Fig 1: (A) show the expression and distribution of representative multicolored immunofluorescence of IFNα, IFNRA, IP10, IRF3, IRF5, and IRF7 in liver tissue of the deceased twin and control groups. IFNα, IFNAR, and IP10 as Panel 1(green represents IP10, yellow represents IFNα, orange represents IFNRA, pink represents CD68, and blue represents the nucleus), and IRF3, IRF5, and IRF7 as Panel 2(green represents IRF7, yellow represents IRF5, orange represents IRF3, pink represents CD68 and blue represents the nucleus). (B) displayed statistical analysis based on (A). (C) displayed IP10 and IFNα correlation with IRF7, IRF5, IRF3, and IFNAR, respectively. N.S, no significace, * P value <0.05, ** P value <0.01, *** P value <0.001 and **** P value <0.001.
Fig 2: 3-MA inhibits cytokines-induced IP-10 expression. (A) HUVECs were pretreated with or without 5 mmol/L 3-MA for 30 min followed by 10 ng/mL IL-1β or 10 ng/mL TNFα for 24 h. Conditioned medium was collected and cytokines in the medium were analyzed by an antibody array. Upper panel shows representative dot blots of 80 cytokines/chemokines. Arrows indicate dots of IP-10. The lower panel shows the place of each cytokine within the array. (B) Densitometric analysis of IP-10 in the dot blots (n = 2). (C) IP-10 in the medium was analyzed by ELISA. Each bar denotes mean ± SD of three independent experiments. *Indicates P < 0.05.
Fig 3: IFNy induces IP-10 expression in pericytes and is not affected by TGFβ1 or M-CSF.Following IFNy treatment, IP-10 expression is significantly increased in pericytes from basal levels. Simultaneous treatment with either TGFβ1 or M-CSF does not affect the levels of IP-10 expression induced by IFNy (N = 12).
Fig 4: (A-C) show the expression and distribution of representative immunofluorescence of E11 in the intestine, lungs, and liver of the deceased twins and control group. Green represents E11, and blue represents the nucleus. White arrows indicate E11. (D) displayed statistical analysis based on immunofluorescence in (A-C, E) show the expression and distribution of IP10 in the different tissues. Red represents IP10, and blue represents the nucleus. White arrows indicate IP10. (F) displayed statistical analysis based on the observed immunofluorescence in (E). N.S, no significace, * P value <0.05, ** P value <0.01, *** P value <0.001 and **** P value <0.001.
Fig 5: IP-10 is expressed by microglia and astrocytes in primary adult human mixed glial cultures.A) Following IFNy treatment (1 ng/ml, 96 h) IP-10 expression (green) is co-localised with CD45-immunopositive microglia (red). All nuclei are labelled with Hoechst (blue). Hoechst, IP-10 and CD45 and overlaid in the far right image. B) GFAP-immunopositive astrocytes (red) express IP-10 following IFNy treatment. Hoechst, IP-10 and GFAP are overlaid in the far right image. Scale bar = 100 µm. Arrows indicate high levels of IP-10 expression and insets show close-up examples of cells expressing IP-10.
Supplier Page from Abcam for Anti-IP10 antibody