Fig 1: Protein expression of TMEindex genes in osteosarcoma tissues and their effects on proliferation, invasion and migration of osteosarcoma cells. A IHC staining images of MYC, P4HA1 and RAMP1 in osteosarcoma tissues and corresponding normal tissues. The IHC scores indicated that the protein expression of MYC, P4HA1 and RAMP1 was higher in tumor tissues. B Kaplan–Meier curves depict the RFS difference between high and low TMEindex proteins (MYC, P4HA1 and RAMP1) groups (all log-rank P < 0.0001) in the external cohort. Red representing the high TMEindex proteins group and blue representing the low TMEindex proteins group. C Folded line plots showing the effect of MYC, P4HA1, RAMP1 and TAC4 knockdown on the proliferation of MG-63 and U2OS cells. The blue line represents the control group and the red line represents the knockdown group. D Transwell chamber experiment showing the effect of MYC, P4HA1, RAMP1 and TAC4 knockdown on the invasion of MG-63 and U2OS cells. Scale bar: 100 µm. E Scratch assay showing the effect of MYC, P4HA1, RAMP1 and TAC4 knockdown on the migration of MG-63 and U2OS cells. Data are represented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001