Fig 2: The distribution of TRIM28 protein in ESCC and NEE tissues by immunofluorescence stainings, ×400. (TRIM28 protein was stained with green fluorescence(→), which was expressed in the nucleus in ESCC cells and nuclei were counterstained with DAPI (A); Analysis results of the fluorescence intensity (B), P = 0.000) (One-way ANOVA)

Fig 3: Relative expression of TRIM28 protein by Western blot between paired ESCC tissues and NEE tissues (Cells in ESCC can express TRIM28 while there was no expression of TRIM28 in cells of NEE)

Fig 4: The expression of TRIM28 in ESCC and noncancerous tissues (Chi-square and Fisher’s exact tests)

Fig 5: Effects of GRh2 on (A) LPS-induced antioxidative enzymes (catalase, superoxide dismutase (SOD), and glutathione peroxidase (GPx)); heme oxygenase-1 (HO-1), Trx-1, NF-E2-related factor 2 (Nrf2)/Keap1 and KAP1; (B) TLR4, PI3K, Akt, and mTOR; and (C) Raf-1, p-Raf-1, Mek and p-Mek protein expression in the lungs. Protein levels of catalase, SOD, GPx, HO-1, Trx-1, Nrf2/Keap1, KAP1, TLR4, PI3K, Akt, mTOR, Raf-1, p-Raf-1, Mek and p-Mek protein expression in lung homogenates were evaluated by western blot analysis after LPS challenge 6 h hours later. Densitometric analysis of the relevant bands was performed. Data are represented as mean ± S.E.M. (n = 2). ## p < 0.05 and ### p < 0.001 compared with sample of control group. ** p < 0.01 and *** p < 0.001 compared with LPS-only group.