AMV Reverse Transcriptase from New England Biolabs

Product Specs
ItemAMV Reverse Transcriptase
CompanyNew England Biolabs
Price $320.00 Inquire
Catalog NumberM0277L
Quantity1000 units
Concentration10,000 units/ml
ApplicationsDNA Analysis
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to incorporate 1 nmol of dTMP into an acid-insoluble form in 10 minutes at 37°C using poly(rA)-oligo(dT) as template primer.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Infection of 5xFAD mice with a mouse-adapted SARS-CoV-2 does not alter Alzheimer's disease neuropathology yet induces widespread changes in gene expression across diverse cell typesAlzheimers DementApril 24, 2026Susana Furman, Latifa Zayou, Kate Inman Tsourmas, Dominic Ibarra Javonillo, Gema M. Olivarria, Yuting Cheng, Collin Pachow, Kellie Fernandez, Lucas Le, Robert A. Edwards, et al.“First Strand cDNA Synthesis” protocol by New England Biolabs, using AMV Reverse Transcriptase (New England Biolabs, M0277L), Random Hexamers (Invitrogen, N8080127), RNAse inhibitor (New England BiolabsM0314L), and AMV Buffer

(2) The human branchpoint-interacting stem-loop sequence and structure regulates U2 snRNA expression, branchpoint recognition, and the transcriptomeNucleic Acids ResMarch 26, 2026Meredith B Stevers, Sol Katzman, Melissa S Juricamagnesium chloride, 0.1 mM dATP, dTTP, dCTP, and ddGTP and 2 units of AMV reverse transcriptase (NEB, cat. M0277S). The reactions were incubated at 42°C for 70 min followed by addition of 10 volumes of 0.3 M sodium

(3) TOR-dependent regulation of the yeast homolog of the juvenile Batten Disease-associated gene CLN3Microb CellMarch 11, 2026Vijaykumar Pillalamarri, Samuel W.M. Gatesy, Amanda E. Grassel, Lucienna Wolf, Justin P. Whalley, David M. MuellerDNase (New England Biolabs, M0303S) treated total RNA. AMV reverse transcriptase (New England Biolabs, M0277L) and RNase H (New England Biolabs M0297) were used to make the cDNA. 5’ RACE-NGS data analysis The

(4) Cleavage of mRNAs by a minority of pachytene piRNAs improves sperm fitnessNatureFebruary 4, 2026Katharine Cecchini, Mina Zamani, Nandagopal Ajaykumar, Joel Vega-Badillo, Ayca Bagci, Shannon Bailey, Phillip D. Zamore, Ildar Gainetdinovethanol, cDNA synthesis was performed in 20 µl at 42 °C for 1 h using AMV reverse transcriptase (NEB, M0277), and 5 µl of the RT reaction was amplified in 25 µl using AccuPrime Pfx DNA polymerase (ThermoFisher,

(5) Infection of 5xFAD mice with a mouse-adapted SARS-CoV-2 does not alter Alzheimer’s disease neuropathology yet induces wide-spread changes in gene expression across diverse cell typesbioRxivDecember 24, 2025Susana Furman, Latifa Zayou, Kate Inman Tsourmas, Dominic Ibarra Javonillo, Gema M. Olivarria, Yuting Cheng, Collin Pachow, Kellie Fernandez, Lucas Le, Robert A. Edwards, et al.“First Strand cDNA Synthesis” protocol by New England Biolabs, using AMV Reverse Transcriptase (New England Biolabs, M0277L), Random Hexamers (Invitrogen, N8080127), RNAse inhibitor (New England BiolabsM0314L) and AMV Buffer

(6) Generation of a scFv Derived from an IgM-Producing Hybridoma for the Detection of REST Expression in Premalignant Lesions and Invasive Squamous Cell CarcinomaInt J Mol SciDecember 11, 2025Cynthia Rodríguez-Nava, Karen Cortés-Sarabia, Lidia Riaño-Umbarila, Baltazar Becerril-Luján, Yolanda Medina-Flores, Olga Mata-Ruíz, Lourdes Lloret-Sánchez, Berenice Illades-Aguiar, Luz Del Carmen Alarcón-Romero, Carlos Ortuño-Pinedacomplementary DNA (cDNA). We utilized the AMV RT-PCR kit (New England Biolabs, Ipswich, MA, USA, Cat# M0277S) by mixing the following components: 4 µL of DNase-free distilled water, 1 µL of dNTP, 2 µL of dT oligos,

(7) Extracellular vesicle-mediated gene editing for the treatment of non-syndromic progressive hearing loss in adult miceScience Translational MedicineNovember 12, 2025Xiaoshu Pan, Peixin Huang, Samantha S. Ali, Bryan Renslo, Zachary Greenberg, Nina Erwin, Yanjun Li, Zuo Ding, Tarun E. Hutchinson, Athanasia Warnecke, et al.was obtained from CADworks3D. Q5 high-fidelity DNA polymerase (M0491) and AMV reverse transcriptase (M0277) was purchased from New England Biolabs. Human bone marrow-derived conditioned culture medium and human

(8) The human branchpoint-interacting stem loop sequence and structure regulates U2 snRNA expression, branchpoint recognition, and transcriptomebioRxivSeptember 18, 2025Meredith B. Stevers, Sol Katzman, Melissa S. Juricamagnesium chloride, 0.1 mM dATP, dTTP, dCTP, and ddGTP and 2 units of AMV reverse transcriptase (NEB, cat M0277S). The reactions were incubated at 42°C for 70 mins followed by addition of 10 volumes of 0.3 M sodium

(9) Absolute quantification of mammalian microRNAs for therapeutic RNA cleavage and detargetingRNAAugust 1, 2025Carolyn Kraus, Jiayi Wang, Haiying Zheng, Jennifer Broderick, Nandagopal Ajaykumar, Mina Zamani, Mengqi Yang, Katharine Cecchini, Shun-qing Liang, Olena Kolumba, et al.ethanol, cDNA synthesis was performed in 20 µL at 42°C for 1 h using AMV reverse transcriptase (NEB, M0277), and 5 µL of the RT reaction was amplified in 25 µL using AccuPrime Pfx DNA polymerase (Fisher, 12344024;

(10) Prion-induced ferroptosis is facilitated by RAC3Nat CommunJune 25, 2025Hao Peng, Susanne Pfeiffer, Borys Varynskyi, Marina Qiu, Chanikarn Srinark, Xiang Jin, Xin Zhang, Katie Williams, Bradley R. Groveman, Simote T. Foliaki, et al.Subsequently, 2 μg of total RNA was reverse transcribed using the AMV Reverse Transcriptase Kit (NEB, M0277S). Quantitative PCR reactions were carried out on a LightCycler480 (Roche) instrument using Power SYBR

(11) Deep sequencing of yeast and mouse tRNAs and tRNA fragments using OTTReLifeApril 25, 2025Hans Tobias Gustafsson, Lucas Ferguson, Carolina Galan, Tianxiong Yu, Heather Upton, Ebru Kaymak, Zhiping Weng, Kathleen Collins, Oliver J Randowas then ethanol precipitated, and cDNA synthesis was performed with AMV reverse transcriptase (NEB, M0277L). cDNA was finally PCR amplified with standard Illumina sequencing primers with AccuPrime Pfx DNA polymerase

(12) Rapid and portable quantification of HIV RNA via a smartphone-enabled digital CRISPR device and deep learningSensors and Actuators ReportsApril 16, 2025Hoan T. Ngo, Patarajarin Akarapipad, Pei-Wei Lee, Joon Soo Park, Fan-En Chen, Alexander Y. Trick, Tza-Huei Wang, Kuangwen HsiehWarmStart® RTx reverse transcriptase (M0380; 15,000 U/ml), Avian Myeloblastosis Virus (AMV) reverse transcriptase (M0277; 10,000 U/mL), and bovine serum albumin (BSA; B9000; 20 mg/mL) were purchased from New England BioLabs

(13) Developing, Characterizing, and Modeling CRISPR-Based Point-of-Use Pathogen DiagnosticsACS Synthetic BiologyJanuary 17, 2025Jaeyoung K. Jung, Kathleen S. Dreyer, Kate E. Dray, Joseph J. Muldoon, Jithin George, Sasha Shirman, Maria D. Cabezas, Anne E. d’Aquino, Matthew S. Verosloff, Kosuke Seki, et al.primer binding. The first cDNA synthesis was initiated by adding 1 U/μL RT at final concentration (NEB #M0277L for AMV RT, NEB #M0368L for ProtoScript II RT, and NEB #M0253L for M-MuLV RT). After 20 min of incubation

(14) A non-canonical role for a small nucleolar RNA in ribosome biogenesis and senescenceCellAugust 22, 2024Yujing Cheng, Siwen Wang, He Zhang, Jong-Sun Lee, Chunyang Ni, Jason Guo, Eric Chen, Shenming Wang, Asha Acharya, Tsung-Cheng Chang, et al.Superscript IV Reverse Transcriptase,Invitrogen,Cat#18090010 AMV Reverse Transcriptase,New England Biolabs,Cat#M0277S Protein Synthesis Assay Kit,Cayman Chemical,Cat#601100 TURBO DNA-free kit,Invitrogen,Cat#AM1907 DIG RNA labeling mix

(15) Subnormothermic ex vivo lung perfusion possibly protects against ischemia-reperfusion injury via the mTORC-HIF-1α pathwayJournal of Thoracic DiseaseApril 30, 2024Jee Won Suh, Soo Jin Park, Young Wha Koh, Daun Seo, Seokjin Haammanufacturer’s protocol. For qRT-PCR, 1 µg RNA was reverse transcribed using AMV Reverse Transcriptase (#M0277L, New England Biolabs, Ipswich, MA, USA). Transcript levels of target genes were quantified with 2× KAPA

(16) Intracellular iron accumulation facilitates mycobacterial infection in old mouse macrophagesGeroScienceDecember 30, 2023Stephen K. Kotey, Xuejuan Tan, Owen Fleming, Ramakrishnama Raju Kasiraju, Audrey L. Dagnell, Kyle N. Van Pelt, Janet Rogers, Steven D. Hartson, Nidheesh Thadathil, Ramasamy Selvarani, et al.Scientific, Cat. no. ND-1000). cDNA was synthesized using NEB AMV Reverse Transcriptase (NEB, Cat. no. M0277) according to the manufacturer’s protocol. The cDNA samples were immediately stored at − 20 °C until

(17) Base-resolution quantitative DAMM-seq for mapping RNA methylations in tRNA and mitochondrial polycistronic RNAMethods in EnzymologyNovember 4, 2023Li-Sheng Zhang, Cheng-Wei Ju, Bochen Jiang, Chuan HeLS05006). 10× AMV Reverse Transcriptase Reaction Buffer (Included in New England BioLabs, cat. no. M0277S). Deoxynucleotide (dNTP) Solution Mix (10 mM) RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen,

(18) Understanding cancer drug resistance with Sleeping Beauty functional genomic screens: Application to MAPK inhibition in cutaneous melanomaiScienceOctober 20, 2023Eliot Y. Zhu, Jacob L. Schillo, Sarina D. Murray, Jesse D. Riordan, Adam J. DupuyTGGGATTG-3′, 5′-CTCATGATTACCGCAGCAAA-3′). cDNA was synthesized using NEB AMV reverse transcriptase (M0277S). RT-qPCR was performed using Taq polymerase with EvaGreen dye with total reaction volume of 12 uL.

(19) A maternally programmed intergenerational mechanism enables male offspring to make piRNAs from Y-linked precursor RNAs in DrosophilaNature Cell BiologyOctober 1, 2023Zsolt G. Venkei, Ildar Gainetdinov, Ayca Bagci, Margaret R. Starostik, Charlotte P. Choi, Jaclyn M. Fingerhut, Peiwei Chen, Chiraag Balsara, Troy W. Whitfield, George W. Bell, et al.ethanol, and cDNA synthesis was performed in 20 µl at 42 °C for 1 h using AMV reverse transcriptase (NEB, M0277) and 5 µl RT reaction was amplified in 25 µl using AccuPrime Pfx DNA polymerase (Thermo Fisher, 12344024;

(20) The fission yeast methyl phosphate capping enzyme Bmc1 guides 2′-O-methylation of the U6 snRNANucleic Acids ResearchSeptember 8, 2023Jennifer Porat, Viktor A Slat, Stephen D Rader, Mark A Bayfieldwith 1.5 mM (high concentration) or 0.1 mM (limiting concentration) dNTP mix and 2.5 U AMV-RT (NEB, M0277S) and 1 h incubation at 42°C. cDNA products were separated on 8% TBE–urea sequencing gels, dried, and

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