BAF (A-11) Antibody sc-166324 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemBAF (A-11) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-166324
Antibody TypePrimary Antibodies
NCBI Gene AliasesBCRP1, Bcrp1, BAF, NGPS, L2bp1/Baf, Baf, D14S1460
CloneA-11
Quantity200 µg/ml
ApplicationsWB, IP, IF, ELISA
ClonalityMonoclonal
Reactivitymouse, rat, human
FormatPurified
Conjugate/TagUnconjugated
Hostmouse
NCBI Full Gene NameBAF nuclear assembly factor 1
Targetbarrier-to-autointegration factor
IsotypeIgG2a

Questions and Answers

Can this BAF antibody be used for studying its role in nuclear assembly during mitosis?

Yes, the BAF (A-11) antibody has been utilized in studies investigating its role in nuclear assembly. For instance, research has shown that BAF is crucial for guiding membranes to form a single nucleus during mitotic exit, as it facilitates DNA cross-bridging among chromosomes, which is essential for preventing nuclear fragmentation ().

Is this antibody effective for immunofluorescence applications?

The BAF (A-11) antibody has been successfully used in immunofluorescence applications, as indicated in multiple studies. For example, it was employed to visualize BAF accumulation at nuclear membrane rupture sites, demonstrating its utility in assessing protein localization in live-cell imaging contexts ().

What species can this antibody be used on for research purposes?

The BAF (A-11) antibody is reactive with multiple species, including human (Homo sapiens), mouse (Mus musculus), and rat (Rattus norvegicus), making it versatile for various experimental models.

Are there any known implications of BAF in tumor immunity that this antibody could help investigate?

Yes, the BAF antibody can be instrumental in studies exploring its role in tumor immunity. Research has indicated that targeting BANF1 (the protein encoded by the BAF gene) can enhance antitumor immune responses and improve the efficacy of immunotherapy, particularly through the cGAS-STING pathway ().

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) ER-tethering directs TREX1 penetration of a BAF-dependent barrier at micronucleiMol CellMarch 19, 2026Yanyang Chen, Roshan Xavier Norman, Eléonore Toufektchan, Xiaohan Luan, Abraham Shim, Hannah Rosenberg, Marton Tibor Kovacs, Ashley Nichols, James Hickling, Paolo Cifani, et al.solution (advansta) for 1 hour at room temperature and incubated with primary antibody (Santa Cruz, sc-166324) diluted 1:500 in blocking solution overnight at 4 °C. Membranes were washed four times in 1X TBS-T

(2) Non-random spatial organization of telomeres varies during the cell cycle and requires LAP2 and BAFiScienceApril 19, 2024Keller D, Stinus S, Umlauf D, Gourbeyre E, Biot E, Olivier N, Mahou P, Beaurepaire E, Andrey P, Crabbe L.sc-69879, RRID:AB_1119529" Actin-HRP,Santa Cruz,"Cat# sc-47778, RRID:AB_626632" BAF,Santa Cruz,"Cat# sc-166324, RRID:AB_2061087" Cep152,SIGMA,"Cat# HPA039408, RRID:AB_1079528" Chmp2A,Proteintech,"Cat# 10477-1-AP,

(3) A high-content screen reveals new regulators of nuclear membrane stabilityScientific reportsMarch 12, 2024Gunn AL, Yashchenko AI, Dubrulle J, Johnson J, Hatch EM.Thermo Fisher). For the BAF immunoblot: blocked membranes were incubated for 24 h in anti BAF (1:250, cat# sc-166324, Santa Cruz), washed, and incubated for 45 min at room temp. in goat anti-mouse HRP (1:5000, cat# G21040,

(4) Inhibition of tumor intrinsic BANF1 activates antitumor immune responses via cGAS-STING and enhances the efficacy of PD-1 blockadeJournal for immunotherapy of cancerAugust 1, 2023Wang M, Huang Y, Chen M, Wang W, Wu F, Zhong T, Chen X, Wang F, Li Y, Yu J, et al.online supplemental table 2. The following antibodies were used for western blot: BANF1 (Santa Cruz, #sc-166324), cGAS (Cell Signaling Technology, #31659), STING (Cell Signaling Technology, #13647), p-STING (Cell Signaling Technology

(5) Emerin prevents BAF-mediated aggregation of lamin A on chromosomes in telophase to allow nuclear membrane expansion and nuclear lamina formationMolecular Biology of the CellDecember 1, 2022L. Snyers, R. Löhnert, K. Weipoltshammer, C. Schöferthe 80-kDa LAP2α isoform (Figure 1). BAF was detected using mouse monoclonal anti-BAF antibody A-11 (sc-166324, Santa Cruz Biotechnology). Rabbit antibody against GAPDH was used for the loading control (G9545, Sigma,

(6) Dissecting the roles of Haspin and VRK1 in histone H3 phosphorylation during mitosisScientific reportsJuly 1, 2022Cartwright TN, Harris RJ, Meyer SK, Mon AM, Watson NA, Tan C, Marcelot A, Wang F, Zinn-Justin S, Traktman P, et al.

(7) Dysregulation of Cellular VRK1, BAF, and Innate Immune Signaling by the Vaccinia Virus B12 PseudokinaseJournal of virologyJune 8, 2022Linville AC, Rico AB, Teague H, Binsted LE, Smith GL, Albarnaz JD, Wiebe MS.,Atlas Antibodies,BANF1,"1:1,000",HPA039242 Mouse monoclonal anti-BAF,Santa Cruz Biotechnology,A-11,1:500,sc-166324 Rabbit polyclonal anti-BAF rabbit (total),Laboratory of Matthew Wiebe,NAa,"1:3,000",NA Rabbit polyclonal

(8) Endomembranes promote chromosome missegregation by ensheathing misaligned chromosomesThe Journal of cell biologyJune 6, 2022Ferrandiz N, Downie L, Starling GP, Royle SJ.

(9) Emerin Represses STAT3 Signaling through Nuclear Membrane-Based Spatial ControlInternational journal of molecular sciencesJune 22, 2021Lee B, Lee S, Lee Y, Park Y, Shim J.EDMD model, mouse embryonic fibroblasts (MEFs) 15378, 1:4500), Lamin A/C (#sc-20681, 1:4500), BAF (#sc-166324, 1:5000), Bcl2 (#sc-7382, from Lmna−/− mice, has a fast-growing phenotype similar to emerin null fibroblasts

(10) BAF restricts cGAS on nuclear DNA to prevent innate immune activationScience (New York, N.Y.)August 14, 2020Guey B, Wischnewski M, Decout A, Makasheva K, Kaynak M, Sakar MS, Fierz B, Ablasser A.

(11) BAF facilitates interphase nuclear membrane repair through recruitment of nuclear transmembrane proteinsMolecular biology of the cellJuly 15, 2020Young AM, Gunn AL, Hatch EM.normalizing to loading controls. Primary antibodies (human proteins) Mouse anti–BAF (1:250; clone A11; sc-166324; Santa Cruz Biotechnology) Rabbit anti–Lamin A/C (1:10,000; clone EPR4100; ab108595; Abcam) Mouse anti–Lamin

(12) Barrier-to-Autointegration Factor 1 Protects against a Basal cGAS-STING ResponsemBioMarch 10, 2020Ma H, Qian W, Bambouskova M, Collins PL, Porter SI, Byrum AK, Zhang R, Artyomov M, Oltz EM, Mosammaparast N, et al.Membranes were blotted with the following primary antibodies: Banf1 (BAF antibody A-11 [Santa Cruz, sc-166324]; 1:500), Irf3 (CST 4302; 1:1,000), cGAS (CST 31659; 1:1,000), Irf1 (CST 8478; 1:1,000), Aim2 (CST 63660;

(13) DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic ChromosomesCellAugust 24, 2017Samwer M, Schneider MWG, Hoefler R, Schmalhorst PS, Jude JG, Zuber J, Gerlich DW.1:1000),Abcam,ab16048Lab ID#217 BAF (A-11) (Immunoblot (IB): 1:250; IF: 1:200),Santa Cruz Biotechnology,sc-166324 Lab ID #224 Nuclear Pore Complex Proteins (mAb414) (IF: 1:1000),Eurogentec,MMS-120P-0500 Emerin (IF

(14) Emerin suppresses Notch signaling by restricting the Notch intracellular domain to the nuclear membraneBiochimica et biophysica acta. Molecular cell researchFebruary 1, 2017Lee B, Lee TH, Shim J.

(15) The lamin-A/C-LAP2α-BAF1 protein complex regulates mitotic spindle assembly and positioningJournal of cell scienceAugust 1, 2015Qi R, Xu N, Wang G, Ren H, Li S, Lei J, Lin Q, Wang L, Gu X, Zhang H, et al.distinct functions in interphase and mitosis. MATERIALS AND METHODS Antibodies Two antibodies against BAF1 (sc-166324 and sc-33787) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA), and two monoclonal antibodies

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BAF (A-11) Antibody

BAF (A-11) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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BAF (A-11) Antibody from Santa Cruz Biotechnology, Inc.

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