Anti-Histone H4 Antibody Abcam Review

June 07, 2016

Genetics
Yale
Tech

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Abcam

Product Name:

Anti-Histone H4 antibody - ChIP Grade

Catalog Number:

ab10158

We study epigenetics in mouse ES cells. When looking at histone modifications, we use H4 as the loading control.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse ES cells histones

Primary Incubation

1:1000 4C overnight

Blocking Agent

5% milk

Secondary Incubation

1:3000 in 5% milk for one hour at RT

Tertiary Incubation

N/A

Detection

Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad

Results Summary

Pretty good for showing relative loading amounts between lanes. There is a second band though, which is unexpected.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Good loading control

The Bad

Unexpected band

The Bottom Line

Good for loading control.

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