Simple Western: xCT Antibody [TA301518] - Simple Western lane view shows a specific band for xCT using TA301518 at 25 ug/ml in HeLa and HeLa + DEM cell lysates. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Flow Cytometry: xCT Antibody [TA301518] - An intracellular stain was performed on HeLa cells with TA301518AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Simple Western: xCT Antibody [TA301518] - Simple Western lane view shows a specific band for xCT using TA301518 at 25 ug/mL in HepG2 cell lysates and antibody at 25 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 63 kDa. Image reported by internal validation.