Anti-Hsp70 antibody [5A5] ab2787 from Abcam

Product Specs
ItemAnti-Hsp70 antibody [5A5]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab2787
Antibody TypePrimary
ClonalityMonoclonal
HostMouse, Nude mouse
Clone5A5
ApplicationsEMSA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot
Quantity250 UL
IsotypeIgG1
Conjugate/TagUnconjugated
Concentration9.3 mg/ml
Gene NameHSPA1A
ReactivityAfrican green monkey, Dog, Human, Mouse, Rat
TargetHsp70

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Circulating small extracellular vesicles microRNAs plus CA-125 for treatment stratification in advanced ovarian cancerJ Transl MedDecember 22, 2023Xiaofang Zhou, Mu Liu, Lijuan Sun, Yumei Cao, Shanmei Tan, Guangxia Luo, Tingting Liu, Ying Yao, Wangli Xiao, Ziqing Wan, et al.(Abcam, ab92726), CD63 (Abcam, ab216130), CD81 (Abcam, ab109201), TSG101 (Abcam, ab125011), HSP70 (Abcam, ab2787), calnexin (Abcam, ab22595), EpCAM (Abcam, ab32392), FAP (Abcam, ab207178), CD31 (Abcam, ab9498), CD45

(2) Unraveling Cartilage Degeneration through Synergistic Effects of Hydrostatic Pressure and Biomimetic Temperature IncreaseiScienceDecember 15, 2023Yanheng Guo, Theofanis Stampoultzis, Naser Nasrollahzadeh, Peyman Karami, Vijay Kumar Rana, Lee Applegate, Dominique P. Piolettifat-free milk in PBS-Tween (Sigma, US) and incubated with mouse anti-human HSP-70 antibody (1:250, ab2787, Abcam, UK) and mouse anti-β-actin antibody (1:200, A2228, Sigma, US) at 4°C overnight. Membranes were

(3) Optogenetic-mediated induction and monitoring of α-synuclein aggregation in cellular models of Parkinson’s diseaseSTAR ProtocolsDecember 1, 2023Maxime Teixeira, Razan Sheta, Walid Idi, Abid Oueslatimonoclonal; 1:5,000 (WB)",Applied Biological Materials,G043 "Mouse, anti-Hsp70 (5A5); 1:50 (ICC)",Abcam,ab2787 "Mouse, anti-SQSTM1 (p62); 1:1,000 (ICC)",Santa Cruz,Sc-28359 "Mouse, anti-ubiquitin monoclonal (Ubi-1);

(4) Hypoxic BMSC-derived exosomal miR-652-3p promotes proliferation and metastasis of hepatocarcinoma cancer cells via targeting TNRC6AAging (Albany NY)November 16, 2023Mei Li, Pengtao Zhai, Xudong Mu, Juanrong Song, Huilin Zhang, Juan Suroom temperature for 1h and incubated with indicated anti-CD63 (#ab271286; Abcam, USA), anti-HSP70 (#ab2787; Abcam, USA), anti-TSG101 (#ab125011; Abcam, USA), anti-Calnexin (#ab133615; Abcam, USA), anti-TNRC6A

(5) A novel technique to harvest bone autografts with mild local hyperthermia and enhanced osteogenic bone quality: a preclinical study in dogsBMC Oral HealthNovember 7, 2023Tengfei Zhou, Zekun Gan, Hanfei Zhang, Ziyi Liu, Yiping Pu, Mingdeng Rongmanufacturer’s instructions. Antibodies included Anti-GAPDH (1:10000, ab8245, Abcam), Anti-Hsp70 (1:1000, ab2787, Abcam), and HRP Conjugated AffiniPure Goat Anti-mouse IgG (H + L) (1:10000, BA1051, Boster). Protein

(6) Increased Expression of Tetraspanins, ALIX and HSP-70 in the Placenta Tissue of Women with Chronic Venous Disease during PregnancyInternational Journal of Medical SciencesOctober 16, 2023Miguel A Ortega, María Asunción Sánchez-Gil, Oscar Fraile-Martínez, Cielo García-Montero, Luis G Guijarro, Raúl Diaz-Pedrero, Laura Lopez-Gonzalez, Juan De Leon-Luis, Coral Bravo, Melchor Álvarez-Mon, et al.polyclonal,1:100,Abcam (ab76608),EDTA pH=9 before incubation with blocking solution HSP-70,Mouse monoclonal,1:100,Abcam(ab2787),EDTA pH=9 before incubation with blocking solution Secondary antibodies,,,, Rabbit IgG,Mouse,1:1000,Sigma-Aldrich(RG-96/B5283),------ Mouse

(7) The potential roles of stress‐induced phosphoprotein 1 and connexin 43 in rats with reperfusion arrhythmiaImmunity, Inflammation and DiseaseOctober 3, 2023Li An, Hong Gao, Yi Zhong, Yanqiu Liu, Ying Cao, Jing Yi, Xiang Huang, Chunlei Wen, Rui Tong, Zhijun Pan, et al.TritonX100, and incubated overnight with rabbit anti‐rat Cx43 (ab235282, Abcam) plus mouse anti‐rat HSP70 (ab2787, Abcam) or anti‐Cx43 plus mouse anti‐rat HSP90 (ab59459, Abcam). Later, the cells were incubated (in

(8) Exosomal NOX1 promotes tumor-associated macrophage M2 polarization-mediated cancer progression by stimulating ROS production in cervical cancer: a preliminary studyEuropean journal of medical researchSeptember 7, 2023Gu L, Feng C, Li M, Hong Z, Di W, Qiu L.skimmed milk for 2 h. Membranes were incubated overnight with primary antibodies against HSP70 (1:2000; ab2787, Abcam, USA), TSG101 (1:2000; 28283-1-AP, Proteintech, China), NOX1 (1:1500; 17772-1-AP, Proteintech,

(9) LncRNA KCNQ10T1 shuttled by bone marrow mesenchymal stem cell-derived exosome inhibits sepsis via regulation of miR-154-3p/RNF19A axisCell and Tissue ResearchSeptember 1, 2023Haojie Yuan, Junbo Yu, Chun Liu, Heyan Zhao, Jianhua Xue, Jiajia Liu, Yang Yangwith primary antibodies: CD63 (1:1000, ab134045, Abcam), CD9 (1:1000, ab92726, Abcam), HSP70 (1:1000, ab2787, Abcam), and VEGF (1:1000, ab32152, Abcam) at 4 °C overnight. On the following day, the membrane was

(10) Protocol to identify human subcellular alternative protein interactions using cross-linking mass spectrometrySTAR ProtocolsSeptember 1, 2023Diego Fernando Garcia-del Rio, Isabelle Fournier, Tristan Cardon, Michel Salzet,Santa Cruz Biotechnology,Cat# sc-517576; RRID: AB_2848194 Monoclonal Mouse anti-Hsp70 (1/1000),Abcam,Cat# ab2787; RRID: AB_303300 Monoclonal Mouse anti-SP1 (1/200),Santa Cruz Biotechnology,Cat# sc-420; RRID: AB_628271 Peroxidase AffiniPure Goat Anti-Mouse IgG (H + L

(11) MATN3 delivered by exosome from synovial mesenchymal stem cells relieves knee osteoarthritis: Evidence from in vitro and in vivo studiesJournal of Orthopaedic TranslationAugust 11, 2023Long Long, Guoyou Zou, Yi Cheng, Feng Li, Hao Wu, Yixin Shenwere identified by Western blot with the help of CD63 (ab216130, 1:1000, Abcam, Cambridge, UK), HSP70 (ab2787, 1:1000, Abcam), and endoplasmic reticulum marker protein Calnexin (ab22595, 1:1000, Abcam). 2.5 Isolation

(12) Influence of 12-Week Concurrent Training on Exosome Cargo and Its Relationship with Cardiometabolic Health Parameters in Men with ObesityNutrientsJuly 7, 2023Brisamar Estébanez, Francisco J. Amaro-Gahete, Cristina Gil-González, Javier González-Gallego, María J. Cuevas, David Jiménez-Pavónsc-5570) were purchased from Santa Cruz Biotechnology, Dallas, TX, USA; LAMP2A (Ref. ab18528), HSP70 (Ref. ab2787) and HSP60 (Ref. ab1428) were purchased from Abcam®, Cambridge, UK. The bound primary antibody was detected

(13) Termination of STING responses is mediated via ESCRT‐dependent degradationThe EMBO JournalJune 15, 2023Katherine R Balka, Rajan Venkatraman, Tahnee L Saunders, Angus Shoppee, Ee Shan Pang, Zoe Magill, Jihane Homman‐Ludiye, Cheng Huang, Rachael M Lane, Harrison M York, et al.include the following: β‐actin Mouse mAb HRP‐conjugated AC‐15 Abcam ab49900, Hsp70 Mouse mAb 5A5 Abcam ab2787. Membranes were then washed 3× in TBST (10–20 min per wash) and incubated with appropriate HRP‐conjugated

(14) Oxidative stress and inflammation distinctly drive molecular mechanisms of diastolic dysfunction and remodeling in female and male heart failure with preserved ejection fraction ratsFrontiers in Cardiovascular MedicineJune 8, 2023Saltanat Zhazykbayeva, Roua Hassoun, Melissa Herwig, Heidi Budde, Árpád Kovács, Hans Georg Mannherz, Ibrahim El-Battrawy, Attila Tóth, Wolfgang E. Schmidt, Andreas Mügge, et al.p10,Sc-7885,Santa Cruz Biotechnology,"1:1,000" HSP 27 (rodent preferred),2442S,Cell Signaling,"1:1,000" HSP 70,Ab2787,Abcam,"1:1,000" IL 6,P620,Invitrogen,"1:1,000" IL 18,PA5-80719,Invitrogen,"1:1,000" LC 3 A/B,12741S,Cell Signaling

(15) Muscle-derived stem cell exosomes with overexpressed miR-214 promote the regeneration and repair of rat sciatic nerve after crush injury to activate the JAK2/STAT3 pathway by targeting PTENFrontiers in Molecular NeuroscienceMay 26, 2023Xiangyu Zeng, Wei Bian, Ziwen Liu, Jianming Li, Shuai Ren, Jian Zhang, Haoran Zhang, Bu Tegeleqi, Guanyi He, Mingyan Guan, et al.temperature, followed by overnight incubation with primary antibodies anti-Hsp70 antibody (1:1000, ab2787, Abcam, United Kingdom), anti-CD63 antibody (1:1000, PA5-92370, Invitrogen, United States), anti-CD9

(16) Extracellular Vesicles Secreted by TGF-β1-Treated Mesenchymal Stem Cells Promote Fracture Healing by SCD1-Regulated Transference of LRP5Stem Cells InternationalMarch 15, 2023Zihui Zhou, Chenyang Guo, Xulong Sun, Zhengwei Ren, Jie Tao1 : 1,000), anti-CD81 (Abcam, ab286173, 1 : 500), anti-CD63 (Abcam, ab1318, 1 : 1,000), anti-HSP70 (Abcam, ab2787, 1 : 1,000), anti-β-actin (Abcam, ab7817, 1 : 2,000), anti-SREBP-1 (Abcam, ab28481, 1 : 1,000), anti-LRP5

(17) Sublethal Hyperthermia Transiently Disrupts Cortisol Steroidogenesis in Adrenocortical CellsEndocrinologyMarch 13, 2023Mullen N, Donlon PT, Sebek J, Duffy K, Cappiello G, Feely S, Warde KM, Harhen B, Finn DP, O'Shea PM, et al.Gomez-Sanchez and used at a dilution of 1:800 and 1:200 dilutions, respectively. HSP70 (Abcam Cat# ab2787, RRID:AB_303300) and HSP90 (Novus Cat# NB120-5455, RRID:AB_790276) antibodies were used with dilutions

(18) ARF suppression by MYC but not MYCN confers increased malignancy of aggressive pediatric brain tumorsNature communicationsMarch 3, 2023Mainwaring OJ, Weishaupt H, Zhao M, Rosén G, Borgenvik A, Breinschmid L, Verbaan AD, Richardson S, Thompson D, Clifford SC, et al.(1:1000)), GFP (Abcam, Cat # ab13970 (1:1000)), HSF1 (Abcam, Cat # ab61382 (1:1000)), HSP70 (Abcam, Cat # ab2787 (1:1000)), HSP90 [EPR16621] (Abcam, Cat # ab203085 (1:1000)), Ki67 [SP6] (Abcam, Cat # ab16667 (1:2000)),

(19) Synovial fibroblast-miR-214-3p-derived exosomes inhibit inflammation and degeneration of cartilage tissues of osteoarthritis ratsMolecular and Cellular BiochemistryMarch 1, 2023Chenteng Lai, Boyi Liao, Song Peng, Peng Fang, Nirong Bao, Lei Zhang(ab6671), CD81 (1:1000) (ab109201), CD63 (1:1000) (ab231975), CD9 (1:1000) (ab223052) and HSP70 (1:1000) (ab2787), all the antibodies were obtained from Abcam USA. The blots were detected using Western blot detection

(20) Employing non-targeted interactomics approach and subcellular fractionation to increase our understanding of the ghost proteomeiScienceFebruary 17, 2023Diego Fernando Garcia-del Rio, Tristan Cardon, Sven Eyckerman, Isabelle Fournier, Amelie Bonnefond, Kris Gevaert, Michel Salzet(Santa Cruz Biotechnology, sc-420), Histone H3 (Santa Cruz Biotechnology, sc-517576), Hsp70 (Abcam, ab2787), and Calreticulin (Abcam, ab2908). The matched HRP Anti-Rabbit (Abcam, ab6721) and Anti-Mouse (Jackson Immuno

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3/13/2018Good Antibody For HSP70 Detection Shikha SainiHSP70 is a member of a family of the proteins called HSP70 family. In co-operation with other proteins, it stabilizes proteins and prevents aggregation in the cytosol and other organelles. We ... Read Review

Anti-Hsp70 antibody [5A5] from Abcam

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Anti-Hsp70 antibody [5A5] from Abcam

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