Fig 1: Immunoblot analysis shows increased cardiac SCD and pro-apoptotic p53 protein levels of Tg-SCD mice. (a) Immunoblot detection (IB) of SCD-Scd1 was performed in cardiac lysates of 8-month-old Tg-SCD mice and compared to age-matched, non-transgenic B6 mice (left panel). The right panel shows quantitative data (n = 6 mice per group); (b) immunoblot detection of p53 (IB: p53) with p53-specific antibodies in lysates of Tg-SCD hearts compared to non-transgenic B6 mice (left panel), and quantitative immunoblot data (right panel; n = 6 mice per group). The lower immunoblot detects Gnb2 as a loading control. P values are indicated and were determined by the unpaired, two-tailed t-test.