Fig 1: ZMYM2-FGFR1 fusion protein is degraded by pomalidomide.a Schematic diagram of ZMYM family proteins. b In vitro interaction assay between CRBN and ZMYM2, ZMYM3 or ZMYM4. The interaction between bls-CRBN and FLAG-GST-ZMYM2, -ZMYM3 or -ZMYM4 in the presence of DMSO or 20 µM thalidomide (Th), lenalidomide (Le), pomalidomide (Po) or 5-hydroxythalidomide (5HT) was analysed using the AlphaScreen-based biochemical assay. All relative AlphaScreen (AS) signals were expressed as a luminescence signal relative to that of DMSO. Error bars denote the standard deviation (independent experiments; n = 3). c In vitro interaction assay between CRBN and ZMYM2-WT or ZMYM2-C470A. The interaction between bls-CRBN and FLAG-GST-ZMYM2-WT or -C470A in the presence of DMSO or 20 µM pomalidomide (Po) was analysed using the AlphaScreen-based biochemical assay. All relative AlphaScreen (AS) signals were expressed as a luminescence signal relative to that of DMSO. Error bars denote the standard deviation (independent experiments; n = 3). d Immunoblot analysis of ZMYM2-AGIA protein levels in HEK293T cells expressing ZMYM2-WT- or ZMYM2-C470A-AGIA and FLAG-CRBN treated with DMSO or pomalidomide (Po) for 16 h. The experiments were repeated three times independently with similar results. e Schematic diagram of ZMYM2, FGFR1, ZMYM2-FGFR1 protein. f In vitro interaction assay between CRBN and ZMYM2, FGFR1 or ZMYM2-FGFR1. The interaction between bls-CRBN and FLAG-GST-ZMYM2, -FGFR1 or -ZMYM2-FGFR1 in the presence of DMSO or 20 µM pomalidomide (Po) was analysed using the AlphaScreen-based biochemical assay. All relative AlphaScreen (AS) signals were expressed as a luminescence signal relative to that of DMSO. Error bars denote the standard deviation (independent experiments; n = 3). g Immunoblot analysis of protein levels in HEK293T cells stably expressing empty, ZMYM2-WT- FGFR1- or ZMYM2-C470A-AGIA treated with DMSO or pomalidomide (Po) every 24 h for 48 h. The experiments were repeated three times independently with similar results. h Immunoblot analysis of protein levels in HEK293T cells stably expressing empty, ZMYM2-FGFR1-WT-AGIA (WT) or ZMYM2-C470A-AGIA (C470A) treated with DMSO or pomalidomide (Po) every 24 h for 48 h. The experiments were repeated three times independently with similar results. Source data are provided as a Source data file.