Anti-TRPC3 Antibody ACC-016 from Alomone Labs, Ltd.

Product Specs
ItemAnti-TRPC3 Antibody
CompanyAlomone Labs, Ltd.
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberACC-016
Brand/TrademarkAlomone Labs Ltd
HostRabbit
FormatReconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3., Lyophilized Powder
ReactivityHuman (Tested);Mouse (Tested);Rat (Tested)
Quantity1 X 50 mcl, 1 X 25 mcl, 1 X 0.2 ml
ApplicationsICC, IF, IFC, IHC, IP, WB
Conjugate/TagUnconjugated
ClonalityPolyclonal
Antibody TypePrimary
Concentration08 mg/ml
PurityAffinity purified on immobilized antigen
IsotypeIgG
ImmunogenSynthetic peptide
Domain/Region/TerminusIntracellular, C-terminus
Research AreaBreast cancer, Cancer, Cardiovascular, Hypertension, Pulmonary Hypertension, Vascular Hypertension, T-cells, t cells, t lymphocytes, immunology, immunity, immune cells
Gene NameTRPC3
TargetTRPC3 Channel
ValidationAs per applications, See images, contact for clarifications
pH RangepH 7.4

Specifications/Features

starting price for North American orders, for exact pricing view product page

Sequence

HKLSEKLNPSVLRC, corresponding to amino acid residues 822-835 of mouse TRPC3

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Alomone Labs, Ltd.

PO Box 4287

Jerusalem 91042

Israel

Phone: +972-2-587-2202
+972-2-587-1101

Company Profile

Website: http://www.alomone.com/

(1) Store-Operated Calcium Entry Increases Nuclear Calcium in Adult Rat Atrial and Ventricular CardiomyocytesCellsNovember 23, 2023Julia Hermes, Vesela Borisova, Jens Kockskämperrabbit anti-TRPC 1 (Alomone labs, Jerusalem, Israel, #ACC-010, 1:1000), rabbit anti-TRPC 3 (Alomone, #ACC-016, 1:1000), rabbit anti-TRPC 6 (Alomone, #ACC-017, 1:250), mouse anti-STIM1 (Thermo Scientific, #MA1-19451,

(2) A high-salt diet promotes hypertrophic scarring through TRPC3-mediated mitochondrial Ca2+ homeostasis dysfunctionHeliyonAugust 2, 2023Weijie Xia, Qianran Wang, Shaoyang Lin, Yuanyuan Wang, Junbo Zhang, Hailin Wang, Xia Yang, Yingru Hu, Huaping Liang, Yuangang Lu, et al.performed. The primary antibodies applied were anti-αSMA (1:150, Abcam, ab7817), anti-TRPC3 (1:200, Alomone, ACC-016), anti-CD31 (1:100, Abcam, ab222783) and anti-vimentin (1:200, Abcam, ab8978), and the secondary antibody

(3) Immunohistochemistry Reveals TRPC Channels in the Human Hearing Organ—A Novel CT-Guided Approach to the CochleaInternational Journal of Molecular SciencesMay 26, 2023Colya N. Englisch, Jakob Steinhäuser, Silke Wemmert, Martin Jung, Joshua Gawlitza, Gentiana Wenzel, Bernhard Schick, Thomas Tschernigagainst TRPC channels (TRPC3/5/6) and myosin antigen structures was applied (Alomone Labs; cat. no. ACC 016, 017, 020, Jerusalem, Israel, Anti-Myosin VIIa/MYO7A antibody, Abcam ab230631, Cambridge, UK). The specificity

(4) Lithium Treatment Improves Cardiac Dysfunction in Rats Deprived of Rapid Eye Movement SleepInternational Journal of Molecular SciencesSeptember 23, 2022Pao-Huan Chen, Cheng-Chih Chung, Shuen-Hsin Liu, Yu-Hsun Kao, Yi-Jen ChenCA, USA), TRPC 1 channel (1:2000, polyclonal, #ACC-010; Alomone Labs), TRPC3 (1:5000, polyclonal, #ACC-016; Alomone Labs), and TRPC6 (1:2000, polyclonal, #ACC-017; Alomone Labs). Bound antibodies were detected

(5) Induction of reactive oxygen species by mechanical stretch drives endothelin production in neonatal pig renal epithelial cellsRedox biologySeptember 1, 2022Kumar R, Soni H, Afolabi JM, Kanthakumar P, Mankuzhy PD, Iwhiwhu SA, Adebiyi A.rabbit monoclonal anti-NOX4 (Abcam; ab133303), rabbit polyclonal anti-TRPC3 (Alomone, Jerusalem Israel; ACC-016), rabbit polyclonal anti-ET receptor A (Alomone; AER-001), rabbit polyclonal anti-ET receptor B (Bioss

(6) TRPC6 Inactivation Reduces Albuminuria Induced by Protein Overload in Sprague Dawley RatsCellsJune 21, 2022Kim EY, Dryer SE.methods were previously described in detail [18]. Rabbit antibodies against TRPC6 (ACC-017) and TRPC3 (ACC-016) were obtained from Alomone Labs (Jerusalem, Israel). A mouse monoclonal antibody against TRPC5 was

(7) TRPC3 shapes the ER-mitochondria Ca²⁺ transfer characterizing tumour-promoting senescenceNature communicationsFebruary 17, 2022Farfariello V, Gordienko DV, Mesilmany L, Touil Y, Germain E, Fliniaux I, Desruelles E, Gkika D, Roudbaraki M, Shapovalov G, et al.incubated with the following primary antibodies: rabbit polyclonal anti-TRPC3 (1/200) Alomone Labs Cat# ACC-016; mouse monoclonal anti-GAPDH clone 6C5 (1/2000) Abcam Cat# ab8245; mouse monoclonal anti-Beta-Actin

(8) Lipin-1-derived diacylglycerol activates intracellular TRPC3 which is critical for inflammatory signalingCellular and molecular life sciences : CMLSDecember 1, 2021Casas J, Meana C, López-López JR, Balsinde J, Balboa MA.

(9) Calsequestrin 1 Is an Active Partner of Stromal Interaction Molecule 2 in Skeletal MuscleCellsOctober 20, 2021Jeong SY, Oh MR, Choi JH, Woo JS, Lee EH.

(10) OIP5-AS1 specifies p53-driven POX transcription regulated by TRPC6 in gliomaJournal of Molecular Cell BiologySeptember 11, 2021Wei Shao et al.Reagent,Resource,Identifier Antibodies,Antibodies,Antibodies POX antibody,Abcam,"ab203875, ab93210" TRPC3 antibody,Alomone,ACC-016 p53 antibody,Santa Cruz,sc-126 p21 antibody,Santa Cruz,sc-6246 TRPC6 antibody,Alomone,ACC-017 PTEN

(11) TRPC3 and NALCN channels drive pacemaking in substantia nigra dopaminergic neuronseLifeAugust 19, 2021Um KB, Hahn S, Kim SW, Lee YJ, Birnbaumer L, Kim HJ, Park MK.(1:500, Millipore, USA, MAB318, RRID: AB_2201528) and rabbit-anti-TRPC3 (1:500, Alomone lab, Israel, ACC-016, RRID: AB_2040236). After the primary antibody reaction, tissues were washed three times with NGS in

(12) Doxorubicin-Induced Fetal Mesangial Cell Death Occurs Independently of TRPC6 Channel Upregulation but Involves Mitochondrial Generation of Reactive Oxygen SpeciesInternational journal of molecular sciencesJuly 15, 2021Matthews AT, Soni H, Robinson-Freeman KE, John TA, Buddington RK, Adebiyi A.

(13) Gastrointestinal motility modulation by stress is associated with reduced smooth muscle contraction through specific transient receptor potential channelThe Journal of veterinary medical scienceApril 9, 2021Azuma YT, Suzuki S, Nishiyama K, Yamaguchi T.previously described method [5] 19 with some modifications [30]. Rabbit polyclonal anti-TRPC3 antibody (#ACC-016) was 20 used from Alomone Labs (Jerusalem, Israel). -actin was used as an endogenous control. 21 22 Recording

(14) Aging increases senescence, calcium signaling, and extracellular matrix deposition in human airway smooth musclePloS oneJanuary 1, 2021Wicher SA, Roos BB, Teske JJ, Fang YH, Pabelick C, Prakash YS.NBP1-06039), Orai1 (Alomone ACC-060), SERCA1 (Alomone ACP-011), SERCA2 (Alomone ACP-012), TRPC3 (Alomone ACC-016) STIM1 abcam ab62031) and STIM2 (Novus NBP1-76790). Protein expression was normalized to GAPDH (Cell

(15) Mechanosensitivity is an essential component of phototransduction in vertebrate rodsPLoS biologyJuly 1, 2020Bocchero U, Falleroni F, Mortal S, Li Y, Cojoc D, Lamb T, Torre V.

(16) Estrogen enhances the proliferation and migration of ovarian cancer cells by activating transient receptor potential channel C3Journal of ovarian researchFebruary 22, 2020Li S, Jiang K, Li J, Hao X, Chu W, Luo C, Zhu Y, Xie R, Chen B.After blocking with 5% milk in TBST for 2 h, the membranes were incubated with an anti-TRPC3 (Alomone, ACC016, Jerusalem, Israel) or anti-β-actin (CST, PA5–85271, Massachusetts, USA) antibody overnight at 4 °C.

(17) Vascular smooth muscle TRPC3 channels facilitate the inverse hemodynamic response during status epilepticusScientific reportsJanuary 21, 2020Cozart MA, Phelan KD, Wu H, Mu S, Birnbaumer L, Rusch NJ, Zheng F.RRID:AB_2336834) and a knock out-validated rabbit anti-mouse TRPC3 polyclonal antibody (Alomone Labs Cat# ACC016, Lot# ACC016AG1240, RRID:AB_2040236)32 was used at 1:400 dilution with goat anti-rabbit Alexa 647-conjugated

(18) Direct Activation of TRPC3 Channels by the Antimalarial Agent ArtemisininCellsJanuary 14, 2020Urban N, Schaefer M.for one hour. The primary antibodies, polyclonal rabbit anti-TRPC3 (Alomone Labs, Jerusalem, Israel; ACC-016; 1:100) and monoclonal mouse anti-tyrosine hydroxylase (Merck, Darmstadt, Germany; MAB 5280; 1:800),

(19) lncRNA DLX6-AS1 Promotes Proliferation of Laryngeal Cancer Cells by Targeting the miR-26a/TRPC3 PathwayCancer management and researchJanuary 1, 2020Liu Y, Liu X, Zhang X, Deng J, Zhang J, Xing H.

(20) Transient Receptor Potential Channel 6 Knockout Ameliorates Kidney Fibrosis by Inhibition of Epithelial-Mesenchymal TransitionFrontiers in cell and developmental biologyJanuary 1, 2020Zhang Y, Yin N, Sun A, Wu Q, Hu W, Hou X, Zeng X, Zhu M, Liao Y.(Proteintech, Chicago, Illinois, USA), anti-TRPC6 (Alomone, Jerusalem, Israel), anti-TRPC3 (Alomone:ACC-016), anti-mouse IgG (KeRui, Wuhan, Hubei, China), anti-rabbit IgG antibody (KeRui, Wuhan, Hubei, China),

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