Fig 2: The correlation between RAD51AP1 expression and immune filtration level in ESCC. A The correlation between infiltration level of immune cells and RAD51AP1 expression in ESCC. B–D RAD51AP1 expression was positively associated with the enrichment of Th2 cells, T helper cells, and Treg cells. E–G RAD51AP1 expression was negatively associated with the enrichment of Mast cells, Neutrophils cells and Th17 cells

Fig 3: Role of UAF1 and RAD51AP1 in FANCD2 deubiquitination in cells. a HeLa cells depleted for endogenous UAF1 and stably expressing UAF1 (WT), UAF13A (3A), or UAF111A (11A) were examined for their level of ubiquitinated FANCD2 by western blotting. Ku86 was included as the loading control. b Survival of cells (from a) upon treatment with MMC or olaparib. The error bars represent mean values±S.D. of data from three independent experiments. c RAD51AP1 knockout HeLa cells depleted for endogenous UAF1 and stably expressing WT or mutant UAF1 with or without the co-expression of either RAD51AP1 (AP1) or the RAD51AP1DM (DM) mutant were examined for their level of ubiquitinated FANCD2. Ku86 was included as the loading control. The error bars represent the mean+S.D. of data from three independent experiments. d Survival of cells (from c) upon treatment with MMC. The error bars represent mean values±S.D. of data from three independent experiments. e Model for the role of the USP1-UAF1-RAD51AP1 complex in the FA pathway. Mono-ubiquitination and deubiquitination of FANCD2 both occur on DNA. DNA binding by either UAF1 or RAD51AP1 is sufficient for targeting the trimeric DUB complex to DNA bound, mono-ubiquitinated FANCD2

Fig 4: Role of UAF1 and RAD51AP1 DNA binding in FANCD2 deubiquitination. a Deubiquitination of FANCD2 by USP1-UAF1 that harbored wild type or mutant UAF1. The error bars represent the mean+S.D. of data from three independent experiments. b Testing of RAD51AP1 and RAD51AP1DM in FANCD2 deubiquitination. The error bars represent the mean+S.D. of data from three independent experiments

Fig 5: Knockdown of RAD51AP1 suppressed cell migration and invasion of in ESCC. A Transwell assay showed that knockdown of RAD51AP1 significantly inhibited cell migration in KYSE150 and TE1 cells. B Transwell assay showed that knockdown of RAD51AP1 significantly inhibited cell invasion in KYSE150 and TE1 cells. ESCC, esophageal squamous cell carcinoma. *p < 0.05; **p < 0.01, ***p < 0.001