells were collected and cell lysate was analysis with western blot assay with hSSB1 antibody. (B) A549 cells were transfected with siRNAs for 48 h, targeting Beta-trcp1, Fbxl5 or Cullin1, respectively. The whole cell lysate was detected by western blot using indicated antibodies. (C) A549 cells were transfected with shRNAs targeting Fbxl5 for 36 h. The whole cell lysate was detected by western blot using indicated antibodies. (D) A549 cells were transfected with increase dose of Fbxl5 plasmids for 36 h. The whole cell lysate was detected by western blot using indicated antibodies.