GADD45 alpha (D17E8) Rabbit mAb 4632S from Cell Signaling Technology

Product Specs
ItemGADD45 alpha (D17E8) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number4632S
Quantity100 µl
ApplicationsW
ReactivityH, M, R, Mk
TargetGADD45A
HostRabbit
NCBI Gene AliasesDDIT1, GADD45
ClonalityMonoclonal
NCBI Full Gene Namegrowth arrest and DNA damage inducible alpha
Research AreaCell Cycle / Checkpoint Control
IsotypeIgG
CloneD17E8
Molecular Weight22

Questions and Answers

What applications has the GADD45 alpha antibody been used for in scientific research?

The GADD45 alpha antibody has been shown to work in Western Blot applications, as evidenced by its use in various studies to detect GADD45A protein levels in different experimental contexts, including cancer research and cellular response to stress.

Can this antibody be used to study the role of GADD45A in muscle denervation?

Yes, the GADD45 alpha antibody has been utilized in studies examining the role of GADD45A in muscle denervation, particularly in the context of signaling pathways such as PKB/Akt and mTORC1, which are crucial for muscle homeostasis and response to nerve injury ().

Is the GADD45 alpha antibody effective in detecting protein levels in human cancer cell lines?

The GADD45 alpha antibody has been effectively used to detect GADD45A protein levels in various human cancer cell lines, including those involved in T cell acute lymphoblastic leukemia, where it was used to assess the impact of treatments on cell viability and apoptosis ().

How does GADD45A influence cellular processes in the context of myocardial ischemia/reperfusion injury?

GADD45A plays a significant role in myocardial ischemia/reperfusion injury by mediating pathways such as JNK/p38 MAPK and STAT3/VEGF, which are involved in apoptosis and angiogenesis. The antibody has been used to assess GADD45A expression levels in studies investigating these mechanisms ().

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(1) ATGL sensitizes hepatocellular carcinoma cells to genotoxic drugs by modulating p53 acetylation/phosphorylation statusCell Death DiscoveryMarch 20, 2026Serena Castelli, Angela De Cristofaro, Enrico Desideri, Emanuele Salvi, Fabio Ciccarone, Maria Rosa Ciriolo(Cell Signaling Technology, cat. number #9718) or GADD45A (Cell Signaling Technology, cat. number #4632) antibodies and then incubated for 1 h with an Alexa Fluor™ 594 donkey anti-rabbit IgG (H + L) secondary

(2) CREB2 Functions as a Central Mediator of Oxidative Neuronal Death Triggered by Microglial Glutamate Release Under Neuroinflammatory ConditionsCellular and Molecular NeurobiologyFebruary 13, 2026Uk Yeol Moon, Young Eun Kim, Huu Dat Nguyen, Hye Joung ChoiAB_27113937), phospho-p53 (Ser15) (1:1000; CST, cat. no. 9284, RRID: AB_331464), GADD45α (1:1000; CST, cat. no. 4632, RRID: AB_10694428), GAPDH (1:1000; CST, cat. no. 5174, RRID: AB_10622025), and β-actin (1:200; Santa Cruz Biotechnology

(3) GADD45α is a direct target of TFEB and contributes to tacrolimus-induced chronic nephrotoxicityJCI InsightFebruary 6, 2025Ping Gao, Xinwei Cheng, Maochang Liu, Hui Peng, Guodong Li, Tianze Shang, Jianqiao Wang, Qianyan Gao, Chenglong Zhu, Zhenpeng Qiu, et al.incubation with anti–TGF-β (Santa Cruz Biotechnology, sc-130348), anti-GADD45α (Cell Signaling Technology, 4632S), or anti-γH2AX (Cell Signaling Technology, 9718T) overnight at 4°C. Then they were incubated with the

(4) Huyang Yangkun formula regulates the mitochondria pathway of ovarian granulosa cell apoptosis through FTO/m6A-P53 pathwayFrontiers in PharmacologyNovember 8, 2024Yang Li, Lingdi Wang, Jian Liu, Guangning Nie, Hongyan Yangdiluted at 1:1,000):ALKBH5, ab195377, Abcam; FTO, ab92821, Abcam; pJNK,4,668, CST; P38,8690, CST; PP38,4632, CST NRF2,16396-1-AP, Proteintech; BCL2,2876, CST; BCLXL, ab32370, Abcam; MCL1, ab32087, Abcam; BAX,

(5) The Influence of Aging on the Unfolded Protein Response in Human Skeletal Muscle at Rest and Following Acute ExerciseMedicine & Science in Sports & ExerciseNovember 1, 2024KELLY L. MICHIE, HAWLEY E. KUNZ, SURENDRA DASARI, IAN R. LANZA1:2000 (Invitrogen, MA1-91034); IRE1α 1:1000 (Cell Signaling, 14C10); GADD45α 1:500 (Cell Signaling, #4632); GADD34 1:1000 (Invitrogen, #PA1-139). All secondary antibodies were used at a concentration of 1:20,000,

(6) Chitotriose Enhanced Antitumor Activity of Doxorubicin through Egr1 Upregulation in MDA-MB-231 CellsMarine DrugsDecember 29, 2023Heng Li, Ke Ji, Peng Liu, Yan Geng, Jinsong Gong, Chao Zhang, Zhenzhong Ding, Zhenghong Xu, Jinsong Shinumber: 4154S), growth arrest, and the DNA damage-inducible protein alpha (GADD45A) (catalogue number: 4632S), and β-actin (catalogue number: 4970S) were procured from Cell Signaling Technology, Inc. (Danvers,

(7) Tension force causes cell cycle arrest at G2/M phase in osteocyte-like cell line MLO-Y4HeliyonFebruary 1, 2023Shimizu N, Fujiwara K, Mayahara K, Motoyoshi M, Takahashi T.anti-phospho-53 at Ser15 (#9248; Cell Signaling Technology, Danvers, MA, USA), and anti-GADD45A (D17E8, #4632; Cell Signaling Technology) antibodies at 4 °C. After 24-h incubation, membranes were washed in Tris-buffered

(8) Role of GADD45A in myocardial ischemia/reperfusion through mediation of the JNK/p38 MAPK and STAT3/VEGF pathwaysInternational journal of molecular medicineDecember 1, 2022Wang Y, Gao H, Cao X, Li Z, Kuang Y, Ji Y, Li Y.2 h and then incubated at 4°C overnight with primary antibodies against: GADD45A [1:1,000; cat. no. 4632; Cell Signaling Technology, Inc.(CST)], CD31 (1:1,000; cat. no. ab281583; Abcam), phosphorylated (p)-eNOS

(9) Transcriptomic analysis reveals crucial regulatory roles of immediate-early response genes and related signaling pathways in coronavirus infectious bronchitis virus infectionVirologyOctober 1, 2022Yuan LX, Yang B, Fung TS, Chen RA, Liu DX.and 100 g/ml streptomycin. Antibodies against cFOS (#2250), cJUN (#9165), SGK1 (#12103), GADD45A (#4632), β-actin (#4967) were purchased from Cell Signaling Technology. RGS2 (10,678-1AP) and RND3 (66228-1-Ig)

(10) Systematic lncRNA mapping to genome-wide co-essential modules uncovers cancer dependency on uncharacterized lncRNAseLifeJune 13, 2022Mitra R, Adams CM, Eischen CM.previously published (Eischen et al., 1999). Antibodies include CCNA2 (E1D9T, #91500), GADD45A (D17E8, #4632), CDK1 (POH1, #9116), and CDC25C (5H9, #4688) from Cell Signaling Technology; CDKN1A (F-5, sc-6246)

(11) ADRAM is an experience-dependent long noncoding RNA that drives fear extinction through a direct interaction with the chaperone protein 14-3-3Cell ReportsMarch 22, 2022Wei Wei et al.,Active motif,39685 Rabbit anti-CBP,Abcam,ab10489 Rabbit anti-S9.6,Merck,MABE1095 Rabbit anti-Gadd45a,Cell Signaling,4632 Rabbit anti-14-3-3 β/α,Cell Signaling,9636 Rabbit anti-NeuN-488,Abcam,Ab190195 Rabbit anti-Arc-647,Bioss,BS-0385R-A647 Rabbit

(12) Role of Klotho as a Modulator of Oxidative Stress Associated with Ovarian Tissue CryopreservationInternational Journal of Molecular SciencesDecember 17, 2021Boram Kim, Hyunho Yoon, Tak Kim, Sanghoon LeeCell Signaling Technology), SOD (1:1000, Cat#: 13141, Cell Signaling Technology), and GADD45a (1:1000, Cat#: 4632, Cell Signaling Technology). The membranes were incubated for 1 h with the following secondary antibodies:

(13) Inhibition of nonsense-mediated decay rescues p53β/γ isoform expression and activates the p53 pathway in MDM2-overexpressing and select p53-mutant cancersThe Journal of biological chemistryNovember 1, 2021Gudikote JP, Cascone T, Poteete A, Sitthideatphaiboon P, Wu Q, Morikawa N, Zhang F, Peng S, Tong P, Li L, et al.2527S), PUMA (D30C10, Catalog #12450S), p21 Waf1/Cip1 (DCS60, Catalog # 2946S), Gadd45a (Catalog # 4632S), Upf1 (Catalog # 9435), GAPDH (Catalog # 5174) from Cell Signaling Technology, MDM2 (Catalog # sc-56154)

(14) Arginine Methyltransferase PRMT1 Regulates p53 Activity in Breast CancerLife (Basel, Switzerland)August 5, 2021Liu LM, Tang Q, Hu X, Zhao JJ, Zhang Y, Ying GG, Zhang F.(PM020) was from MBL; antibody for FLAG (F3165) was from Sigma; antibodies for p21 (2947) and GADD45A (4632S) were from CST; and the antibody for HA (11867423001) was from Roche. 2.3. Plasmids Plasmids used

(15) LINC01116 promotes the proliferation and invasion of glioma by regulating the microRNA‑744‑5p‑MDM2‑p53 axisMolecular Medicine ReportsMarch 16, 2021Li Jiang, Chao Cheng, Wei Ji, Hao Wang, Quan Du, Xiaoqiao Dong, Junfei Shao, Wenhua Yuanti-MDM2 (1:1,000; cat. no. 86934), anti-BAK1 (1:1,000; cat. no. 12105), anti-GADD45A (1:1,000; cat. no. 4632), anti-β-actin (1:1,000; cat. no. 3700), anti-mouse IgG (1:5,000; cat. no. 7076) and anti-rabbit IgG

(16) Sinapic Acid Protects SH-SY5Y Human Neuroblastoma Cells against 6-Hydroxydopamine-Induced NeurotoxicityBiomedicinesMarch 13, 2021Tungalag T, Yang DK.(CHOP; #2895; 1:1000, cell signaling); growth arrest and DNA damage-inducible protein45α (GADD45α; #4632; 1:1000, Cell signaling); KDEL (ab12223; 1:1000, Abcam) for detecting glucose regulated protein (GRP)

(17) Aberrant methylation of GADD45A is associated with decreased radiosensitivity in cervical cancer through the PI3K/AKT signaling pathwayOncology LettersNovember 3, 2020Meng Lou, Rong Li, Ting-Yuan Lang, Li-Ying Zhang, Qi Zhou, Li Litemperature. The membranes were incubated with primary antibodies against GADD45A (1:1,000; cat. no. 4632), AKT (1:1,000; cat. no. 4685), p‑AKT (Ser 473) (1:2,000; cat. no. 4060) and GAPDH (1:1,000; cat. no.

(18) Linalool inhibits the growth of human T cell acute lymphoblastic leukemia cells with involvement of the MAPK signaling pathwayOncology lettersNovember 1, 2020Gong X, Wang B, Yan L, Lu X, Zhao X.p-ERK1/2 (1:1,000; cat. no. 4370), Growth Arrest And DNA Damage Inducible α (GADD45A) (1:1,000; cat. no. 4632S), caspase-3 (1:1,000; cat. no. 9662), cleaved caspase-3 (1:1,000; cat. no. 9664) and secondary antibodies

(19) IER5, a DNA damage response gene, is required for Notch-mediated induction of squamous cell differentiationeLifeSeptember 16, 2020Pan L, Lemieux ME, Thomas T, Rogers JM, Lipper CH, Lee W, Johnson C, Sholl LM, South AP, Marto JA, et al.,Cell Signaling Technology,Cat. #: 5276,"WB, 1:1000" Antibody,Rabbit monoclonal anti-GADD45A,Cell Signaling Technology,Cat. #: 4632,"WB, 1:1000" Antibody,Rabbit monoclonal anti-ID3,Cell Signaling Technology,Cat. #: 9837,"WB, 1:1000" Antibody,Horse

(20) Gambogenic acid exerts anticancer effects in cisplatin‑resistant non‑small cell lung cancer cellsMol Med RepJanuary 3, 2020Daofu Shen, Yu Wang, Hongmei Niu, Chunying Liuantibodies against p21 (1:1,000; cat. no. 2947; Cell Signaling Technology, Inc.), GADD45A (1:1,000; cat. no. 4632; Cell Signaling Technology, Inc.), cyclin D3 (1:2,000; cat. no. 2936; Cell Signaling Technology, Inc.),

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GADD45 alpha (D17E8) Rabbit mAb

GADD45 alpha (D17E8) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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GADD45 alpha (D17E8) Rabbit mAb from Cell Signaling Technology

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