Fig 2: Comparison of normalization efficiency on five H3K27ac data sets of adrenocortical carcinoma. Average density signal is shown for 8 kb regions surrounding TSSs of “constant genes”. The first plot corresponds to the average signal before the normalization. Antibody: ab4729 (rabbit polyclonal, Abcam)

Fig 3: Graphs generated by CHIPIN allowing evaluating specificity of two antibodies used: A antibody ab4729 (Abcam) against H3K27ac in an adrenocortical carcinoma sample (unpublished), and B antibody ACM39155 (Active Motif) against H3K27me3 in the shSpi1-A2B cells (unpublished). For ab4729, as expected, highly expressed genes show a higher level of H3K27ac than genes of medium or low expression; while for ACM39155, highly expressed genes show an increase of intensities for H3K27me3 downstream TSS (red). This suggests a potential non-specific binding of this antibody targeting H3K27 methylation to acetylated lysines, also documented by Rothbart et al. [20]