Anti-FKBP12 antibody ab2918 from Abcam

Product Specs
ItemAnti-FKBP12 antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab2918
Antibody TypePrimary
IsotypeIgG
ClonalityPolyclonal
Quantity100 UL
HostRabbit
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameFKBP1A
ReactivityHuman
TargetFKBP12

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Temporal control of contact site formation reveals a relationship between mitochondrial division and Num1-mediated mitochondrial tetheringMolecular Biology of the CellOctober 1, 2023Clare S. Harper, Jason C. Casler, Laura L. Lacknerused were anti-GFP (Abclonal Rabbit anti-GFP-Tag pAb 1:2000) and anti-FKBP12 (Abcam Rabbit anti-FKBP12 ab2918 1:1000) as primaries and goat antirabbit IgG DyLight 800 (Thermo Fisher Scientific 1:15,000) as a secondary

(2) A kinesin‐based approach for inducing chromosome‐specific mis‐segregation in human cellsThe EMBO JournalMay 15, 2023My Anh Truong, Paula Cané‐Gasull, Sippe G de Vries, Wilco Nijenhuis, René Wardenaar, Lukas C Kapitein, Floris Foijer, Susanne MA Lensmilk in TBS‐0.01% Tween‐20 for 1 h, before being incubated with rabbit anti‐FKBP12 antibodies (Abcam ab2918) at 4°C overnight. Horseradish peroxidase (HRP)‐conjugated goat anti‐rabbit secondary antibodies (Bioke,

(3) Assessment of Covalently Binding Warhead Compounds in the Validation of the Cytomegalovirus Nuclear Egress Complex as an Antiviral TargetCellsApril 14, 2023Julia Tillmanns, Sigrun Häge, Eva Maria Borst, Julia Wardin, Jan Eickhoff, Bert Klebl, Sabrina Wagner, Christina Wangen, Friedrich Hahn, Eileen Socher, et al.Sigma-Aldrich, St. Louis, MO, USA), pAb-Flag (F7425, Sigma-Aldrich, St. Louis, MO, USA), pAb-FKBP12 (ab2918, Abcam, Cambridge, UK) and mAb-β-actin (A5441, Sigma-Aldrich, St. Louis, MO, USA). 2.9. Indirect Immunofluorescence

(4) Impact of aging and oxidative stress on specific components of excitation contraction coupling in regulating force generationScience AdvancesOctober 28, 2022Hongyang Xu, Bumsoo Ahn, Holly Van RemmenNKAα1 (Cell Signaling Technology, #3010), NKAα2 (Merck Millipore, #07-674), calstabin (FKBP12) (Abcam, #ab2918), GSH (ViroGen, #101-A), and TnI (Cell Signaling Technology, #4002). Statistical analysis All results

(5) BubR1 recruitment to the kinetochore via Bub1 enhances spindle assembly checkpoint signalingMolecular Biology of the CellSeptember 1, 2022Anand Banerjee, Chu Chen, Lauren Humphrey, John J. Tyson, Ajit P. Joglekar2001), 1:500 or 1:1000, sheep polyclonal; BUB3 (Sigma B7811), 1:500, rabbit polyclonal; FKBP12 (Abcam ab2918), 1:2000, rabbit polyclonal; BUBR1 (Bethyl A300-995A), 1:1000, rabbit polyclonal; and mNeonGreen (ChromoTek

(6) Role of oxidation of excitation-contraction coupling machinery in age-dependent loss of muscle function in Caenorhabditis eleganseLifeMay 4, 2022Haikel Dridi, Frances Forrester, Alisa Umanskaya, Wenjun Xie, Steven Reiken, Alain Lacampagne, Andrew MarksCruz,Cat. #: sc6104,"WB (1:2500), (10 μl)" Antibody,anti-FKBP12 (Rabbit polyclonal),Abcam,Cat. #: ab2918,"WB (1:2000), (10 μl)" Commercial assay or kit,Oxyblot protein oxydation detection kit,Millipore,Cat.

(7) Immunosuppressive calcineurin inhibitor cyclosporine A induces proapoptotic endoplasmic reticulum stress in renal tubular cellsThe Journal of biological chemistryMarch 1, 2022Yilmaz DE, Kirschner K, Demirci H, Himmerkus N, Bachmann S, Mutig K.polyclonal","1:1000, 5% BSA in PBS",ab41684 FKBP12,Abcam,"Rabbit, polyclonal","1:1000, 5% BSA in PBS",ab2918 GAPDH,Abcam,"Rabbit, polyclonal","1:2000, 5% BSA in TBS",ab181602 IRE1α,Cell Signaling Technology,"Rabbit,

(8) Aurora B phosphorylates Bub1 to promote spindle assembly checkpoint signalingCurrent biology : CBJanuary 10, 2022Roy B, Han SJY, Fontan AN, Jema S, Joglekar AP.α-Ds-Red,SantaCruz Biotechnologies,sc-390909 Mouse α-βTubulin,Millipore Sigma,T7816 Rabbit α-Fkbp12,Abcam,ab2918 Rabbit α-PhosphoMELT,48,N/A Mouse α-Cdc20,Santa Cruz Biotechnologies,sc-13162 Mouse α-Mad2,Millipore Sigma

(9) Modulating FKBP5/FKBP51 and autophagy lowers HTT (huntingtin) levelsAutophagyDecember 1, 2021Bailus BJ, Scheeler SM, Simons J, Sanchez MA, Tshilenge KT, Creus-Muncunill J, Naphade S, Lopez-Ramirez A, Zhang N, Lakshika Madushani K, et al.Fisher, 88,520). After blocking in 5% milk in TBST, primary antibody FKBP1A/FKBP12 (1:1000–4000; Abcam, ab2918), FKBP1B/FKBP12.6 (1:100–200, Abcam, ab82316); FKBP5/FKBP51 (1:100–500; Cell Signaling Technology, D5G2),

(10) Selective inhibitors of mTORC1 activate 4EBP1 and suppress tumor growthNature Chemical BiologyOctober 1, 2021Bianca J. Lee et al.USA); anti-Cyclin D1 (17H3L3) #701421 from Thermo Fisher Scientific (Waltham, MA, USA); anti-FKBP12 #ab2918 (Abcam, Cambridge, UK); anti-β-actin (Clone AC-15) #A1978 from Sigma (Saint Louis, Missouri, USA); anti-puromycin

(11) Loss of α-actinin-3 during human evolution provides superior cold resilience and muscle heat generationAmerican journal of human geneticsMarch 4, 2021Wyckelsma VL, Venckunas T, Houweling PJ, Schlittler M, Lauschke VM, Tiong CF, Wood HD, Ivarsson N, Paulauskas H, Eimantas N, et al.anti-RyR1 (1:5,000, mouse, ab2868, Abcam) and anti-12-kDa FK506 binding protein (FKBP12; 1:1,000 rabbit, ab2918, Abcam) antibodies. After washing with TBS-T, membranes were incubated in secondary antibody (1:20,000,

(12) ESCargo: a regulatable fluorescent secretory cargo for diverse model organismsMolecular Biology of the CellDecember 15, 2020Jason C. Casler, Allison L. Zajac, Fernando M. Valbuena, Daniela Sparvoli, Okunola Jeyifous, Aaron P. Turkewitz, Sally Horne-Badovinac, William N. Green, Benjamin S. Glickroom temperature for 1 h and then incubated with 1:1000 polyclonal rabbit anti-FKBP12 antibody (Abcam; ab2918) in 5% milk/TBST with shaking overnight at 4°C. After four 5-min washes in TBST, the membrane was incubated

(13) A microscopy-based kinetic analysis of yeast vacuolar protein sortingeLifeJune 25, 2020Jason C Casler, Benjamin S Glicktemperature for 1 hr, and then incubated with 1:1000 polyclonal rabbit anti-FKBP12 antibody (Abcam; ab2918) in 5% milk/TBST with shaking overnight at 4°C. After four 5 min washes in TBST, the membrane was incubated

(14) FK506 regulates Ca²⁺ release evoked by inositol 1,4,5-trisphosphate independently of FK-binding protein in endothelial cellsBritish journal of pharmacologyMarch 1, 2020Buckley C, Wilson C, McCarron JG.and incubated overnight at 4°C either with the primary antibody (Abcam rabbit polyclonal Anti‐FKBP12, Ab2918) or without it (for a no‐primary control) in antibody buffer (20× SSC, 2% donkey serum, 1% BSA, 0.05%

(15) Cardiac ryanodine receptor distribution is dynamic and changed by auxiliary proteins and post-translational modificationeLifeJanuary 9, 2020Asghari P, Scriven DR, Ng M, Panwar P, Chou KC, van Petegem F, Moore ED.Chemiluminescent Substrate kit (Thermo Scientific, Rockford, IL). We used the following antibodies: Anti-FKBP12 (ab2918; Abcam, Cambridge, UK) to detect both FKBP 12 and FKBP12.6, anti Calsequestrin (ab3516; Abcam) and anti-RyR2

(16) A novel rapamycin analog is highly selective for mTORC1 in vivoNature communicationsJuly 19, 2019Schreiber KH, Arriola Apelo SI, Yu D, Brinkman JA, Velarde MC, Syed FA, Liao CY, Baar EL, Carbajal KA, Sherman DS, et al.Cell Signaling Technology; βactin (A2228) was from Sigma; ATGL (SC-365278) was from Santa Cruz; and FKBP12 (ab2918) was from Abcam. All antibodies for western blotting were used at a dilution of 1:1000, except that

(17) Visualizing Secretory Cargo Transport in Budding YeastCurrent Protocols in Cell BiologyJune 1, 2019Jason C. Casler, Benjamin S. Glickbuffer containing β-mercaptoethanol Polyclonal rabbit anti-FKBP12 antibody (Abcam, product number ab2918) Goat anti-rabbit secondary antibody conjugated to Alexa Fluor 647 (Thermo Fisher, product number

(18) Maturation-driven transport and AP-1–dependent recycling of a secretory cargo in the GolgiJournal of Cell BiologyMay 6, 2019Jason C. Casler, Effrosyni Papanikou, Juan J. Barrero, Benjamin S. Glicktemperature for 1 h, and then incubated with 1:1,000 polyclonal rabbit anti-FKBP12 antibody (Abcam; ab2918, raised against an N-terminal peptide from FKBP) in 5% milk/TBST with shaking overnight at 4°C. After

(19) SR Ca²⁺ leak in skeletal muscle fibers acts as an intracellular signal to increase fatigue resistanceThe Journal of general physiologyApril 1, 2019Ivarsson N, Mattsson CM, Cheng AJ, Bruton JD, Ekblom B, Lanner JT, Westerblad H.Invitrogen), rabbit anti-PGC1α (ab54481; Abcam), rabbit anti-actin (ab1801; Abcam), rabbit anti-FKBP12 (ab2918; Abcam), mouse anti-DHPR(α 2 subunit; ab2864; Abcam), mouse anti-SERCA1 (ab2819; Abcam), and rabbit

(20) Atrial fibrillation and electrophysiology in transgenic mice with cardiac-restricted overexpression of FKBP12American Journal of Physiology-Heart and Circulatory PhysiologyFebruary 1, 2019Zhenwei Pan et al.Western blots were performed as previously described (17). Rabbit polyclonal FKBP12 antibody (catalog no. ab2918, 1:2,000) was from Abcam. Rabbit polyclonal Cav1.2 antibody (catalog no. C1241, 1:200) was from Sigma.

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5/4/2022Really Good FKBP12 Antibody for Western Blot Chang Seok LeeI used this Ab for detecting FKBP12 in the skeletal muscle of FKBP12KO and WT control mice. This Ab is really good for western blot.

Anti-FKBP12 antibody from Abcam

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Anti-FKBP12 antibody from Abcam

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