Anti-Histone H4 antibody - ChIP Grade ab10158 from Abcam

Product Specs
ItemAnti-Histone H4 antibody - ChIP Grade
CompanyAbcam
Price Pricing Info Supplier Page View Supplier Product Page
Catalog Numberab10158
NCBI Gene AliasesH4/p
Antibody TypePrimary
Quantity100 UG
ApplicationsChIP, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot
HostRabbit
IsotypeIgG
ClonalityPolyclonal
Conjugate/TagUnconjugated
Concentration1 mg/ml
Research AreaEpigenetics
Gene NameHIST4H4
ReactivityHuman, Mouse, Rat
TargetHistone H4

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Effects of the G-quadruplex-binding drugs quarfloxin and CX-5461 on the malaria parasite Plasmodium falciparumInternational Journal for Parasitology: Drugs and Drug ResistanceNovember 24, 2023Holly M. Craven, Guilherme Nettesheim, Pietro Cicuta, Andrew M. Blagborough, Catherine J. Merrickovernight with either 1:1000 anti-phospho-histone-H2A.X Ser 139 (#9718, CellSignalling) or anti H4 (ab10158, Abcam), rinsed, then probed with 1:2000 goat anti rabbit IgG-HRP (Abcam). Blots were developed using

(2) Altered chromatin occupancy of patient-associated H4 mutants misregulate neuronal differentiationbioRxivOctober 6, 2023Lijuan Feng, Douglas Barrows, Liangwen Zhong, Kärt Mätlik, Elizabeth G. Porter, Annaelle M. Djomo, Iris Yau, Alexey A. Soshnev, Thomas S. Carroll, Duancheng Wen, et al.HA (Biolegend 901501, 1:1000), H3 (Abcam 1791, 1:10,000), H3.3 (Millipore 09–838, 1:1000), H4 (Abcam ab10158, 1:5000). Mononucleosome immunoprecipitation (Mono-IP) 3HA-tagged H4WT, H4K91R, or H4K91Q were cloned

(3) Lactate as a major epigenetic carbon source for histone acetylation via nuclear LDH metabolismExperimental & Molecular MedicineOctober 2, 2023Yong Jin An, Sihyang Jo, Jin-Mo Kim, Han Sun Kim, Hyun Young Kim, Sang-Min Jeon, Dawool Han, Jong In Yook, Keon Wook Kang, Sunghyouk Parkanti-Cytochrome C (sc-13560), anti-β-actin (sc-47778, Santa Cruz, Dallas, TX, USA), anti-Histone H4 (ab10158, Abcam, Cambridge, UK), anti-acetyl-histone H4 (#06-866, Merck, Kenilworth, NJ, USA), anti-mouse (sc-2005),

(4) Cla4 phosphorylates histone methyltransferase Set1 to prevent its degradation by the APC/C Cdh1 complexScience AdvancesSeptember 29, 2023Xuanyunjing Gong, Shanshan Wang, Qi Yu, Min Wang, Feng Ge, Shanshan Li, Xilan Yuconstitutive promoter. Antibodies Antibodies against histone H3 (1: 5000; ab1791) and histone H4 (1:5000; ab10158) were purchased from Abcam; antibodies against Set1 (1:1000; sc-101858) and Rtf1 (1:500; sc-26327) were

(5) Neutrophil extracellular traps and extracellular histones potentiate IL-17 inflammation in periodontitisJournal of Experimental MedicineSeptember 4, 2023Tae Sung Kim, Lakmali M. Silva, Vasileios Ionas Theofilou, Teresa Greenwell-Wild, Lu Li, Drake Winslow Williams, Tomoko Ikeuchi, Laurie Brenchley, Thomas H. NIDCD/NIDCR Genomics and Computational Biology Core, Patricia I. Bugge, et al.anti–histone H3 (citrulline R2+R8+R17; ab5103; Abcam), anti–histone H3 (ab1791; Abcam), anti–histone H4 (ab10158; Abcam), and anti–β-actin (4970; Cell Signaling) diluted 1:1,000. The membranes were incubated with

(6) Chromatin remodeling of histone H3 variants by DDM1 underlies epigenetic inheritance of DNA methylationCellSeptember 1, 2023Seung Cho Lee, Dexter W. Adams, Jonathan J. Ipsaro, Jonathan Cahn, Jason Lynn, Hyun-Soo Kim, Benjamin Berube, Viktoria Major, Joseph P. Calarco, Chantal LeBlanc, et al.anti-H3,Abcam,Cat#Ab1791 Rabbit polyclonal anti-H4K16ac,EMD millipore,Cat#07-329 Rabbit polyclonal anti-H4,Abcam,Cat#ab10158 GFP-trap magnetic agarose beads,ChromoTek,Cat#gtma-10 RFP-trap magnetic agarose beads,ChromoTek,Cat#rtma-10 Rabbit

(7) Heterogeneous non-canonical nucleosomes predominate in yeast cells in situeLifeJuly 28, 2023Zhi Yang Tan, Shujun Cai, Alex J Noble, Jon K Chen, Jian Shi, Lu Ganpolyclonal),Abcam,"Cat# ab1791, RRID:AB_302613",WB (1:1000) Antibody,Anti-H4 (Rabbit polyclonal),Abcam,"Cat# ab10158, RRID:AB_296888",WB (1:1000) Antibody,Anti-GFP (Mouse monoclonal),Santa Cruz Biotechnology,"Cat# sc-9996,

(8) Gain and loss of function variants in EZH1 disrupt neurogenesis and cause dominant and recessive neurodevelopmental disordersNature communicationsJuly 11, 2023Gracia-Diaz C, Zhou Y, Yang Q, Maroofian R, Espana-Bonilla P, Lee CH, Zhang S, Padilla N, Fueyo R, Waxman EA, et al.anti-H3K27me3 (EMD Millipore, 07-449), 1:5,000; anti-β-actin (GenScript, A00702), 1:10,000 anti-H4 (Abcam, ab10158)). The next day the membrane was washed three times with TBST, incubated for 1 h with the corresponding

(9) SETD4 Confers Cancer Stem Cell Chemoresistance in Nonsmall Cell Lung Cancer Patients via the Epigenetic Regulation of Cellular QuiescenceStem Cells InternationalMay 27, 2023Yuehong Wang, Yuman Yu, Weijun Yang, Linying Wu, Yaoshun Yang, Qianyun Lu, Jianying Zhou(Cell Signaling Technology; 2616S), GAPDH (Cell Signaling Technology; 2118S), H3 (Abcam; ab1791), H4 (Abcam; ab10158), CD133 (HuaAn-Biotec; EM1701-28 for IF assays), CD133 (CD133 MicroBead Kit; Miltenyi Biotec; 130-100-857),

(10) Histone H3 serotonylation dynamics in dorsal raphe nucleus contribute to stress-induced gene expression and behaviorbioRxivMay 9, 2023Amni Al-Kachak, Sasha L. Fulton, Lorna A. Farrelly, Ashley E. Lepack, Ryan M. Bastle, Lingchun Kong, Flurin Cathomas, Emily L. Newman, Caroline Menard, Aarthi Ramakrishnan, et al.anti-H3Q5ser (1:500, MilliporeSigma; ABE1791,), rabbit anti-H3 (1:50000, Abcam ab1791), H4 (1:10000, Abcam; ab10158), H3.3 (1:2000, MilliporeSigma; 09-838,), FLAG (1:5000, Sigma; F3165,) and rabbit anti-Gapdh (1:10000,

(11) Cardiomyocyte-targeting exosomes from sulforaphane-treated fibroblasts affords cardioprotection in infarcted ratsJournal of Translational MedicineMay 9, 2023Gaia Papini, Giulia Furini, Marco Matteucci, Vanessa Biemmi, Valentina Casieri, Nicole Di Lascio, Giuseppina Milano, Lucia Rosa Chincoli, Francesco Faita, Lucio Barile, et al.S.r.l., Milan, Italy, 06–866, lot number: 2664262, 1:1000), total H4 histone (Abcam, Cambridge, UK, ab10158, lot number: GR194493-1 and GR114730-1, 1:500), acetylated H3 histone (Acetyl K14) (Abcam, Cambridge,

(12) Ageing-associated changes in transcriptional elongation influence longevityNatureApril 27, 2023Cédric Debès, Antonios Papadakis, Sebastian Grönke, Özlem Karalay, Luke S. Tain, Athanasia Mizi, Shuhei Nakamura, Oliver Hahn, Carina Weigelt, Natasa Josipovic, et al.and H4 overexpression was verified by western blot with anti-H3 and anti-H4 antibodies (ab1791 and ab10158, Abcam, respectively). All further assays were repeated in proliferating cells and cells at the senescence

(13) The RNA-binding protein landscapes differ between mammalian organs and cultured cellsNature communicationsApril 12, 2023Perez-Perri JI, Ferring-Appel D, Huppertz I, Schwarzl T, Sahadevan S, Stein F, Rettel M, Galy B, Hentze MW.RRID:AB_881762, 1:1000), Beta-actin (Actb) (Sigma-Aldrich, A1978, RRID:AB_476692, 1:5000), and Histone H4 (Abcam, ab10158, RRID:AB_296888, 1:4000). As secondary antibodies anti-rabbit (Abcam, ab97051, RRID:AB_10679369, 1:5000)

(14) HP1 proteins regulate nucleolar structure and function by secluding pericentromeric constitutive heterochromatinNucleic acids researchJanuary 11, 2023Ballmer D, Tardat M, Ortiz R, Graff-Meyer A, Ozonov EA, Genoud C, Peters AH, Fanourgakis G.anti-H3 (Abcam ab1791, 1:2000), polyclonal anti-H4K20me3 (IMP 0083, 1:2000), polyclonal anti-H4 (Abcam ab10158, 1:4000), polyclonal anti-Myc (Abcam ab9132, 1:1000), monoclonal anti-GFP (Roche 11814460001, 1:1000).

(15) FgSnt1 of the Set3 HDAC complex plays a key role in mediating the regulation of histone acetylation by the cAMP-PKA pathway in Fusarium graminearumPLOS GeneticsDecember 7, 2022Chen Gong, Daiying Xu, Daiyuan Sun, Jiangang Kang, Wei Wang, Jin-Rong Xu, Xue Zhang(ab194352) antibodies from Abcam (Cambridge, UK). Detection with the anti-H3 (ab209023, Abcam), and anti-H4 (ab10158, Abcam) antibodies were used as the loading controls. The band intensity was quantified using ImageJ

(16) SESAME-catalyzed H3T11 phosphorylation inhibits Dot1-catalyzed H3K79me3 to regulate autophagy and telomere silencingNature communicationsDecember 6, 2022He F, Yu Q, Wang M, Wang R, Gong X, Ge F, Yu X, Li S.quantified with ImageJ. Antibodies Antibodies against anti-H3 (1:5000; ab1791), anti-H4 (1:5000; ab10158) and H3pT11 (1:5000; ab5168) were purchased from Abcam; antibodies against H3K79me1 (1: 1000; A2367),

(17) Acetylation State of Lysine 14 of Histone H3.3 Affects Mutant Huntingtin Induced PathogenesisInternational Journal of Molecular SciencesDecember 2, 2022Anikó Faragó, Nóra Zsindely, Anita Farkas, Alexandra Neller, Fruzsina Siági, Márton Richárd Szabó, Tamás Csont, László BodaiAgilent/Dako); rabbit polyclonal anti-H3 (1:5000, ab1791, Abcam); and rabbit polyclonal anti-H4 (1:1000, ab10158, Abcam) with goat–anti-rabbit IgG-HRP (1:5000, P0448, Agilent/Dako). 4.6. Validation of Transgene Expression The

(18) Histone H3 clipping is a novel signature of human neutrophil extracellular trapseLifeOctober 25, 2022Dorothea Ogmore Tilley, Ulrike Abuabed, Ursula Zimny Arndt, Monika Schmid, Stefan Florian, Peter R Jungblut, Volker Brinkmann, Alf Herzig, Arturo ZychlinskyMotif:61277,Western blot (1:15000) Antibody,Rabbit polyclonal Histone H4 C-terminal; αH4-C,Abcam,Abcam:ab10158,Western blot (1:5000) Antibody,Rabbit monoclonal anti-histone H4 N terminal,"Upstate Millipore, Sigma

(19) The histone deacetylases Rpd3 and Hst1 antagonistically regulate de novo NAD+ metabolism in the budding yeast Saccharomyces cerevisiaeJournal of Biological ChemistryOctober 1, 2022Benjamin Groth, Chi-Chun Huang, Su-Ju Linalong with the H4 protein itself, was precipitated as aforementioned using the following antibodies: ab10158, Abcam (H4); 71-290-6, Invitrogen (H4K5-Ac); ab15823, Abcam (H4K8-Ac); ab46983, and Abcam (H4K12-Ac).

(20) Phosphorylation of Jhd2 by the Ras-cAMP-PKA(Tpk2) pathway regulates histone modifications and autophagyNature communicationsSeptember 27, 2022Yu Q, Gong X, Tong Y, Wang M, Duan K, Zhang X, Ge F, Yu X, Li S.software (v.1.8.0). Antibodies Antibodies against anti-H3 (1: 5000; ab1791), histone H4 (1:5000; ab10158) and H3K14ac (1:2000; ab52946) were purchased from Abcam; antibodies against H3K4me3 (1:3000; A2357),

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6/7/2016Anti-Histone H4 Antibody Abcam Review Tamanna HossinWe study epigenetics in mouse ES cells. When looking at histone modifications, we use H4 as the loading control.

Anti-Histone H4 antibody - ChIP Grade from Abcam

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Anti-Histone H4 antibody - ChIP Grade from Abcam

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