Fig 1: Expression levels of the selected pre-DM bio-markers in the liver samples of control and HFD-fed WT mice: (A–E) mRNA expression of Fcgr2b, Lgals1, Vcam1, Igfbp5, and Gas6 in the liver from control and HFD-fed WT mice (n = 5). (F) Protein lysates from the livers of control and HFD-fed WT mice were immunoblotted with indicated antibodies. β-actin was used as a loading control (n = 5). * p < 0.05, ** p < 0.01, and *** p < 0.001 control vs. HFD-fed WT mice at the respective weeks. Fcgr2b: Fc receptor, IgG, low affinity Iib, Lgals1: lectin, galactose binding, soluble 1, Vcam1: vascular cell adhesion molecule 1, Igfbp5: insulin-like growth factor binding protein 5, Gas6: growth arrest-specific 6.