Fig 1: TLs from Trp53+/− Nucks1+/− mice infiltrate distant organs more frequently than TLs from Trp53+/− miceTL cells infiltrating target organs were surrounded by blood vessels and were discriminated from the target tissue based on H&E staining (left). Further evidence of infiltration was obtained by IHC using anti-CD3 (TL-122 and TL-65), anti-TdT (TL-122) and anti-NUCKS1 (TL-157) antibodies. TL-122: Trp53+/−; TL-65 and TL-157: Trp53+/− Nucks1+/−.
Fig 2: CD3 staining of the dermisImmunohistochemical analyses of inflammation using CD3 as a biomarker showed that there was a significantly smaller extent of inflammation in irradiated acellular dermal matrices (ADMs) than in non-irradiated ADMs. (A) CD3 positive cells in irradiated and non-irradiated ADMs. (B) Quantification of CD3. a)P<0.05.
Fig 3: Change of immune cell distribution after SARS-CoV-2 infection in the lungs and peripheral blood. (A–C) Summary of the percentage of lung-infiltrated immune cells in K18-hACE2 (A), SFTPB-hACE2 (B), and SCGB1A1-hACE2 (C) mice. Immunohistochemistry was performed to identify the distribution of immune cells in the lungs with anti-PTPRC, CD3, neutrophil, and F4/80 antibodies at 0, 2, 5, and 7 dpi. DAB-positive cells were counted using QuPath and analysed from three randomly selected images. (D–F) White blood cell counts in K18-hACE2 (D), SFTPB-hACE2 (E), and SCGB1A1-hACE2 (F) mice from the peripheral blood at each time point post-infection.
Fig 4: Ebosin inhibits CD3+ and CD8+ T cell infiltration and epidermal hyperplasia in skin of psoriatic mice (scale bar is 50 μm). (A) Immunohistochemical images of CD3 staining (magnification: 100×) of dorsal skin and the relative IOD of control (mean ± SD, n = 3). (B) Immunohistochemical images of CD8 staining (magnification: 100×) of dorsal skin and the relative IOD of control (mean ± SD, n = 3). (C) Immunohistochemical images of Ki67 staining (magnification: 100×) of dorsal skin and the relative IOD of control (mean ± SD, n = 3). ## P < 0.01 vs. control; * P < 0.05, **P < 0.01 vs. IMQ-induced vehicle group.
Fig 5: Transcellular Pathway Alterations in The brain endothelium of Slc20a2-HO mice. (A) Expression of endocytosis- and transcytosis-related proteins in brain microvessels of 3.5-month-old Slc20a2-WT and -HO mice. **p < 0.01; *p < 0.05; n.s., not statistically significant. (B) Distribution pattern (localization, morphology, and polarization) of total caveolin-1 in 1-month-old mice. (C) The fusion of endothelial and lymphocyte cell membranes (red arrows) in 6-month-old -HO mice (C, Left). CD3+ colloidal gold particles in the endothelial cell membrane of the midbrain in 5-month-old -HO mice. Black triangles indicate gold particles (C, Right). EC, endothelial cell; L, vascular lumen. Scale bars, 6 and 1.5 μm (C, left); 0.5 μm (C, right). (D) Endogenous IgG and albumin, rather than fibronectin, leaked into the brains of 1-month-old -HO mice. Scale bar, 40 μm (B,D).
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