Fig 2: Association of CD46 with GP96 during HHV-6A infection.(A) Cell surface expression dynamics of GP96 in CHO-K1 cells stably expressing 55 kDa isoform of CD46. CHO-K1(5.3) cells expressing the 55 kDa isoform of CD46 were infected with HHV-6A for indicated time points. CD46 and GP96 cell surface expression were analyzed by flow cytometry (solid profiles); background fluorescence levels were measured using an isotype specific antibody (empty profiles). (B) Cell surface expression dynamics of GP96 in CHO-K1 cells stably expressing 65 kDa isoform of CD46. CHO-K1(5.1) cells expressing the 65 kDa isoform of CD46 were infected with HHV-6A for indicated time points. CD46 and GP96 cell surface expression were analyzed by flow cytometry (solid profiles); background fluorescence levels were measured using an isotype specific antibody (empty profiles). Mean fluorescence intensity (MFI) for each analysis is indicated below the respective figure.

Fig 3: Basal Cell Markers and Ad Receptor Expression in Primary Human Airway Basal Cells(A and B) Immunohistochemistry (A) or flow cytometry (B) was used to visualize cytokeratin (CK5) in primary basal cells. Scale bar, 50 µm. (C) Flow cytometry was used to quantify CAR, DSG2, and CD46 in primary basal cells.

Fig 4: Cell surface expression of GP96 is modulated during HHV-6 infection.Cell surface expression dynamics of GP96 and CD46 in response to HHV-6A infection was determined in (A) HeLa and (B) HSB-2 cells. Cells were infected with HHV-6A for indicated time points and cell surface expression of CD46 and GP96 was performed by flow cytometry (solid profiles); background fluorescence levels were measured using an isotype specific antibody (empty profiles). Mean fluorescence intensity (MFI) for each analysis is indicated below the respective figure.