Fig 1: AEAB suppresses cyclin B, CDC2, and CHK2 gene expression in Hone-1 and Detroit 562 cells. Cells were treated with AEAB for 24 h. (A) The RT-PCR of cyclin B, CDC2, and CHK2 gene expression following exposure to the AEAB. (B) The panels indicate quantitative RT-PCR (qRT-PCR) analysis of cyclin B, CDC2, and CHK2 gene expression standardized against the level of GAPDH in cells. All data are reported as a percentage of 2-ΔΔCt at least three separate experiments (mean ± SEM). Statistical analysis was performed using the t-test, with differences considered significant at a level of *P < 0.05 versus the 0 mg/mL AEAB control group.