Western blot analysis of PDX1 (arrow) using PDX1 Antibody (T11) (Cat.#TA302073). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDX1 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Phospho-PDX1-T11.ctrl antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

IF image of SY5Y cells stained with PDX1 (T11) antibody. SY5Y cells were incubated with TA302073 PDX1 (T11) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue). Note the highly specific localization of the PDX1 immunosignal to the nucleus, supported by Human Protein Atlas Data