Western blot analysis of RelB was performed by loading 20 ug of THP-1 (lane1), Daudi (lane2) and Raji (lane3) cell lysate on a gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skimmed milk for 1 hour at room temperature. RelB was detected at ~ 63 kDa using Mouse anti RelB (MCA6037) at 1-2 ug/ml in 5 % skimmed milk at 4°C overnight on a rocking platform. A goat anti-mouse HRP conjugated secondary antibody was used for detection

Western blot analysis of RelB was performed by loading 20 ug of THP-1 (lane1), Daudi (lane2) and Raji (lane3) cell lysate on a gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skimmed milk for 1 hour at room temperature. RelB was detected at ~ 63 kDa using Mouse anti RelB (MCA6037) at 1-2 ug/ml in 5 % skimmed milk at 4°C overnight on a rocking platform. A goat anti-mouse HRP conjugated secondary antibody was used for detection