Fig 1: Impact of low level of Calr in the Calr+/− mouse kidney on the expression of ER stress markers and EF-hand Ca2+-binding proteins. (A): Illustration of the experimental design for immunohistology and Western blot. (B,C): Western blot analyses and immunohistostaining of the ER stress markers Grp78, Erp57, Canx, Chop, and p-eIF-2A showed no significant difference between the Calr+/+ and Calr+/− mouse kidneys; Actb was used as loading control. Below: Bar diagram represents the quantification of the Western blot results shown in (B) (n = 4. *: p < 0.05). (D,E): Western blot analyses and immunohistostaining of some ER-hand Ca2+-binding proteins Pvalb, Calb1, Calm1, and S100A4 showed significant up-regulation in Calr+/− kidney compared to Calr+/+. Below: Bar diagram represents the quantification of the Western blot results shown in (D) (n = 4. *: p < 0.05, **: p < 0.01, ***: p < 0.001). The immunohistochemical staining of Grp78, Pvalb, Calm1, and Canx is shown in 40 wk old Calr+/− and Calr+/+ mouse kidney sections.