Fig 1: Molecular events for tumour cell repopulation correlate with poor clinical outcome. (A) The levels of X‐box binding protein‐1 (XBP1), amphiregulin (AREG) and basic fibroblast growth factor (bFGF) in representative cancer specimens were examined by immunohistochemistry (IHC) assays. Scale bar = 50 µm. (B) The correlation between the expression levels of XBP1s and AREG or bFGF was analysed. (C) The average IHC values of each protein (XBP1s, AREG, bFGF) in 88 tongue cancer tissues were obtained, and IHC scores above the average value indicated high expression, otherwise indicated low expression. Kaplan–Meier analysis indicated a correlation between XBP1s/AREG/bFGF levels and overall survival in patients with tongue cancer. Kaplan–Meier survival analysis is shown here with a two‐sided log‐rank p‐value. (D) The protein levels of AREG and bFGF in serum were examined by enzyme‐linked immunosorbent assay (ELISA) (n = 3), ** p < .01, *** p < .001. Data are presented as the mean value ± SEM. Statistical significance was determined by a two‐tailed Student's t‐test. (E) The correlation between the XBP1s/AREG/bFGF levels and tongue cancer recurrence was analysed. (F) Fifteen paired tissues of primary tongue cancer and recurrent tongue cancer were collected. The protein levels of p65, IκBα and lysine acetyltransferase 6B (KAT6B) were examined by IHC assays. The transcript levels of miR‐22‐3p, XLOC_003973 and XLOC_010383 were examined by in situ hybridisation (ISH) assays. Scale bars = 50 µm
Fig 2: A 32-yr-old female patient has a papillary carcinoma at the left lobe of the thyroid gland. A is an ultrasonogram, which reveals that the nodule does not invade the capsule, the size is approximately 1.5 × 0.9 cm. B is the pathology (H& E, × 100), which reveals that focal calcification can be observed. C is bFGF × 100, the cytoplasm is strongly positive (+++) in papillary thyroid carcinomas and negative in the surrounding normal thyroid tissue.
Fig 3: 27-HC was essential for cholesterol-induced the release of FGF-2 and IL-6.Cells were cultured in the monoculture or coculture system exposed to 1 μM 27-HC or 0.8 mg/ml cholesterol for 72 h. The supernatants were collected for cytokines analysis using Milliplex Map Multiple cytokines detection kit (A) by Luminex 200 and ELISA assay (B, C). *P < 0.05 vs. Con group; **P < 0.01 vs. Con group; ***P < 0.001 vs. Con group; #P < 0.05 vs. Sh-Ctrl group; ##P < 0.01 vs. Sh-Ctrl group; ###P < 0.001 vs. Sh-Ctrl group; &P < 0.05 vs. the corresponding group in the monoculture system; &&P < 0.01 vs. the corresponding group in the monoculture system; &&&P < 0.001 vs. the corresponding group in the monoculture system.
Fig 4: Angiogenesis, BBB repair, and antiapoptotic evaluation of EBP-bFGF/ECM-mediated repair in rats with cerebral ischemia. (A) Immunofluorescence staining of capillaries in the ischemic brain. Scale bars, 50 μm. Blue, DAPI; red, vWF. (B) Immunofluorescence staining of vessels in the ischemic brain. Scale bars, 50 μm. Blue, DAPI; green, α-SMA. (C) Immunofluorescence staining of tight junction proteins in the BBB in the ischemic brain. Scale bars, 20 μm (above) and 2 μm (below). Blue, DAPI; red, ZO-1. (D) TUNEL staining of apoptotic cells in the ischemic brain. Scale bars, 50 μm. Blue, DAPI; green, TUNEL. (E) Statistical analysis of capillaries. n = 6. (F) Statistical analysis of α-SMA+ vessels. n = 6. (G) Statistical analysis of mean fluorescence intensity of ZO-1. n = 6. (H) Statistical analysis of TUNEL+ cells. n = 5. Data represent means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.
Fig 5: Behavioral test of EBP-bFGF/ECM-mediated repair in rats with cerebral ischemia. (A) Rotarod test on the rotarod apparatus. Statistical analysis of the speed during the rotarod test. n = 6. (B) Rotarod test. Statistical analysis of the time during the rotarod test. n = 6. (C) The track of the rats during the open-field test was recorded by the SMART 3.0 system. (D) Statistical analysis of the total distance. n = 6. (E) The grip strength test by the BIO-GS3 instrument. Statistical analysis of grip strength test. n = 6. Data represent means ± SD, *P < 0.05, **P < 0.01, and ***P < 0.001.
Supplier Page from Abcam for Anti-FGF2 antibody [EP1735]