Anti-BrdU antibody - Proliferation Marker ab1893 from Abcam

Product Specs
ItemAnti-BrdU antibody - Proliferation Marker
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab1893
Antibody TypePrimary
IsotypeIgG
Quantity250 UG
ApplicationsELISA, Immunohistochemistry - Free Floating, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry (Frozen sections), Western blot
HostSheep
ClonalityPolyclonal
Conjugate/TagUnconjugated
Concentration1.3 mg/ml
ReactivitySpecies independent
TargetBrdU

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Vaccination prevents IL-1β-mediated cognitive deficits after COVID-19Research SquareNovember 22, 2023Abigail Vanderheiden, Abigail Vanderheiden, Xiaoping Jiang, Xiaoping Deppen, Benjamin Bamunuarachchi, Gayan Bamunuarachchi, Nadia Soudani, Matthew D. Joshi, Adrianus C.M. Cain, Adrianus Kleinwere used: NeuN (1:200; Cell Signaling, cat no. 12943S, clone D3S3I), BrdU (1:250; Abcam, cat. no. ab1893, polyclonal), doublecortin (1:200; Cell Signaling, cat. no. 4604S, polyclonal), GFAP (1:200; Thermo,

(2) Repeated mild traumatic brain injury causes sex-specific increases in cell proliferation and inflammation in juvenile ratsJournal of NeuroinflammationOctober 31, 2023Katie J. Neale, Hannah M. O. Reid, Barbara Sousa, Erin McDonagh, Jamie Morrison, Sandy Shultz, Eric Eyolfson, Brian R. Christie(DAB),Abcam,Ab15580,AB_443209,1:1000 (5%),48,Goat anti Rabbit (Millipore Sigma B8895 Sheep anti-BrdU (IF),Abcam,Ab1893,AB_302659,1:1000,16,Goat anti Sheep Biotin (Invitrogen A24565) ,,,,,,Streptavidin Alexa Fluor 488 conjugate

(3) Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hostsOpen Veterinary JournalSeptember 30, 2023Theerayut Thongrin, Watcharapol Suyapoh, WoroDanur Wendo, Prasarn Tangkawattana, Peerapol Sukon, Kanin Salao, Sutas Suttiprapa, Prasert Saichua, Sirikachorn Tangkawatanaprimary and secondary antibodies used were polyclonal sheep anti-Bromodeoxyuridine (dilution 1:500, ab1893, Abcam plc, Cambridge, UK), at room temperature overnight. Then, the sections were incubated by biotinylated

(4) Spatiotemporal Regulation of De Novo and Salvage Purine Synthesis during Brain DevelopmenteNeuroSeptember 28, 2023Tomoya Mizukoshi, Seiya Yamada, Shin-Ichi Sakakibarapolyclonal, #GTX85457, RRID: AB_10627357, Gene Tex; 1:1500 for ICC), anti-BrdU (sheep polyclonal, #ab1893, RRID: AB_302659, Abcam, 1:1500 for IHC and ICC), anti-Ki67 (rabbit monoclonal clone SP6, #RM-9106,

(5) DOT1L deletion impairs the development of cortical parvalbumin-expressing interneuronsCerebral CortexSeptember 26, 2023Arquimedes Cheffer, Marta Garcia-Miralles, Esther Maier, Ipek Akol, Henriette Franz, Vandana Shree Vedartham Srinivasan, Tanja VogelCell Signaling; 9664S), anti-GFP (chicken; 1:500; Abcam; ab13970), anti-BrdU (sheep; 1/100; Abcam; ab1893), anti-Ki67 (rabbit; 1:250; Abcam; ab15580), anti-SOX6 (rabbit; 1:50; Santa Cruz; sc-20,092), anti-PHH3

(6) Adult-born neurons in critical period maintain hippocampal seizures via local aberrant excitatory circuitsSignal Transduction and Targeted TherapyJune 7, 2023Liying Chen, Yingwei Xu, Heming Cheng, Zhongxia Li, Nanxi Lai, Menghan Li, Yeping Ruan, Yang Zheng, Fan Fei, Cenglin Xu, et al.DCX (1:400, Abcam, ab18723), GABA(1:600, Sigma, A2052) as well as DNA-denaturated BrdU (1:400, Abcam ab1893) by incubating the sections with primary antibodies diluted in phosphate-buffered saline with 0.15%

(7) tRF-Gln-CTG-026 ameliorates liver injury by alleviating global protein synthesisSignal transduction and targeted therapyApril 3, 2023Ying S, Li P, Wang J, Chen K, Zou Y, Dai M, Xu K, Feng G, Zhang C, Jiang H, et al.polyclonal anti-Ki-67 (1 μg/mL, Thermo, PA5-19462), and sheep polyclonal antibody to BrdU (10 μg/mL, Abcam, ab1893). Alexa Fluor 488- and Alexa Fluor 594-conjugated secondary antibodies (Thermo) were used in a dilution

(8) Temporal origin of mouse claustrum and development of its cortical projectionsCerebral CortexMarch 21, 2023Anna Hoerder-Suabedissen, Gabriel Ocana-Santero, Thomas H Draper, Sophie A Scott, Jesse G Kimani, Andrew M Shelton, Simon J B Butt, Zoltán Molnár, Adam M Packer(1:500, Abcam, ab7349), rabbit anti-parvalbumin (1:500, Swant, P27a), and sheep anti-BrdU (1:500, Abcam, ab1893). The following secondary antibodies were used (all at 1:500): donkey anti-goat AlexaFluor594 (Life Technologies

(9) Huntingtin-associated protein 1 ameliorates neurological function rehabilitation by facilitating neurite elongation through TrKA-MAPK pathway in mice spinal cord injuryFrontiers in molecular neuroscienceJanuary 1, 2023Miao L, Qing SW, Tao L.μg/mL), GFAP (ab7260, Abcam, 1:1,000 dilution), NeuN (ab104225, Abcam, 1:1,000 dilution), and BrdU (ab1893, Abcam, 1:400 dilution) at 4°C overnight. The cells were washed three times with PBS, incubated with

(10) Histopathologic and transcriptomic phenotypes of a conditional RANKL transgenic mouse thymusCytokineDecember 1, 2022Szwarc MM, Hai L, Maurya VK, Rajapakshe K, Perera D, Ittmann MM, Mo Q, Lin Y, Bettini ML, Coarfa C, et al.(1:200); Cell Signaling Technology Inc., Danvers, MA); sheep polyclonal to 5-bromo-2-deoxyuridine (BrdU) (ab1893 (1:100); Abcam Inc.); a goat polyclonal anti-mouse RANKL (TRANCE; AF462 (1: 200) R&D Systems, Minneapolis,

(11) Nicotinamide reverses deficits in puberty-born neurons and cognitive function after maternal separationJournal of NeuroinflammationSeptember 21, 2022Keke Hao et al.anti-Iba1 (1:100, ab5076, Abcam), mouse anti-NeuN (1:100, ab104224, Abcam), goat anti-BrdU (1:100, ab1893, Abcam), or rabbit anti-c-fos (1:100, ab190289, Abcam) followed by incubation with an Alexa Fluor CY3-conjugated

(12) A Panax quinquefolius-Based Preparation Prevents the Impact of 5-FU on Activity/Exploration Behaviors and Not on Cognitive Functions Mitigating Gut Microbiota and Inflammation in MiceCancers (Basel)September 10, 2022Renaud Parment et al.overnight at 4 °C with the following primary antibodies: sheep anti-BrdU (1:100, Abcam, Paris, France, ab1893), rabbit anti-NeuN (1:1000, Abcam, ab104225), goat anti-glial fibrillary acidic protein (GFAP) (1:1000,

(13) The DEAD-box helicase Hlc regulates basal transcription and chromatin opening of stress-responsive genesNucleic Acids ResearchSeptember 9, 2022Ruirui Jia, Jiamei Lin, Jin You, Shi Li, Ge Shan, Chuan Huangthen purified using Dynabeads® Protein G (Thermo Fisher Scientific, 10003D) and anti-BrUTP (Abcam, ab1893). Efficient nascent RNA purification was verified by measuring the level of the MtnA precursor mRNA

(14) Intermittent hypoxia in a mouse model of apnea of prematurity leads to a retardation of cerebellar development and long-term functional deficitsCell & BioscienceSeptember 6, 2022S. Leroux, A. Rodriguez-Duboc, A. Arabo, M. Basille-Dugay, D. Vaudry, D. Burelantibody,Marker,Dilution,Species,Provider,Secondary antibody,Incubation time BrDU,Proliferating cells,1/400,Sheep,Abcam (#ab1893),DAS-633,O/N at 4 °C Calbindin,Purkinje cells,1/1000,Mouse,Sigma-Aldrich (#C9848),DAM-594,O/N (or 72

(15) The latent dedifferentiation capacity of newt limb muscles is unleashed by a combination of metamorphosis and body growthScientific reportsAugust 1, 2022Yu ZY, Shiga S, Casco-Robles MM, Takeshima K, Maruo F, Chiba C.min, and then BrdU-incorporated nuclei were labelled with sheep polyclonal anti-BrdU antibody (1:500; ab1893; Abcam) and donkey anti-sheep IgG (H + L) antibody conjugated with Alexa Fluor 350 (1:500; A-21097;

(16) A Short-Term Sucrose Diet Impacts Cell Proliferation of Neural Precursors in the Adult HypothalamusNutrientsJune 21, 2022Antonia Recabal, Sergio López, Magdiel Salgado, Alejandra Palma, Ana M. Obregón, Roberto Elizondo-Vega, Juan C. Sáez, María Á. García-Roblesanti-vimentin antibody (1:400, Millipore Cat# MAB3400), sheep anti-BrdU antibody (1:1000, Abcam, Cat# AB1893), goat anti-sheep Cy3, donkey anti-chicken Cy2, donkey anti-mouse Alexa 488. All secondary antibodies

(17) Integrated Multi-Omics Analysis Identified PTPRG and CHL1 as Key Regulators of Immunophenotypes in Clear Cell Renal Cell Carcinoma(ccRCC)Frontiers in OncologyMarch 30, 2022Xing Zeng, Le Li, Zhiquan Hu, Dan Pengincubating for another 4 h. Then, these cells were washed three times by PBS and 300 μl anti-BrdU (ab1893, Abcam, Cambridge, UK, dilution of 1:1,000) was supplemented to these cells overnight at room temperature.

(18) Krüppel-like factor 7 attenuates hippocampal neuronal injury after traumatic brain injuryNeural regeneration researchMarch 1, 2022Li WY, Fu XM, Wang ZD, Li ZG, Ma D, Sun P, Liu GB, Zhu XF, Wang Y.coronally sectioned at 25 μm. Sections were incubated with primary antibodies against BrdU (1:1000, Cat# ab1893, Abcam), doublecortin (DCX; Cat# 48-1200, 1:500, Invitrogen, Carlsbad, CA, USA), glial fibrillary acidic

(19) Hypophosphorylated pRb knock-in mice exhibit hallmarks of aging and vitamin C-preventable diabetesThe EMBO journalFebruary 15, 2022Jiang Z, Li H, Schroer SA, Voisin V, Ju Y, Pacal M, Erdmann N, Shi W, Chung PED, Deng T, et al.sucrose, frozen, cryo‐sectioned, and immunostained with the following antibodies: BrdU (1:500; Abcam Ab1893‐125), Ki67 (1:300; BD Pharmingen 550609), PH3 (1:500; Upstate 06‐570), GFP (1:1,000; Abcam Ab 6662)

(20) IL-1 reprogramming of adult neural stem cells limits neurocognitive recovery after viral encephalitis by maintaining a proinflammatory stateBrain, behavior, and immunityJanuary 1, 2022Soung AL, Davé VA, Garber C, Tycksen ED, Vollmer LL, Klein RS.analyses: NeuN (1:1000; Cell Signaling, cat no. 12943S, clone D3S3I), BrdU (1:250; Abcam, cat. no. ab1893, polyclonal), doublecortin (1:125; Cell Signaling, cat. no. 4604S, polyclonal), GFAP (1:250; Thermo,

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12/20/2022Reliable Proliferation Marker for NeurogenesisDr. Luiz Alexandre MagnoI have used this antibody to detect astrocyte proliferation induced by peripheral inflammation. It has worked very well in mouse sample without performing any special step for immunofluorescence.

Anti-BrdU antibody - Proliferation Marker from Abcam

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Anti-BrdU antibody - Proliferation Marker from Abcam

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