Fig 1: The percentages of CD206+ cells (M2 macrophages) in peripheral blood mononuclear cells (PBMNCs) compared between PBMNCs cultured in Quality and Quantity culture media (QQ-media) and PBMNCs cultured in standard culture media. All experiments were performed in triplicate. Forward and side scatter plots were used to gate the entire population of mononuclear cells. The gated cells were then evaluated to identify CD3 and CD11c negative cells. The CD3 and CD11c negative cells were then gated to assess the population of CD206+ cells. Student's t-test was used for phenotypic analysis