Fig 1: Comparison of HSP90 Inhibition Response in Resting and Activated T Cells, Related to Figure 7(A) Scatterplots showing protein fold changes (FC) observed in mature (left panel) and nascent (right panel) forms of proteins in anti-CD3/CD28-activated primary T cells treated for 24 h with 17-AAG. Red closed circles indicate statistically significant regulation (p < 0.05), proteins in rectangle are quantified against background noise and the dashed diagonal indicates the equality line. N indicates the number of proteins robustly quantified in both replicates.(B) Comparison of proteome HSP90 dependence between resting and anti-CD3/CD28-activated primary T cells at 24 h. Proteins are grouped as follow: not identified, not regulated (no), dependent on HSP90 during synthesis, or constitutively. The number of proteins overlapping the different groups is indicated in a matrix display.
Fig 2: Comparison of HSP90-Dependent Proteins in Resting and Activated Primary T Cells(A) Scatterplots comparing global effects of HSP90 inhibition on mature proteins in resting (left) and anti-CD3/CD28 activated (right) primary T cells. Protein fold changes of 17-AAG versus vehicle treated samples are shown for two donors (full graph in Figure S7A). Significantly regulated proteins are marked in red (p < 0.05); the dashed line is the equality line.(B) Line chart with markers of fold changes for mature forms of selected proteins in resting (blue) and activated T cells (red) upon treatment with 17-AAG relative to vehicle control.(C) Line chart with markers of fold changes determined for nascent heat shock family members in resting (blue) and activated T cells (red) upon treatment with 17-AAG relative to vehicle control.Triangles and circles indicate the two donors. Significance is indicated by an asterisk (p < 0.05) (B and C).See also Table S3.
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