Western blot analysis of RAPGEF2 in various lysates. Samples were incubated with RAPGEF2 polyclonal antibody (Product # PA5-49456) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: Hela whole cell lysates; Lane 2: HT-1080 whole cell lysates; Lane 3: 293T/17 whole cell lysates. Predicted band size: 167 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.