Fig 1: SDOS and TRAP1 are protein partners. (A) Volcano plot showing SDOS (left) and TRAP1 (right) protein partners identified by MS (dark-red = significant, light blue = intermediate, light gray = not significant). Relevant protein-partners (RPS28, SDOS, TRAP1, TP53BP1) are highlighted. (B) Silver staining showing the pattern of SDOS-eGFP and TRAP1-eGFP IPs (specific band indicated with asterisk). (C) Co-immunoprecipitation of TRAP1 and SDOS from HeLa cells following induction of TRAP1-eGFP and eGFP for 24 h. Total lysates were immunoprecipitated with GFP-Trap_MA magnetic agarose beads and analyzed by WB with the indicated antibodies. (D) Representative image of PLA showing the interaction of SDOS with TRAP1 in HCT116 cells. Positive signals of interaction are shown as red dots, nuclei are stained with DAPI (blue). Negative control has been obtained by hybridizing cells with TRAP1 antibody only. (E) Representative image of PLA showing the interaction of SDOS with rpS28 in HeLa cells, following induction of SDOS-eGFP or unfused eGFP (negative control) for 24 h and hybridization with anti-GFP and anti-rpS28 antibodies. Positive signals of interaction are shown as red dots, nuclei are stained with DAPI (blue). (F) Computational prediction of SDOS protein partners network using STRING database (https://string-db.org). Interactions include direct (physical) and indirect (functional) associations. Light-blue edge: known interaction from curated databases; purple edge: experimentally determined interaction; green edge: textmining; black edge: co-expression; dark-blue edge: protein homology.