Collagen I Antibody

Product Specs
ItemCollagen I Antibody - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberNB600-408-0.1mg
Quantity0.1 mg (also 0.025 mg)
ApplicationsIF/IHC,ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,SDS-Page,Fluorophore-linked immunosorbent assay,Microarray,Immunohistochemistry Whole-Mount,Immunofluorescence,Immunocytochemistry,Knockdown Validated,Immunocytochemistry/ Immunofluorescence
Conjugate/TagUnconjugated
Format0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
ReactivityAvian - Chicken,Bacteria,Bovine,Human,Mouse,Porcine,Rabbit,Rat,Fish - Danio rerio (Zebrafish)
TargetCollagen I
IsotypeIgG
HostRabbit
ImmunogenCollagen I from human and bovine placenta (Uniprot: P02452)
ClonalityPolyclonal
PurityImmunogen affinity purified
NCBI Full Gene Namecollagen type I alpha 1 chain
NCBI Gene AliasesCAFYD, EDSC, EDSARTH1, OI4, OI3, OI2, OI1
Research AreaCell Biology, Cellular Markers, Extracellular Matrix, Signal Transduction, Stem Cells
Antibody TypePrimary Antibodies
StorageStore at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

What applications has the Collagen I alpha 1 Antibody been used for in research?

The Collagen I alpha 1 Antibody has been shown to work in various applications, including immunohistochemistry and Western blotting, as evidenced by its citation in 116 scientific articles.

Can this antibody be used to study collagen-related diseases?

Yes, the Collagen I alpha 1 Antibody can be utilized to investigate collagen-related pathologies, such as fibrosis, as it plays a crucial role in the structure and function of the extracellular matrix, which is often implicated in fibrotic diseases.

Is there any evidence of this antibody being used in studies related to liver conditions?

The antibody has been referenced in studies examining liver conditions, including its role in non-alcoholic fatty liver disease (NAFLD), where collagen deposition is a key feature of the disease progression, as highlighted in research articles.

How does the Collagen I alpha 1 Antibody contribute to understanding cellular mechanisms in fibrosis?

The antibody aids in elucidating the cellular mechanisms of fibrosis by allowing researchers to visualize and quantify collagen deposition in tissues, thereby providing insights into the fibrotic response and the role of collagen in tissue remodeling during disease states.

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) A Rat Model Investigation of Enhanced Facial Rejuvenation via PCL Microsphere-Induced Superior Collagen Neogenesis in the Supraperiosteal PlaneAesthetic Plastic SurgeryFebruary 9, 2026Pai-Nien Chu, Shengjun Zhu, Xia Dai, Shihui Zhuab8448) were purchased from Abcam (Cambridge, UK). Rabbit anti-rat type I collagen primary antibody (NB600-408) was obtained from Novus Biologicals (Littleton, CO, USA). Mouse Leptin Receptor (LepR) biotinylated

(2) A preliminary preclinical assessment of macromolecular crowding in tissue engineeringScience ProgressJanuary 21, 2026Kyriakos Spanoudes, Laura Trujillo Cubillo, Stefanie H. Korntner, Diana Gaspar, Dimitrios I. Zeugolisprotocols.47,48 In brief, cell layers were washed, fixed, blocked, incubated with mouse anti-collagen type I (NB600-408, Novus Biologicals, USA), rabbit anti-collagen type III (Ab7778, Abcam, Ireland) or rabbit anti-collagen

(3) Hypoglycemia induces brain metabolic reprogramming and neurodegeneration via serum response factor and myocardin-related transcription factor-ASignal Transduct Target TherDecember 17, 2025Minjeong Jang, Hyung Jin Choi, Hae-June Lee, Hong Nam KimSanta Cruz), anti-ß-tubulin (sc-5274), and anti-Lamin A/C (sc-7292, all from Santa Cruz) Biotechnology Inc.), (NB600-408, Novus), horseradish peroxide (HRP)-linked anti-rabbit IgG (7074, Cell Signaling), and HRP-linked anti-mouse

(4) Mitochondria Derived From iPSCs Control by Reciprocal Regulation of Th17/Treg and Anti-FibrosisImmune NetworkDecember 8, 2025Jin Seok Woo, Hanbi Lee, JooYeon Jhun, SeungCheon Yang, Yeon Su Lee, Yoo-Jin Shin, Sun Woo Lim, Bo-In Lee, Chul Woo Yang, Byung Ha Chung, et al.NB600-633:diluted 1:400), anti- α-SMA (Abcam, ab7817:diluted 1:1,000), anti-Collagen I (Novus Biologicals, NB600-408:diluted 1:200), anti-Fibronectin (Abcam, ab2413:diluted 1:50) and anti-connective tissue growth factor

(5) Imeglimin attenuates liver fibrosis by inhibiting vesicular ATP release from hepatic stellate cellsFEBS LettersSeptember 15, 2025Seiji Nomura, Lixiang Wang, Nao Hasuzawa, Ayako Nagayama, Sawako Moriyama, Kenji Ashida, Yoshinori Moriyama, Masatoshi Nomura, Ken Yamamotowith the following primary antibodies: anti‐α‐SMA (A5228; Sigma‐Aldrich), anti‐Collagen I (COL1A1, NB600‐408; Novus Biologicals, Centennial, CO, USA), anti‐Transforming growth factor‐beta 1 (TGF‐β1, ab215715;

(6) Interferon-driven CAF reprogramming augments immunogenic response to neoadjuvant radiotherapy in colorectal cancerCell reports. MedicineAugust 19, 2025Huang L, Lu W, Wu R, Li Y, Ou Z, Chen J, Liu Y, Yang W, Xue W, Mu P, et al.AB_2895112 Collagen III alpha 1/COL3A1 Antibody,NOVUS,Cat#NB600-594; RRID: AB_10001330 Collagen I Antibody,NOVUS,Cat#NB600-408; RRID: AB_10000511 Collagen I alpha 1 Antibody (COL-1),NOVUS,Cat#NB600-450; RRID: AB_10124390 Podoplanin

(7) Harnessing Lactiplantibacillus plantarum EP21 and its membrane vesicles to inhibit myopia developmentGut MicrobesAugust 1, 2025Chi-Fong Lin, Yu-An Hsu, Yung-Lan Chou, Ying-Chi Chen, En-Shyh Lin, Peng-Tai Tien, Jamie Jiin-Yi Chen, Ming-Yen Wu, Chia-Hung Lin, Hui-Ju Lin, et al.# ab66043; RRID:AB_1143428), MMP2 (Abcam; Cat. # ab97779; RRID:AB_10696122), COL1A1 (Novus; Cat. # NB600–408; RRID:AB_10000511), ChromPure Rat IgG (Jackson ImmunoResearch Labs; Cat. # 012–000–003; RRID:AB_2337136)

(8) Multispectral Pulsed Photobiomodulation Enhances Diabetic Wound Healing via Focal Adhesion-Mediated Cell Migration and Extracellular Matrix RemodelingInternational journal of molecular sciencesJune 27, 2025Choi J, Ban MJ, Gil CH, Hur SS, Anggradita LD, Kim MK, Son JW, Kim JE, Hwang Y.Irvine, CA, USA), alpha-smooth muscle actin (α-SMA; ab5694; Abcam, Cambridge, UK), and collagen I (#NB600-408; Novus Biologicals, Centennial, CO, USA). Next, sections were incubated with HRP-conjugated secondary

(9) Primary Mouse Choroidal Endothelial Cell CultureBIO-PROTOCOLJune 20, 2025Qiuhua Yang, Yongfeng Cai, Qian Ma, Yuqing HuoSM22α antibody (Abcam, catalog number: ab14106) 8. COL1 antibody (Novus Biologicals, catalog number: NB600-408) 9. CD31 antibody (Santa Cruz Biotechnology, catalog number: 376764) 10. β-actin antibody (Santa Cruz Biotechnology

(10) The Characterization of Serum-Free Media on Human Mesenchymal Stem Cell FibrochondrogenesisBioengineering (Basel)May 19, 2025Ka Yu Carissa Kwan, Ke Li, Yu Yang Wang, Wai Yi Tse, Chung Yan Tong, Xu Zhang, Dan Michelle Wang, Dai Fei Elmer KerCat: Ab15580) or 2 μg/mL rabbit anti-collagen type I (Novus Biologicals, Centennial, CO, USA, Cat: NB600-408), 5 μg/mL mouse anti-collagen type II (Invitrogen, Carlsbad, CA, USA, Cat: MA5-12789) and 5 μg/mL mouse

(11) Enhancing Cartilage Repair in Osteoarthritis Using Platelet Lysates and Arthroscopic MicrofractureDrug Des Devel TherMay 13, 2025Haiyan Zhang, Dipeng Li, Wei Zheng, Jiaqing Hua, Zuxiang Chen, Wenting Xu, Jianing Zhu, Yue Wang, Xiaotian Chen, Huixin Chen, et al.Mix (low ROX) kit was purchased from Bimake (TX, USA). The primary antibodies collagen type I (Col1, NB600-408), collagen type III (Col3, NB600-594), Aggrecan (NB100-74350), Fibronectin (NBP1-91258) and vascular

(12) Desmoplastic tumor priming using clinical-stage corticosteroid liposomesCell BiomaterApril 22, 2025Tarun Ojha, Gideon J.L. Schaefer, Rahaf Mihyar, Vertika Pathak, Josef Ehling, Elena Rama, Federica De Lorenzi, Asmaa Said Elshafei, Diana Moeckel, Sara Elsafy, et al.(Cell Signaling #3169), hyaluronan antibody (Abcam #53842), collagen I antibody (Novus Biologicals #NB600-408), caspase-3 antibody (abcam #ab49822), MRC-1 (Acris #SM1857P), and phosphorylated histone H2A.X (Cell

(13) Hydrogels with programmed spatiotemporal mechanical cues for stem cell-assisted bone regenerationNat CommunApril 16, 2025Bin Xue, Zhengyu Xu, Lan Li, Kaiqiang Guo, Jing Mi, Haipeng Wu, Yiran Li, Chunmei Xie, Jing Jin, Juan Xu, et al.phosphatase antibody (ALP, cat: NB600-540, Novus, USA), rabbit polyclonal collagen I antibody (col I, cat: NB600-408, Novus, USA), 4’,6-diamidino-2-phenylindole (DAPI, cat: MBD0015, Sigma‒Aldrich, China), Alexa fluor

(14) Mimicking the Dystrophic Cardiac Extracellular Environment through DystroGelAdvanced Healthcare MaterialsFebruary 17, 2025Maila Chirivì, Fabio Maiullari, Marika Milan, Maria Grazia Ceraolo, Nicole Fratini, Alessandra Fasciani, Salma Bousselmi, Michael Stirm, Francesca Scalera, Francesca Gervaso, et al.bs‐10367R), cardiac Troponin T (cTnT; 1:100, Abcam, ab8295), Collagen I (Col1, 1:100, Novus Biologicals, NB600‐408) and Collagen IV (Col4, 1:100, Abcam, ab6586) antibodies diluted in 0.5% BSA solution. Finally, the

(15) Live imaging of the extracellular matrix with a glycan-binding fluorophoreNature MethodsFebruary 6, 2025Antonio Fiore, Guoqiang Yu, Jason J. Northey, Ronak Patel, Thomas A. Ravenscroft, Richard Ikegami, Wiert Kolkman, Pratik Kumar, Tanya L. Dilan, Virginia M. S. Ruetten, et al.33342 (Thermo Fisher Scientific, 62249), and/or 10 µg ml–1 Atto647N-anti-collagen-I (Novus Biologicals, NB600-408, fluorescently labeled as above). After 1 h of incubation at 37 °C, the tissue was mounted for imaging

(16) Osteogenic potential of silver nanoparticles in critical sized mandibular bone defects: an experimental study in white albino ratsOdontologyJanuary 10, 2025Gehad Mohamed Sabry, Nessma Sultan, Mazen Tharwat Abouelkhier, Essam Farouk SoussaImmunohistochemistry (IHC) Using a conventional streptavidin–biotin immunoperoxidase approach, collagen-I antibody (COL-I; Cat# NB600-408, Novus Biologicals) and tumor necrotic factor-alpha (TNF-alpha; Cat# Ab307164, Abcam) were used for

(17) BMP-9 mediates fibroproliferation in fibrodysplasia ossificans progressiva through TGF-β signalingEMBO molecular medicineJanuary 1, 2025Zhao C, Inada Y, Motoike S, Kamiya D, Hino K, Ikeya M.2B1.5) Mouse mAb,Thermo Fisher Scientific,#MS-235-B0 Collagen I alpha 1 Antibody,Novus Biologicals,NB600-408 Anti-F4/80 antibody [CI: A3-1],Abcam,ab6640 Anti-PDGFR alpha antibody [RM0004-3G28],Abcam,ab51875 Anti-SP7/Osterix

(18) N6-methyladenosine regulates metabolic remodeling in kidney aging through transcriptional regulator GLIS1BMC biologyDecember 31, 2024Xu L, Chen S, Fan Q, Zhu Y, Mei H, Wang J, Yu H, Chen Y, Liu F.with primary antibodies: GLIS1 (Active Motif, 61,801), α-SMA (Proteintech, 67,735–1-Ig), COL I (Novus, NB600-408), FN (Abcam, ab2413), PPARα (Novus, NB300-537), ACOX1 (Abcam, ab184032), CPT1A (Proteintech, 15,184–1-AP),

(19) Pathological characterization of lung fibrosis in Sprague–Dawley rats treated with fluoro-edenite fibres by intrapleural injectionJournal of Occupational Medicine and ToxicologyDecember 13, 2024Eva Tibaldi, Federica Gnudi, Daniele Mandrioli, Caterina Bruno, Amerigo Zona, Lucia Fazzo, Pietro Comba(alpha-SMA, #MA1-06110, Thermo Fisher Scientific, Waltham, Massachusetts, USA), collagen type I (COL1, #NB600408, Novus Biological, Centennial, Colorado, USA) and vimentin (#sc-371717, Santa Cruz Biotechnologies,

(20) Novel kinase regulators of extracellular matrix internalisation identified by high-content screening modulate invasive carcinoma cell migrationPLOS BiologyDecember 12, 2024Montserrat Llanses Martinez, Keqian Nan, Zhe Bao, Rachele Bacchetti, Shengnan Yuan, Joe Tyler, Xavier Le Guezennec, Frederic A. Bard, Elena RaineroMAPK (Rabbit PolyAb Thr180/Tyr182) from Proteintech (28796-1-AP). Primary antibody against collagen I (NB600-408) from Novus. Secondary antibodies Alexa-Fluor 488 Donkey Anti-mouse were from Fisher Scientific (A-21202),

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