Anti-GFP antibody ab5449 from Abcam

Product Specs
ItemAnti-GFP antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab5449
IsotypeIgG
Quantity50 µL
ClonalityPolyclonal
HostGoat
ApplicationsIP, WB
Conjugate/TagUnconjugated
Gene NameGFP
Antibody TypePrimary Antibody
Research AreaResearch Tools
TargetGFP

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) ROS anchor PAMPs-mediated extracellular HMGB1 self-association and its dimerization enhances pro-inflammatory signalingRedox biologyMarch 1, 2025Kwak MS, Han M, Lee YJ, Choi S, Kim J, Park IH, Shin JS.(Sigma Aldrich, P4707) in PBS at RT for 30 min, and after washing incubated with anti-GFP antibody (Abcam, ab5449) for 1 h. After blocking with 3% BSA-PBST, the supernatants were incubated at 4°C for 18 h. GFP fluorescence

(2) NLS-binding deficient Kapβ2 reduces neurotoxicity via selective interaction with C9orf72-ALS/FTD dipeptide repeatsCommun BiolJanuary 2, 2025Kevin M. Kim, Amandeep Girdhar, Maria E. Cicardi, Vaishnavi Kankate, Miyuki Hayashi, Ruoyu Yang, Jenny L. Carey, Charlotte M. Fare, James Shorter, Gino Cingolani, et al.Lysates were then incubated with magnetic beads that were pre-coupled with GFP antibody (Abcam, catalog#: ab5449). After overnight incubation, beads were washed twice after which the protein was eluted, boiled, and

(3) Knockdown of calpain1 in lumbar motoneurons reduces spasticity after spinal cord injury in adult ratsMolecular TherapyApril 3, 2024Marjorie Kerzonkuf, Jérémy Verneuil, Cécile Brocard, Nejada Dingu, Virginie Trouplin, Jose Jorge Ramirez Franco, Marc Bartoli, Frédéric Brocard, Hélène Brasperformed using a mixture of rabbit calpain1 antibody (1:200, 2556S, Ozyme) and goat GFP antibody (1:1,000, AB5449, Abcam). This labeling was preceded by the incubation of the slices in a methanol solution for 3 h before

(4) FOXL2 interaction with different binding partners regulates the dynamics of ovarian developmentSci AdvMarch 22, 2024Roberta Migale, Michelle Neumann, Richard Mitter, Mahmoud-Reza Rafiee, Sophie Wood, Jessica Olsen, Robin Lovell-Badge(Merck Millipore, catalog number AB5535), rat anti-GFP (Nacalai Tesque, 04404-84), goat anti-GFP (Abcam, ab5449), goat anti-FOXL2 (Abcam, ab5096), rabbit anti-FOXL2 (a gift from D. Wilhelm), rabbit anti-DDX4 (Abcam,

(5) Structural and Functional Insights into CP2c Transcription Factor ComplexesInternational Journal of Molecular SciencesJune 7, 2022Seung Han Son, Min Young Kim, Eunbi Jo, Vladimir N. Uversky, Chul Geun Kim(Abcam, ab131442; Santa cruz, sc-216), HA (Abcam, ab49969), Flag (Sigma-Aldrich, F1804), and EGFP (Abcam, ab5449). The blots were incubated for 1 h at room temperature with the following respective HRP-conjugated

(6) Intrinsic brainstem circuits comprised of Chx10-expressing neurons contribute to reticulospinal output in miceJournal of NeurophysiologyDecember 1, 2021Jeremy W. Chopek, Ying Zhang, Robert M. Brownstonefor immunohistochemistry. Sections were incubated with primary goat anti-GFP (1:500, Abcam, Cat. No. AB5449, RRID: AB_304896) antibodies diluted in PBS containing 0.1% Triton X-100 (PBS-T) for 24 h at 4°C. Sections

(7) Proneural genes define ground-state rules to regulate neurogenic patterning and cortical foldingNeuronSeptember 15, 2021Han S, Okawa S, Wilkinson GA, Ghazale H, Adnani L, Dixit R, Tavares L, Faisal I, Brooks MJ, Cortay V, et al.Thermo Fisher Scientific #13–0300), rabbit anti-GFP (1:500, Invitrogen #A-11122), goat anti-GFP (1:500, Abcam #ab5449), chicken anti-GFP (1:500, Abcam#ab13970), rabbit anti-Ki67 (1:500, Abcam #16667), rabbit anti-Laminin

(8) Reactive oxygen species induce Cys106-mediated anti-parallel HMGB1 dimerization that protects against DNA damageRedox BiologyApril 1, 2021Man Sup Kwak, Woo Joong Rhee, Yong Joon Lee, Hee Sue Kim, Young Hun Kim, Min Kyung Kwon, Jeon-Soo ShinRoskilde, Denmark) was passivated with methoxy polyethylene glycol, and 1 μg of anti-GFP antibody (Abcam, ab5449), which cross-reacts with YFP and CFP, was immobilized for 1 h followed by washing with 0.5% Tween 20

(9) Multimodal detection of protein isoforms and nucleic acids from mouse pre-implantation embryosNature protocolsFebruary 1, 2021Rosàs-Canyelles E, Modzelewski AJ, Geldert A, He L, Herr AE.(Millipore cat. no. AB5603, RRID: AB_2286686) and goat anti-green fluorescent protein (GFP) (Abcam, cat. no. ab5449, RRID: AB_304896). Example labeled primary antibodies include rhodamine anti-actin primary antibody

(10) Novel CAPN3 variant associated with an autosomal dominant calpainopathyNeuropathology and Applied NeurobiologyOctober 1, 2020M. Cerino et al.Western blot Western blot analysis was performed on protein extracts from transfected cells with anti-GFP antibody 1/5000 (ab5449, lot number GR178625-1; Abcam, Cambridge, UK) and anti-V5tag antibody 1/1000 (ab27671, lot number GR322548-6;

(11) Tropomyosin 1 genetically constrains in vitro hematopoiesisBMC biologyMay 14, 2020Thom CS, Jobaliya CD, Lorenz K, Maguire JA, Gagne A, Gadue P, French DL, Voight BF.(15D12.2, MAB2254, Millipore Sigma), Mouse anti-TPM3 (3D5AH3AB4, ab113692, Abcam), Rabbit anti-TPM4 (AB5449, Millipore Sigma), and Mouse anti-β Actin (A1978, Sigma). Western blot band quantitation was performed

(12) Loss of Cajal bodies in motor neurons from patients with novel mutations in VRK1Human molecular geneticsJuly 15, 2019El-Bazzal L, Rihan K, Bernard-Marissal N, Castro C, Chouery-Khoury E, Desvignes JP, Atkinson A, Bertaux K, Koussa S, Lévy N, et al.the cells, GFP signal was amplified by immunohistochemistry using a goat anti-GFP antibody (Abcam, ab5449) following incubation with an anti-goat secondary antibody (Abcam, ab96931). Fluorescence was observed

(13) Phosphorylation of nuclear Tau is modulated by distinct cellular pathwaysScientific reportsDecember 7, 2018Ulrich G, Salvadè A, Boersema P, Calì T, Foglieni C, Sola M, Picotti P, Papin S, Paganetti P.histone 3 (Abcam, ab176842, 1:10,000), β-actin (Sigma-Aldrich, A1978, 0.084 μg/mL), and GFP (Abcam, ab5449, 1:5,000). Samples preparation for LC-MS/MS Detergent-free extracts were prepared in PBS supplemented

(14) The effect of intrauterine growth restriction on Ca²⁺ -activated force and contractile protein expression in the mesenteric artery of adult (6-month-old) male and female Wistar-Kyoto ratsPhysiological reportsDecember 1, 2018Christie MJ, Romano T, Murphy RM, Posterino GS.polyclonal IgG; 1:10,000; ab5694; lot no. GR248336‐21; Abcam, UK) and TPM4 (rabbit polyclonal IgG; 1:1000; AB5449; lot no. 2742738; Merck Millipore, Germany); note: all antibodies were independently verified in our

(15) Variants in exons 5 and 6 of ACTB cause syndromic thrombocytopeniaNature communicationsOctober 12, 2018Latham SL, Ehmke N, Reinke PYA, Taft MH, Eicke D, Reindl T, Stenzel W, Lyons MJ, Friez MJ, Lee JA, et al.anti-α-actinin 1 (Merck Millipore, Darmstadt, Germany; #MAB1682), rabbit anti-TPM4 (Merck Millipore; #AB5449) and rabbit anti-DIAPH1 (Abcam Inc.; #Ab96784). Conjugated primary antibodies used for immunofluorescence

(16) Mbd2-CP2c loop drives adult-type globin gene expression and definitive erythropoiesisNucleic acids researchJune 1, 2018Kim MY, Kim JS, Son SH, Lim CS, Eum HY, Ha DH, Park MA, Baek EJ, Ryu BY, Kang HC, et al.the following antibodies: CP2c (Cosmogentec), CP2b (Peptron), Pias1 (Lifespan LS-C90260), EGFP (Abcam ab5449), HA (Abcam ab49969), Flag (Sigma Aldrich F1804), His (Santa Cruz sc-804), GST (Bodytech), Myc (Abcam

(17) Stress-induced release of Oct-1 from the nuclear envelope is mediated by JNK phosphorylation of lamin B1PloS oneJanuary 1, 2017Boubriak II, Malhas AN, Drozdz MM, Pytowski L, Vaux DJ.8D1) [11], antiOct1 (Santa Cruz; sc-232 and sc-232x), anti-Oct1 (GenTech; GTX105202) anti-GFP (Abcam; ab5449), anti-GFP (GeneTech; GT7312) anti-JNK (Cell Signalling; 9258), anti-phospho-JNK (Cell Signalling; 4668),

(18) Astrocyte Intermediaries of Septal Cholinergic Modulation in the HippocampusNeuronMay 18, 2016Milan Pabst, Oliver Braganza, Holger Dannenberg, Wen Hu, Leonie Pothmann, Jurij Rosen, Istvan Mody, Karen van Loo, Karl Deisseroth, Albert J Becker, Susanne Schoch, Heinz Beck

(19) Synergy of direct and indirect cholinergic septo-hippocampal pathways coordinates firing in hippocampal networksThe Journal of neuroscience : the official journal of the Society for NeuroscienceJune 3, 2015Dannenberg H, Pabst M, Braganza O, Schoch S, Niediek J, Bayraktar M, Mormann F, Beck H.and goat anti-GFP Dannenberg et al. • Cholinergic Septo-Hippocampal Pathways polyclonal antibody (ab5449; Abcam) diluted 1:1000 were used as first antibodies and Cy3-conjugated donkey anti-rabbit-IgG polyclonal

(20) In vivo generation of a mature and functional artificial skeletal muscleEMBO Molecular MedicineApril 1, 2015Claudia Fuoco et al.

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Anti-GFP antibody from Abcam

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Anti-GFP antibody from Abcam

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