Collagen I Antibody

Product Specs
ItemCollagen I Antibody - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberNB600-408-0.1mg
Antibody TypePrimary Antibodies
ReactivityHu, Ms, Rb, Rt, Ba, Bv, Ck, Pg, Zf
NCBI Gene AliasesCAFYD, EDSC, EDSARTH1, OI4, OI3, OI2, OI1
NCBI Full Gene Namecollagen type I alpha 1 chain
ApplicationsWB, ELISA, ICC, IF, IHC, IHC-fr, IHC-p, IP, Array, FLISA
PurityImmunogen affinity purified
ClonalityPolyclonal
ImmunogenCollagen I from human and bovine placenta (Uniprot: P02452)
HostRabbit
IsotypeIgG
TargetCollagen I
Format0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Conjugate/TagUnconjugated
Quantity0.1 mg (also 0.025 mg)
Research AreaCell Biology, Cellular Markers, Extracellular Matrix, Signal Transduction, Stem Cells
StorageStore at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has expected cross-reactivity with Type III and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.

Sizes Available: 0.1 mg (also 0.025 mg)

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Questions and Answers

What applications has the Collagen I alpha 1 Antibody been used for in research?

The Collagen I alpha 1 Antibody has been shown to work in various applications, including immunohistochemistry and Western blotting, as evidenced by its citation in 116 scientific articles.

Can this antibody be used to study collagen-related diseases?

Yes, the Collagen I alpha 1 Antibody can be utilized to investigate collagen-related pathologies, such as fibrosis, as it plays a crucial role in the structure and function of the extracellular matrix, which is often implicated in fibrotic diseases.

Is there any evidence of this antibody being used in studies related to liver conditions?

The antibody has been referenced in studies examining liver conditions, including its role in non-alcoholic fatty liver disease (NAFLD), where collagen deposition is a key feature of the disease progression, as highlighted in research articles.

How does the Collagen I alpha 1 Antibody contribute to understanding cellular mechanisms in fibrosis?

The antibody aids in elucidating the cellular mechanisms of fibrosis by allowing researchers to visualize and quantify collagen deposition in tissues, thereby providing insights into the fibrotic response and the role of collagen in tissue remodeling during disease states.

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Effects of Compression on Extracellular Matrix Synthesis by Chondrocytes and Chondrosarcoma CellsCartilageJuly 17, 2026Carlo Alberto Paggi, Isa Porsul, Séverine Le Gac, Marcel Karperienantibodies were ACAN (Anti-ACAN antibody [6-B-4] (ab3778), Abcam, Cambridge, UK), COL1 (Collagen I Antibody (NB600-408), Novus biological Abingdon, UK), COL2 (COL2A1 Antibody (ab34712), Abcam) and COL6 (Anti-Collagen VI

(2) Atg5 deficiency alters myofibroblast accumulation and alveolar regeneration in lung fibrosisStem Cell ReportsJuly 14, 2026Xue Li, Guiying Xu, Qi Wang, Qingwen Ma, Xue Liu, Hongwei Li, Sisi Wang, Yimeng Liu, Yueyue Jin, Xuan Miao, et al.14-5381-85; RRID: AB_1210505 Anti-α-SMA,Santa Cruz,Cat#: SC32251; RRID: AB_262054 Anti-Collagen,Novus,Cat#: NB600-408; RRID: AB_10000511 Anti-Ki67,Invitrogen,Cat#: 14-5698-82; RRID: AB_10854564 Anti-GAPDH,CST,Cat#: 2118;

(3) Maternal Exposure to Wood-Smoke-Derived PM2.5 Is Associated with Delayed Fetal Neurocranial Intramembranous Ossification in a Rat ModelInternational Journal of Molecular SciencesJune 24, 2026Paulo Salinas, Francisca Villarroel, Luis Astorga, Paula Cerda, Eva Rojas, Aliro MaulénNBP2-67415, Novus Biologicals; validated for rat), and type I collagen (COL-1, 1:100, rabbit polyclonal, cat# NB600-408, Novus Biologicals; validated for rat). Negative controls (primary antibody omitted) and positive controls

(4) Targeting fibroblast TXNDC5 resolves tumor desmoplasia and PD-1 resistance in colorectal cancer with mesenchymal traitsNature CommunicationsJune 5, 2026Kai-Lin Cheng, Chih-I Chen, Shu-Han Yu, Huai-Wen Liang, Yi-Wei Tsai, Chen-Ting Hung, Yu-Shan Lin, Gang-Hui Lee, Po-Yu Chen, Ming-Jer Tang, et al.following cryopreservation and immunofluorescence staining with anti-Collagen I (Novus Biologicals, # NB600-408, polyclonal, 1:200) to identify regions of interest (ROIs). Tumor stiffness was measured immediately

(5) Cell-dependent contributions of thrombospondin-1 to the rupture of abdominal aortic aneurysm in miceCardiovascular ResearchFebruary 14, 2026Ting Zhou, Huan Yang, Jooyong Kim, Carmel Assa, Elise DeRoo, Jack Bontekoe, Brian Burkel, Suzanne Ponik, Hong S. Lu, Alan Daugherty, et al.and incubated at 4°C overnight with primary antibodies anti-CD68 (MCA1957GA, Bio-Rad), anti-Col1a1 (NB600–408, Novus Biologicals), or anti-MYH11 (ab53219, abcam). Normal goat or rabbit IgG was used as negative

(6) A Rat Model Investigation of Enhanced Facial Rejuvenation via PCL Microsphere-Induced Superior Collagen Neogenesis in the Supraperiosteal PlaneAesthetic Plastic SurgeryFebruary 9, 2026Pai-Nien Chu, Shengjun Zhu, Xia Dai, Shihui Zhuab8448) were purchased from Abcam (Cambridge, UK). Rabbit anti-rat type I collagen primary antibody (NB600-408) was obtained from Novus Biologicals (Littleton, CO, USA). Mouse Leptin Receptor (LepR) biotinylated

(7) A preliminary preclinical assessment of macromolecular crowding in tissue engineeringScience ProgressJanuary 21, 2026Kyriakos Spanoudes, Laura Trujillo Cubillo, Stefanie H. Korntner, Diana Gaspar, Dimitrios I. Zeugolisprotocols.47,48 In brief, cell layers were washed, fixed, blocked, incubated with mouse anti-collagen type I (NB600-408, Novus Biologicals, USA), rabbit anti-collagen type III (Ab7778, Abcam, Ireland) or rabbit anti-collagen

(8) Hypoglycemia induces brain metabolic reprogramming and neurodegeneration via serum response factor and myocardin-related transcription factor-ASignal Transduct Target TherDecember 17, 2025Minjeong Jang, Hyung Jin Choi, Hae-June Lee, Hong Nam KimSanta Cruz), anti-ß-tubulin (sc-5274), and anti-Lamin A/C (sc-7292, all from Santa Cruz) Biotechnology Inc.), (NB600-408, Novus), horseradish peroxide (HRP)-linked anti-rabbit IgG (7074, Cell Signaling), and HRP-linked anti-mouse

(9) Relaxin-2 drives regenerative healing and suppresses scar formationbioRxivDecember 12, 2025Amanda K. Williamson, Katherine M. Hohl, Jack R. Kirsch, Rosalynn M. Nazarian, Thomas P. Schaer, Daniel S. Roh, Mark W. GrinstaffSensitivity substrate on a ChemiDoc XRS+ (Bio-Rad). Primary antibodies: Collagen-I (Novus Biologicals, NB600-408), pro-collagen1α1 (R&D Systems, AF6220), collagen-III (Novus Biologicals, NB600-594), αSMA (Abcam, ab7817),

(10) Mitochondria Derived From iPSCs Control by Reciprocal Regulation of Th17/Treg and Anti-FibrosisImmune NetworkDecember 8, 2025Jin Seok Woo, Hanbi Lee, JooYeon Jhun, SeungCheon Yang, Yeon Su Lee, Yoo-Jin Shin, Sun Woo Lim, Bo-In Lee, Chul Woo Yang, Byung Ha Chung, et al.NB600-633:diluted 1:400), anti- α-SMA (Abcam, ab7817:diluted 1:1,000), anti-Collagen I (Novus Biologicals, NB600-408:diluted 1:200), anti-Fibronectin (Abcam, ab2413:diluted 1:50) and anti-connective tissue growth factor

(11) Endothelial adenosine receptor 2A loss alleviates diabetic vascular calcification by blocking CREB1-SNAI1-driven EndMTPharmacological ResearchNovember 1, 2025Yaqi Zhou, Dingwei Zhao, Qian Ma, Jiean Xu, Yongfeng Cai, Qiuhua Yang, Qingen Da, Kian Sheridan, Chunxiang Zhang, Clint L. Miller, et al.ab209484, Abcam, Cambridge, UK), BGLAP (1 μg/mL; Cat# ab93876, Abcam, Cambridge, UK), COL1A1 (1 μg/mL; Cat# NB600408, Novus Biologicals, CO, USA), and GAPDH (0.5 μg/mL; Cat# 5174S, Cell Signaling Technology, MA, USA).

(12) Imeglimin attenuates liver fibrosis by inhibiting vesicular ATP release from hepatic stellate cellsFEBS LettersSeptember 15, 2025Seiji Nomura, Lixiang Wang, Nao Hasuzawa, Ayako Nagayama, Sawako Moriyama, Kenji Ashida, Yoshinori Moriyama, Masatoshi Nomura, Ken Yamamotowith the following primary antibodies: anti‐α‐SMA (A5228; Sigma‐Aldrich), anti‐Collagen I (COL1A1, NB600‐408; Novus Biologicals, Centennial, CO, USA), anti‐Transforming growth factor‐beta 1 (TGF‐β1, ab215715;

(13) Harnessing Lactiplantibacillus plantarum EP21 and its membrane vesicles to inhibit myopia developmentGut MicrobesAugust 1, 2025Chi-Fong Lin, Yu-An Hsu, Yung-Lan Chou, Ying-Chi Chen, En-Shyh Lin, Peng-Tai Tien, Jamie Jiin-Yi Chen, Ming-Yen Wu, Chia-Hung Lin, Hui-Ju Lin, et al.# ab66043; RRID:AB_1143428), MMP2 (Abcam; Cat. # ab97779; RRID:AB_10696122), COL1A1 (Novus; Cat. # NB600–408; RRID:AB_10000511), ChromPure Rat IgG (Jackson ImmunoResearch Labs; Cat. # 012–000–003; RRID:AB_2337136)

(14) Primary Mouse Choroidal Endothelial Cell CultureBIO-PROTOCOLJune 20, 2025Qiuhua Yang, Yongfeng Cai, Qian Ma, Yuqing HuoSM22α antibody (Abcam, catalog number: ab14106) 8. COL1 antibody (Novus Biologicals, catalog number: NB600-408) 9. CD31 antibody (Santa Cruz Biotechnology, catalog number: 376764) 10. β-actin antibody (Santa Cruz Biotechnology

(15) Primary Mouse Choroidal Endothelial Cell CultureBio ProtocJune 20, 2025Qiuhua Yang, Yongfeng Cai, Qian Ma, Yuqing HuoSM22α antibody (Abcam, catalog number: ab14106) 8. COL1 antibody (Novus Biologicals, catalog number: NB600-408) 9. CD31 antibody (Santa Cruz Biotechnology, catalog number: 376764) 10. β-actin antibody (Santa Cruz Biotechnology

(16) The Characterization of Serum-Free Media on Human Mesenchymal Stem Cell FibrochondrogenesisBioengineering (Basel)May 19, 2025Ka Yu Carissa Kwan, Ke Li, Yu Yang Wang, Wai Yi Tse, Chung Yan Tong, Xu Zhang, Dan Michelle Wang, Dai Fei Elmer KerCat: Ab15580) or 2 μg/mL rabbit anti-collagen type I (Novus Biologicals, Centennial, CO, USA, Cat: NB600-408), 5 μg/mL mouse anti-collagen type II (Invitrogen, Carlsbad, CA, USA, Cat: MA5-12789) and 5 μg/mL mouse

(17) Enhancing Cartilage Repair in Osteoarthritis Using Platelet Lysates and Arthroscopic MicrofractureDrug Des Devel TherMay 13, 2025Haiyan Zhang, Dipeng Li, Wei Zheng, Jiaqing Hua, Zuxiang Chen, Wenting Xu, Jianing Zhu, Yue Wang, Xiaotian Chen, Huixin Chen, et al.Mix (low ROX) kit was purchased from Bimake (TX, USA). The primary antibodies collagen type I (Col1, NB600-408), collagen type III (Col3, NB600-594), Aggrecan (NB100-74350), Fibronectin (NBP1-91258) and vascular

(18) Enhancing Cartilage Repair in Osteoarthritis Using Platelet Lysates and Arthroscopic MicrofractureDrug Des Devel TherMay 13, 2025Haiyan Zhang, Dipeng Li, Wei Zheng, Jiaqing Hua, Zuxiang Chen, Wenting Xu, Jianing Zhu, Yue Wang, Xiaotian Chen, Huixin Chen, et al.Mix (low ROX) kit was purchased from Bimake (TX, USA). The primary antibodies collagen type I (Col1, NB600-408), collagen type III (Col3, NB600-594), Aggrecan (NB100-74350), Fibronectin (NBP1-91258) and vascular

(19) Desmoplastic tumor priming using clinical-stage corticosteroid liposomesCell BiomaterApril 22, 2025Tarun Ojha, Gideon J.L. Schaefer, Rahaf Mihyar, Vertika Pathak, Josef Ehling, Elena Rama, Federica De Lorenzi, Asmaa Said Elshafei, Diana Moeckel, Sara Elsafy, et al.(Cell Signaling #3169), hyaluronan antibody (Abcam #53842), collagen I antibody (Novus Biologicals #NB600-408), caspase-3 antibody (abcam #ab49822), MRC-1 (Acris #SM1857P), and phosphorylated histone H2A.X (Cell

(20) Hydrogels with programmed spatiotemporal mechanical cues for stem cell-assisted bone regenerationNat CommunApril 16, 2025Bin Xue, Zhengyu Xu, Lan Li, Kaiqiang Guo, Jing Mi, Haipeng Wu, Yiran Li, Chunmei Xie, Jing Jin, Juan Xu, et al.phosphatase antibody (ALP, cat: NB600-540, Novus, USA), rabbit polyclonal collagen I antibody (col I, cat: NB600-408, Novus, USA), 4’,6-diamidino-2-phenylindole (DAPI, cat: MBD0015, Sigma‒Aldrich, China), Alexa fluor

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