TNF Alpha antibody orb345125 from Biorbyt

Product Specs
ItemTNF Alpha antibody
CompanyBiorbyt
Price $1,805.00Supplier Page
Catalog Numberorb345125
Quantity1 mg
ApplicationsELISA, IF, IHC, WB
Concentration1.0 mg/mL
Conjugate/TagUnconjugated
FormatLiquid (sterile filtered)
PurityThis antibody is primarily directed against mature 17,000 MW human TNFα and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFα in the same formats using similar dilutions. The antibody does not recognize human TNFß (lymphotoxin). This IgG fraction antibody will recognize the cell-bound precursor of TNFα as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFα activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended
ReactivityHuman
TargetTNF Alpha
IsotypeIgG
HostRabbit
ImmunogenTNF alpha antibody used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human TNFα
NCBI Full Gene Namecarnitine palmitoyltransferase 1B, tumor necrosis factor
NCBI Gene AliasesCPT1-M, TNLG1F, DIF, CPT1M, CPTI, M-CPT1, TNFA, CPTI-M, TNFSF2, TNF-alpha, MCPT1, MCCPT1
ClonalityPolyclonal
StorageStore anti-TNF alpha at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use

Specifications/Features

Application Notes: Anti-TNF alpha antibody has been tested in WB, ELISA, IF, and IHC. Expect a band in appropriate lysates at ~25.6kDa. This antibody is also useful for neutralization of human and primate TNFα activity in bioassays diluted at 1:200. The antibody does not recognize human TNF beta (lymphotoxin). Specific conditions for reactivity and signal detection should be optimized by the end user.

Application Dilutions: ELISA: 1:1,000 - 1:5,000, IHC: 1:100 - 1:500, IF: User Optimized, WB: 1:500 - 1:2,000

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