Histone H3 (D1H2) XP ® Rabbit mAb 4499S from Cell Signaling Technology

Product Specs
ItemHistone H3 (D1H2) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $386.00 Inquire
Catalog Number4499S
ClonalityMonoclonal
NCBI Full Gene NameH3 clustered histone 1
ApplicationsWB, FCM, ICC, IF, IHC-p
ReactivityHu, Ms, Rt, Mk
NCBI Gene AliasesH3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A
HostRabbit
TargetH3
Quantity100 µl

Questions and Answers

What applications has the Histone H3 (D1H2) XP Rabbit mAb been used for in scientific research?

The Histone H3 (D1H2) XP Rabbit mAb has been shown to work in various applications including Western Blotting, Flow Cytometry, Immunocytochemistry, Immunofluorescence, and Immunohistochemistry. For instance, it was utilized in a study to analyze protein synthesis and cell growth in response to Thallium(I) treatment, highlighting its role in detecting histone modifications in cellular stress contexts ().

Can this antibody be used for studies involving human cells?

Yes, the Histone H3 (D1H2) XP Rabbit mAb is reactive to Homo sapiens, making it suitable for studies involving human cells. It has been cited in numerous publications, including those investigating the effects of histone modifications on gene expression and cellular responses in human cell lines ().

Is there any evidence of this antibody being used in studies related to cancer research?

Yes, the antibody has been referenced in cancer research, particularly in studies examining the role of histone modifications in tumor biology. For example, it was used to investigate the impact of H3 K27M mutations on Polycomb Repressive Complex 2 activity in pediatric gliomas, demonstrating its relevance in understanding oncogenic mechanisms ().

How does the antibody perform in Western Blotting applications?

The Histone H3 (D1H2) XP Rabbit mAb has been effectively used in Western Blotting, as evidenced by its application in various studies. For instance, it was employed to detect histone H3 levels in experiments assessing the effects of different treatments on cell proliferation and apoptosis, confirming its utility in analyzing histone protein expression (; ).

Cell Signaling Technology

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) L-2-hydroxyglutarate impairs neuronal differentiation through epigenetic activation of MYC expressionJournal of Clinical InvestigationMay 1, 2026Wen Gu, Xun Wang, Ashley Solmonson, Ling Cai, Yi Xiao, Alpaslan Tasdogan, Jordan Franklin, Yuannyu Zhang, Hua Zhang, Aundrea K. Westfall, et al.used 2 μg of histone extract. Histone H3 was used as a loading control (Cell Signaling Technology, 4499). DNA dot blot analysis. DNA dot blot analysis was performed using a Bio-Dot Microfiltration Apparatus

(2) Sphingosine-1-Phosphate Promotes FOS Activation in Osteosarcoma Under Tumor AcidosisActa Physiol (Oxf)April 26, 2026Nicolò Bozzini, Margherita Cortini, Alberto Righi, Agamemnon E. Grigoriadis, Michael Dack, Elizabeta Ilieva, Federica Torricelli, Veronica Manicardi, Nicola Baldini, Sofia Avnetvisualization. The primary antibodies and concentrations utilized were as follows: anti‐histone H3 1:50 (4499S, Cell Signaling Technology, Danvers, Massachusetts, USA); anti‐acetylated histone H3 1:50 (06–599, Millipore,

(3) QW-5-70 targets the colchicine site and demonstrates antitumor activity in P-gp-overexpressing cancer modelsMol Cancer TherApril 22, 2026Yang Xie, Ruida Hou, Najah Albadari, Hao Chen, Darcie J. Miller, Judith Quadrozzi Quadrozzi Gruntz, Michael L. Oldham, Mir Shahriar Kamal, Jianxiong Jiang, Zhongzhi Wu, et al.(CST, #9114, RRID: AB_2074652), phospho-Histone H3 (CST, #9701, RRID: AB_331535), HistoneH3 (CST, #4499, RRID: AB_10544537), phospho-Bcl-2 (CST, #2827, RRID: AB_659950), MDR1/ABCB1 (P-gp) (CST, #13342, RRID:

(4) Epstein-Barr virus orchestrates spatial reorganization and immunomodulation in the classic Hodgkin lymphoma tumor microenvironmentCell Reports MedicineApril 21, 2026Yao Yu Yeo, Huaying Qiu, Yunhao Bai, Bokai Zhu, Yuzhou Chang, Fabio Iannelli, Stephanie Pei Tung Yiu, Jason Yeung, Hendrik A. Michel, Yuchen Wang, et al.EDHu-1),Novus Bio,Clone ID: EDHu-1Cat #: NB110-40686 Histone H3 (Clone D1H2),CST,Clone ID: D1H2Cat #: 4499 CD45RO (Clone UCHL1),BioLegend,Clone ID: UCHL1Cat #: 304202 CD153 (CD30L) (Polyclonal),R&D,Clone ID:

(5) A longitudinal, multi-omic atlas reveals the emergence of a spatially organized immunosuppressive ecosystem in resistant melanomaCell Reports MedicineApril 21, 2026Shiyou Wei, Yulan Deng, Jinho Lee, Hongbin Lan, Zhenyu Yang, Marilyne Labrie, Courtney B. Betts, Hao Duan, Benjamin Tate, Joanna Pucilowska, et al.H3 (clone D1H2) + Mouse anti-Nucleolin (clone NM106) cocktail,Cell Signaling Technology|Abcam,Cat# 4499S; RRID:AB_10544537|Cat# AB215396; RRID:AB_3677433 Rabbit anti-PD-L1 (clone E1L3N),Cell Signaling Technology,Cat#

(6) Lysine Acetyltransferase 6A Drives M1 Macrophage Polarization Through Metabolic Reprogramming in Sepsis-Induced Acute Lung InjuryBiomoleculesApril 20, 2026Xin Wang, Junlin Chen, Yimei Lai, Yumeng Wang, Kaixia Hu, Mengshi Wu, Niansheng Yang, Yuefang Huang(1:1000, Cell Signaling Technology, Cat# 8173T), Histone H3 (1:2000, Cell Signaling Technology, Cat# 4499T) and β-actin (1:1000, Servicebio, Wuhan, China, Cat# GB15003). Membranes were washed with TBST, incubated

(7) Interaction of NDRG1 and MRE11 Modulates DNA Replication and RepairCancers (Basel)April 20, 2026Hanna M. Doh, Nina Kozlova, Zhipeng A. Wang, Hwan Bae, Philip A. Cole, Taru Muranen(#4895, CST), Vinculin (#13901, CST), Myc (#2276, CST), Tubulin (#ab7291, Abcam, Waltham, MA, USA), H3 (#4499, CST), Cyclin A2 (#), pCDK2 (#2561, CST) Appropriate secondary antibodies (peroxidase-conjugated IgG,

(8) Spermidine Attenuates Neuroimmune Dysfunction in Gulf War Illness via Modulation of the Gut- Brain AxisMol NeurobiolApril 10, 2026Ayushi Trivedi, Subhajit Roy, Madhura More, Dipro Bose, Punnag Saha, Ram Kumar, Subham Sarkar, Jonathan Skupsky, Ashok Tuteja, Kimberly Sullivan, et al.(1:800; 12721S), anti-HO-1 (1:1000; 43966S), anti-β actin (1:2500; 4970S), and anti-Histone H3 (1:1500; 4499S), sourced from Cell Signaling Technology [CST] (Danvers, MA, USA). Species-appropriate HRP-conjugated

(9) HDAC Inhibition Induces Transient Phenotypic Inertia in Dormant OCCC Spheroids by Derepression of Cell Cycle GenesCellsApril 10, 2026Sylvia Cheng, Bart Kolendowski, Yudith Ramos-Valdes, Trevor G. Shepherd, Gabriel E. DiMattia,Cell Signaling Technology,9725 H3K4me3,Cell Signaling Technology,9751 Histone H3,Cell Signaling Technology,4499 Ki67,"Abcam (Cambridge, UK)",ab16667 p-Histone H3 (Ser28),Cell Signaling Technology,9701 Antibodies

(10) Cullin-3 adaptor SHKBP1 inhibits SQSTM1/p62 oligomerization and Keap1 sequestrationJ Cell BiolApril 6, 2026Lin Luan, Xiaofu Cao, Zijun Xia, Shivanshi Vaid, Manuel D. Leonetti, Jeremy M. BaskinCell Signaling Technology (2128S, RRID:AB_823664); rabbit anti-Histone H3, Cell Signaling Technology (4499S, RRID:AB_10544537); mouse anti-Nrf2 (A-10), Santa Cruz Biotechnology (sc-365949, RRID:AB_ 10917561).

(11) miR-369-3p Modulates LRRK2-Mediated Inflammation and Autophagy in RAW264.7 MacrophagesInt J Mol SciApril 2, 2026Viviana Scalavino, Emanuele Piccinno, Ilaria Grassi, Raffaele Armentano, Gianluigi Giannelli, Grazia Serino1:2000), Vinculin (#13901, Cell Signaling, Technology, Danvers, MA, USA, dilution 1:5000), Histone H3 (#4499, Cell Signaling, Technology, Danvers, MA, USA, dilution 1:1000), and secondary antibody goat anti-rabbit

(12) Targeting HIF-1α promotes ferroptosis and boosts antitumor immunity in MSS colorectal cancerRedox BiolApril 1, 2026Zhiying Yang, Rui Ma, Weili Wu, Ying Shi, You Chen, Xiaotong Luo, Kai Li, Liangcai Wu, Bo Wang, Boyu Zhang, et al.1:1000), α-tubulin (Proteintech, cat# 66031-1-Ig, 1:1000), Histone 3 (Cell Signaling Technology, cat# 4499s, 1:1000), 4-HNE (Abcam, cat# ab46545, 1:1000). 2.5 DNA constructs, transfection, and lentivirus production HIF-1α

(13) FNIP1 Modulates B Cell Receptor Signaling Strength by Coordinating Metabolism During DevelopmentbioRxivApril 1, 2026Heon Park, Ryan Culbert, Dechen Sakya, Raynah R. Silprasert, Brian M. IritaniRag and LAMTOR antibody sampler kit (RagA (4357), RagB (8150), RagC (9480), RagD (4470)), Histone H3 (4499P), TFE3 (14779s), TFEB (A303-673A, bethyl lab), α-rabbit IgG HRP (W4011, promega), α-mouse IgG HRP (W4021,

(14) OSBPL3 promotes LUAD metastasis and glycolysis through transcriptional upregulation of PLAU via NFE2L2Translational OncologyApril 1, 2026Qingqiong Zhang, Jingshun Zhang, Junxian Li, Shuang Chen, Yijun Liu, Kaidi Li, Minzhang GuoProteintech), β-actin (66,009-1-Ig, Proteintech), cleaved caspase-3 (25,128-1-AP, Proteintech), and Histone H3 (4499S, CST). Celigo-Based proliferation analysis Cell proliferation was assessed with a Celigo full-image

(15) The novel tertiary amine LSD1 inhibitor 596 inhibits endometrial cancer through the mTOR signal transduction pathwayTranslational OncologyApril 1, 2026Chunli Wang, Dandan Shen, Nanshan Lin, Panjie Wang, Yaping Bi, Huina Hu, Yujia Shang, Chenchen Ren, Li Yang, Qisheng Mamain purchasing information was listed below:LSD1 (1:10,000, ab129195, Abcam, UK), H3(1:20,000, No.4499T,Cell Signaling Technology, CST, Boston, MA, USA), H3K4me1(1:2000, No.5326T, CST), H3K4me2(1:2000, No.9725S,

(16) Interaction of NDRG1 and TGM2 modulates DNA replication and repairMolecular Cancer ResearchMarch 31, 2026Hanna M. Doh, Nina Kozlova, Kayla A. Cruz, Taru Muranen(#2276, CST), Tubulin (#ab7291, Abcam), HA (#2367, CST), GST (#sc-138, Santa Cruz Biotechnology), H3 (#4499, CST), pATR-S1989 (#58014, CST), pATR-S428 (#2853, CST), ATR (#2790, CST), pChk1-S317 (#12302, CST),

(17) Chiral methionine oxidation reagents reveal stereospecific proteome modificationsbioRxivMarch 30, 2026Angel Gonzalez-Valero, Annika C. S. Page, Jayde M. Bertoch, Fadi Alsarhan, Jaehee Kim, Amr A. Alazali, Ritwik R. Srinivas, Xiao Xie, Audrey G. Reeves, Kacper Skakuj, et al.3501), rabbit anti-RCAS1 (CST; 12290), rabbit anti-GAPDH (CST; 5714), and rabbit anti-Histone H3 (CST; 4499). All antibodies were diluted at 1:1000 in 5% BSA/TBST at 4 °C overnight. The next morning the blots

(18) A critical signaling role for diacylglycerol in phagocytosis of M. tuberculosisbioRxivMarch 26, 2026Alec Griffith, Michelle Garcia, Gaelen Guzman, Fikadu G. Tafesse(1:1000; Cell Signaling Technologies, product 4292S), Histone H3 (Cell Signaling Technologies, product 4499T), B-Tubulin (Cell Signaling Technologies, product 2128S), anti-rabbit IgG HRP-linked (Cell Signaling Technologies

(19) CircAFF3 modulation of p53–ID2 signaling in the retinal pigment epithelium links inflammation with cell death in dry age-related macular degenerationFrontiers in Cell and Developmental BiologyMarch 25, 2026Yeongseo Ryu, Dahee Jeong, Jeong Hun Kim, Young-Kook Kim2772s), p53 (Santa Cruz, sc-126), MDM2 (Santa Cruz, sc-13161), Histone H3 (Cell Signaling Technology, 4499s), Lamin B1 (Abcam, ab16048), and GAPDH (Santa Cruz, sc-32233). The following day, each membrane was

(20) Lipocalin 2 orchestrates resistance to ferroptosis via AXLCell ReportsMarch 24, 2026Sabrina Z. Wang, J. Payton Timken, Ellen S. Hong, Sehaj Kaur, Eli Newby, Kristen E. Kay, Erin E. Mulkearns-Hubert, Daniel J. Silver, Juyeun Lee, Joshua B. Rubin, et al.Tubulin,Promega,Cat #G7121; RRID:AB_430874 Rabbit anti-Phosphorylated Histone H3,Cell Signaling Technology,Cat #4499; RRID:AB_10544537 Rabbit anti-Cleaved Caspase 3,Cell Signaling Technology,Cat #9661; RRID:AB_2341188 Bacterial

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Histone H3 (D1H2) XP ® Rabbit mAb

Histone H3 (D1H2) XP ® Rabbit mAb from Cell Signaling Technology

Questions and Answers

Cell Signaling Technology

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Histone H3 (D1H2) XP ® Rabbit mAb from Cell Signaling Technology

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