RFP Antibody Peroxidase Conjugated Pre-Adsorbed orb345852 from Biorbyt

Product Specs
ItemRFP Antibody Peroxidase Conjugated Pre-Adsorbed
CompanyBiorbyt
Price $850.00Supplier Page
Catalog Numberorb345852
Quantity100 ug
ApplicationsELISA, IHC, WB
Concentration1.0 mg/mL
Conjugate/TagHRP
FormatLyophilized
PurityRabbit Anti-RFP Peroxidase Conjugated Antibody Pre-adsorbed was prepared from monospecific rabbit antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal
TargetRFP
IsotypeIgG
HostRabbit
ImmunogenThe immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma
ClonalityPolyclonal
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use

Specifications/Features

Application Notes: Anti-RFP can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP has been tested by ELISA and western blot. This product is used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

Application Dilutions: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,000, WB: 1:2,000 - 1:5,000

Add to Compare List