Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade ab8895 from Abcam

Product Specs
ItemAnti-Histone H3 (mono methyl K4) antibody - ChIP Grade
CompanyAbcam
Price Supplier Page View Supplier Product Page
Catalog Numberab8895
Antibody TypePrimary
HostRabbit
IsotypeIgG
ClonalityPolyclonal
ApplicationsChIP, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Quantity50 UG
Conjugate/TagUnconjugated
Concentration1 mg/ml
ModificationsMono Methyl-K4
Research AreaEpigenetics
Gene NameHIST1H3A
ReactivityCow, Human
TargetHistone H3

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Website: http://www.abcam.com

(1) Sequential deregulation of histone marks, chromatin accessibility and gene expression in response to PROTAC-induced degradation of ASH2LSci RepDecember 19, 2023Mirna Barsoum, Roksaneh Sayadi-Boroujeni, Alexander T. Stenzel, Philip Bussmann, Juliane Lüscher-Firzlaff, Bernhard Lüscher#5019,AB_1950350,WB/IF γ-H2A.X,Mouse,MC,Millipore 05-636,AB_309864,WB H3,Rabbit,PC,Abcam #ab1791,AB_302613,WB/ChIP H3K4me1,Rabbit,PC,Abcam #ab8895,AB_306847,WB/ChIP H3K4me3,Rabbit,PC,Abcam #ab8580,AB_306649,WB/ChIP H3K27ac,Rabbit,PC,Abcam #ab4729,AB_2118291,WB/ChIP H3K27me3,Rabbit,PC,Antibodies

(2) Genome-wide identification of transcriptional enhancers during human placental development and association with function, differentiation, and disease†Biology of ReproductionDecember 11, 2023David M Owen, Minjung Kwon, Xuan Huang, Anusha Nagari, Tulip Nandu, W Lee KrausDynabeads (Invitrogen, 10001D) before incubation overnight with 2.5 μg of antibody: H3K4me1 (Abcam, ab8895) or H3K27ac (Abcam, ab4729). ChIP-seq libraries were constructed as described previously [51]. Next-generation sequencing

(3) Distinct Roles for COMPASS Core Subunits Set1, Trx, and Trr in the Epigenetic Regulation of Drosophila Heart DevelopmentInt J Mol SciDecember 9, 2023Jun-Yi Zhu, Hangnoh Lee, Xiaohu Huang, Joyce van de Leemput, Zhe Hanshowed clear expression in the neural nuclei [10]. Here, rabbit anti-H3K4me1 (Abcam, Cambridge, UK; ab8895), rabbit anti-H3K4me2 (Abcam, Cambridge, UK; ab7766), and rabbit anti-H3K4me3 (ActiveMotif, Carlsbad,

(4) Cotranscriptional demethylation induces global loss of H3K4me2 from active genes in ArabidopsisThe EMBO JournalDecember 1, 2023Shusei Mori, Satoyo Oya, Mayumi Takahashi, Kazuya Takashima, Soichi Inagaki, Tetsuji Kakutanichromatin solution was incubated with 1–2 μg of antibodies overnight at 4°C. Antibodies used are H3K4me1 (ab8895; Abcam), H3K4me2 (ab32356; Abcam), H3K4me3 (ab8580; Abcam), and anti‐H3 (ab1791; Abcam), H3K36me2 (MABI0332;

(5) ΔNp63α facilitates proliferation and migration, and modulates the chromatin landscape in intrahepatic cholangiocarcinoma cellsCell Death & DiseaseNovember 27, 2023Anghui Peng, Xiaowen Lin, Quanli Yang, Yihao Sun, Ruiyan Chen, Bing Liu, Xinyang YuH3K4me3 was performed using 3 μg each of H3K27ac (ab4729; Abcam), H3K27me3 (ab6002; Abcam), H3K4me1 (ab8895; Abcam), and H3K4me3 (04-745; Merck Millipore) antibody, respectively. Sequencing libraries were prepared

(6) Nuclear miR-150 enhances hepatic lipid accumulation by targeting RNA transcripts overlapping the PLIN2 promoteriScienceOctober 20, 2023Jiao Luo, Yanan Ji, Ningning Chen, Ge Song, Shuyue Zhou, Xuan Niu, Dianke YuChIP),Abcam,Cat# ab177178; RRID: AB_2828007 Anti-Histone H3 (mono methyl K4) antibody (for ChIP),Abcam,Cat# ab8895; RRID: AB_306847 Anti-Histone H3 (tri methyl K4) antibody (for ChIP),Abcam,Cat# ab8580; RRID: AB_306649 Anti-RNA polymerase II

(7) Dissecting transcription of the 8q24-MYC locus in prostate cancer recognizes the equilibration between androgen receptor direct and indirect dual-functionsJournal of Translational MedicineOctober 12, 2023Ju Guo, Zhao Wei, Tianwei Jia, Liyang Wang, Nuosu Nama, Jiaqian Liang, Xinghua Liao, Xiaming Liu, Yanfei Gao, Xiaoqiang Liu, et al.(A301-985A, Bethyl, Montgomery, TX, USA), H3K27ac (C15410196, DIAGENODE, Denville, NJ, USA), H3K4me1 (Abcam, ab8895), H3K4me2 (Abcam, ab7766), H3K9me1 (Abcam, ab9045), H3K9me2 (Abcam, ab1220), H3ac (Thermo Fisher, 39139),

(8) MUC1-C intersects chronic inflammation with epigenetic reprogramming by regulating the set1a compass complex in cancer progressionCommunications BiologyOctober 11, 2023Atrayee Bhattacharya, Atsushi Fushimi, Keyi Wang, Nami Yamashita, Yoshihiro Morimoto, Satoshi Ishikawa, Tatsuaki Daimon, Tao Liu, Song Liu, Mark D. Long, et al.Cell Signaling Technology (CST), Danvers, MA, USA), anti-MUC1-C (#16564S, 1:1000 dilution, CST), anti-H3K4me1 (#ab8895, 1:1000 dilution, Abcam), anti-H3K4me3 (#ab8580, 1:1000 dilution; Abcam), anti-H3 (#9715S, 1:1000 dilution;

(9) Epidrugs as Promising Tools to Eliminate Plasmodium falciparum Artemisinin-Resistant and Quiescent ParasitesPharmaceuticsOctober 10, 2023Thibaud Reyser, Lucie Paloque, Michel Nguyen, Jean-Michel Augereau, Matthew John Fuchter, Marie Lopez, Paola B. Arimondo, Storm Hassell-Hart, John Spencer, Luisa Di Stefano, et al.Cambridge, UK) at 1:20,000 dilution. The following primary antibodies were used: anti-H3K4me1 (Abcam, ab8895); anti-H3K4me2 (Abcam, ab35356); anti-H3K9me2 (Abcam, ab1220); anti-H3K9me3 (Millipore, 07-442, Burlington,

(10) Transcriptional network governing extraembryonic endoderm cell fate choiceDevelopmental BiologyOctober 1, 2023Paula Duyen Pham, Hanbin Lu, Han Han, Jeff Jiajing Zhou, Aarushi Madan, Wenqi Wang, Cornelis Murre, Ken W.Y. Choanti-H3K27ac (MABE647, EMD Millipore Sigma), anti-H3K4me3 (39159, Active Motif), and anti-H3K4me1 (ab8895, Abcam). Flow cytometry Cells were dissociated and resuspended in media for analyzing the expression

(11) Epigenetic dynamics during capacitation of naïve human pluripotent stem cellsScience AdvancesSeptember 29, 2023João Agostinho de Sousa, Chee-Wai Wong, Ilona Dunkel, Thomas Owens, Philipp Voigt, Adam Hodgson, Duncan Baker, Edda G. Schulz, Wolf Reik, Austin Smith, et al.antibodies were used: for Fig. 2 and fig. S4: H3 (1:1000; Abcam, #ab1791), H3K4me1 (1:1000; Abcam, #ab8895), H3K4me3 (1:1000; Abcam, #ab8580), H3K27me3 (1:1000; Cell Signaling Technology, #C36B11), H3K27ac (1:1000;

(12) Division of labor among H3K4 Methyltransferases Defines Distinct Facets of Homeostatic PlasticitybioRxivSeptember 28, 2023Takao Tsukahara, Saini Kethireddy, Katherine Bonefas, Alex Chen, Brendan LM Sutton, Yali Dou, Shigeki Iwase, Michael A. Suttonblocked with 2 % bovine albumin serum for 1 h in room temperature. Primary antibodies for H3K4me1 (Abcam ab8895, 1:300), H3K4me3 (Abcam ab8580, 1:300), MAP2 (Sigma Aldrich, 1:5000), KMT2A (Cell signaling Technology

(13) RNA polymerase II CTD is dispensable for transcription and required for termination in human cellsEMBO reportsSeptember 6, 2023Yousra Yahia, Alexia Pigeot, Amal Zine El Aabidine, Nilay Shah, Nezih Karasu, Ignasi Forné, Stefan Krebs, Helmut Blum, Cyril Esnault, Tom Sexton, et al.for western blot analysis. For ChIP‐seq experiments, anti‐(HA)‐tag antibody (ab9110, Abcam), anti‐H3K4me1 (ab8895, Abcam), anti‐H3K4me3 (ab8580, Abcam), and anti‐CTCF antibody (C15410210, Diagenode) were used. Construction

(14) Phylogenetic modeling of enhancer shifts in African mole-rats reveals regulatory changes associated with tissue-specific traitsGenome ResearchSeptember 1, 2023Elise Parey, Diego Fernandez-Aroca, Stephanie Frost, Ainhoa Uribarren, Thomas J. Park, Markus Zöttl, Ewan St. John Smith, Camille Berthelot, Diego Villarexperiment using antibodies against H3K4me3 (Millipore 05-1339), H3K27ac (Abcam ab4729), H3K4me1 (Abcam ab8895), TBX5 (Insight bio sc-515536), RXRA (sc-553), FOXA1 (ab70382), HNF4A (ARP31946), CEBPA (sc-9314), and

(15) NF-κB subunits direct kinetically distinct transcriptional cascades in antigen receptor-activated B cellsNature ImmunologySeptember 1, 2023Mingming Zhao, Prashant Chauhan, Cheryl A. Sherman, Amit Singh, Mary Kaileh, Krystyna Mazan-Mamczarz, Hongkai Ji, Jaimy Joy, Satabdi Nandi, Supriyo De, et al.anti-hnRNP A1 (Santa Cruz Biotechnology, sc-32301), anti-H3K27ac (Active Motif, 39133), anti-H3K4me1 (Abcam, ab8895) and anti-H3K4me3 (Abcam, ab8580). Horseradish peroxidase-coupled goat anti-mouse IgG (Santa Cruz Biotechnology,

(16) Low level of ARID1A contributes to adaptive immune resistance and sensitizes triple‐negative breast cancer to immune checkpoint inhibitorsCancer CommunicationsSeptember 1, 2023Xin‐Yu Chen, Bin Li, Ye Wang, Juan Jin, Yu Yang, Lei‐Huan Huang, Meng‐Di Yang, Jian Zhang, Bi‐Yun Wang, Zhi‐Ming Shao, et al.immunoprecipitated by either control IgG, H3K4me3 (1:50, 9751, Cell Signaling Technology), H3K4me1 (2 μg, ab8895, Abcam), H3K27ac (1 μg, C15210016, Diagenode, New Jersey, USA), ARID1A (1:100), SWI/SNF‐related, matrix‐associated,

(17) IRF8 configures enhancer landscape in postnatal microglia and directs microglia specific transcriptional programsbioRxivAugust 22, 2023Keita Saeki, Richard Pan, Eunju Lee, Daisuke Kurotaki, Keiko Ozatofollowing antibodies in the presence of 2mM EDTA: rabbit anti-H3K27ac (ab4729, Abcam), rabbit anti-H3K4me1 (ab8895, Abcam). After antibody binding, beads were washed twice with Digitonin buffer and bound pA-MNase. After

(18) Differentiation block in acute myeloid leukemia regulated by intronic sequences of FTOiScienceAugust 18, 2023Francesco Camera, Isabel Romero-Camarero, Bradley H. Revell, Fabio M.R. Amaral, Oliver J. Sinclair, Fabrizio Simeoni, Daniel H. Wiseman, Lovorka Stojic, Tim C.P. SomervailleRRID:AB_2815140 anti-acetyl H3K27,Abcam,Cat#ab4729; RRID:AB_2118291 anti-monomethyl-H3K4,Abcam,Cat#ab8895; RRID:AB_306847 anti-MED1,Bethyl,Cat#A300-793ARRID: AB_577241 5-methylcytosine monoclonal antibody

(19) Inter and transgenerational impact of H3K4 methylation in neuronal homeostasisLife Science AllianceAugust 1, 2023Steffen Abay-Nørgaard, Marta Cecylia Tapia, Mandoh Zeijdner, Jeonghwan Henry Kim, Kyoung Jae Won, Bo Porse, Anna Elisabetta Salcinifollowing antibodies were used: anti-H3K4me3 (C42D8; 1:750; Cell Signaling Technology); anti-H3K4me1 (ab8895; 1:1,000; Abcam); anti-H3K4me2 (07-030; 1:1,000; Millipore) anti-H3 (ab1791; 1:10,000; Abcam); anti-H3K9me3

(20) Mixed-Lineage Leukemia 1 Inhibition Enhances the Differentiation Potential of Bovine Embryonic Stem Cells by Increasing H3K4 Mono-Methylation at Active PromotersInternational journal of molecular sciencesJuly 25, 2023Li C, Han X, Wang J, Liu F, Zhang Y, Li Z, Lu Z, Yue Y, Xiang J, Li X.this experiment included anti-H3 (4620S, Cell Signaling Technology, Boston, MA, USA), anti-H3K4me1 (ab8895, Abcam, Cambridge, MU, USA), anti-H3K4me2 (ab32356, Abcam), anti-H3K27me3 (ab6002, Abcam), anti-GAPDH

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1/15/2015Western blot review for anti-histone H3 (mono methyl K4) antibody Laura LecceThis product was used to determine if changes in H3H4me1 are associated with endothelial-mesenchymal-transition. This antibody was recommended to me by a co-worker.

Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade from Abcam

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Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade from Abcam

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