Amino Acid Mixture, Complete from Promega

Product Specs
ItemAmino Acid Mixture, Complete
CompanyPromega
Price $44.00Supplier Page View Company Product Page
Catalog NumberL4461
Quantity175microl
Molecular Weight~74kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) RNA G-quadruplexes promote codon repeat-associated ribosomal frameshifting in human genesNucleic Acids ResearchJanuary 16, 2026Xiuwen Li, Zhanbiao Li, Yingshui Zhou, Shimin Gong, Zhenjing Chen, Jingyang Li, Miaomiao Guo, Xiaoqian Gu, Fei Li, Jiayu Wei, et al.50% glycerol], 0.5 μl of 1 M KOAc, 0.2 μl of 50 mM Mg(OAc)2, 0.1 μl of 1 mM amino acid mix (Promega, L4461), 0.25 μl of RiboLock RNase Inhibitor (Thermo Fisher, EO0384), 10 ng of control mRNA (encoding Renilla

(2) Reprogramming neuroblastoma by diet-enhanced polyamine depletionNatureSeptember 24, 2025Sarah Cherkaoui, Christina S. Turn, Yuan Yuan, Wenyun Lu, Lifeng Yang, Matthew J. McBride, Caroline Eigenmann, George E. Allen, Olesya O. Panasenko, Lu Zhang, et al.acetate, 2 mM magnesium actetate, 1.75 mM ATP, 0.4 mM GTP, 50 μM complete amino acid mix (Promega, L4461), 20 mM creatine phosphate, 0.3 mg ml−1 creatine kinase, 500 ng RNA) for 3 h at 35 °C. Spermidine was

(3) Systematic analysis of nonsense variants uncovers peptide release rate as a novel modifier of nonsense-mediated mRNA decayCell GenomicsJuly 9, 2025Divya Kolakada, Rui Fu, Nikita Biziaev, Alexey Shuvalov, Mlana Lore, Amy E. Campbell, Michael A. Cortázar, Marcin P. Sajek, Jay R. Hesselberth, Neelanjan Mukherjee, et al.AppliChem,121515.1211 KOAc,Helicon,Am-0698-0.5 Mg(OAc)2,MERCK,105819 ATP,ThermoFisher Scientific,R0441 GTP,ThermoFisher Scientific,R0461 Amino Acids,Promega,L4461 Spermidine,Sigma-Aldrich,85558-5G Aminoacylated total rabbit tRNA,gift from Vladimir I. Katunin,N/A Creatine

(4) G-quadruplex topologies determine the functional outcome of guanine-rich bioactive oligonucleotidesNucleic Acids ResJune 28, 2025Prakash Kharel, Nupur Bhatter, Safiyah Zubair, Shawn M Lyons, Paul J Anderson, Pavel Ivanovphosphate, 0.01 μg/ml creatine kinase, 10 μM spermidine, and 10 μM l-amino acid mix, Promega®, Cat# L4461), 1 unit of RNase inhibitor (Promega®, Cat# N251B), and micrococcal nuclease (Thermo Fisher Cat# 88216)

(5) MCTS2 and distinct eIF2D roles in uORF-dependent translation regulation revealed by in vitro re-initiation assaysThe EMBO journalFebruary 1, 2025Meurs R, De Matos M, Bothe A, Guex N, Weber T, Teleman AA, Ban N, Gatfield D.assay About 6.5 µl of translation-competent extracts were freshly supplemented with 0.4 mM amino acids (L4461, Promega), 15 mM HEPES pH 7.3, 6 mM Creatine Phosphate, 102 ng/ml Creatine kinase, 28 mM potassium acetate,

(6) Protocol to reconstitute translationally arrested heat shock mRNPs and condensates in vitroSTAR ProtocolsSeptember 20, 2024Christine Desroches Altamirano, Simon Alberti#10621714001 Creatine phosphokinase,Sigma-Aldrich,Cat #C3755 Amino acid mixture (complete),Promega,Cat #L4461 Yeast tRNA,Thermo Fisher Scientific,Cat# AM7119 RNaseOUT,Invitrogen,Cat #10777019 Glycerol,Carl Roth,Cat

(7) Heat flows enrich prebiotic building blocks and enhance their reactivityNatureApril 4, 2024Thomas Matreux, Paula Aikkila, Bettina Scheu, Dieter Braun, Christof B. Mastwere purchased from Biolog (USA). For the proteogenic amino acids, an Amino Acid Mixture was used (L4461, Promega, USA) and compared with individual amino acids (Sigma-Aldrich, USA). For experiments on non-proteogenic

(8) N1-methylpseudouridylation of mRNA causes +1 ribosomal frameshiftingNatureJanuary 4, 2024Thomas E. Mulroney, Tuija Pöyry, Juan Carlos Yam-Puc, Maria Rust, Robert F. Harvey, Lajos Kalmar, Emily Horner, Lucy Booth, Alexander P. Ferreira, Mark Stoneley, et al.15 µl reaction. Unlabelled products were produced with 1 µl total (unlabelled) amino acids (Promega L4461). The concentration of IVT mRNA was 50 nM and, when included, the concentration of paromomycin (Sigma

(9) Reversible 2′-OH acylation enhances RNA stabilityNature ChemistrySeptember 1, 2023Linglan Fang, Lu Xiao, Yong Woong Jun, Yoshiyuki Onishi, Eric T. Kool0.5 μl of RNasin Plus RNase inhibitor (Promega, N2611), 2.0 μl of Complete Amino Acid mix (Promega, L4461) and 2.0 μl of 1 M KOAc. The resulting solution was mixed by gentle pipetting and then incubated at

(10) Nitric oxide-induced ribosome collision activates ribosomal surveillance mechanismsCell death & diseaseJuly 26, 2023Ryder L, Arendrup FS, Martínez JF, Snieckute G, Pecorari C, Shah RA, Lund AH, Blasius M, Bekker-Jensen S.(Sigma Aldrich, #10127566001), 10 µM spermidine (Sigma Aldrich, # S0266), 10 µM amino acids (Promega, # L4461), 65 mM KAc, 0.75 mM MgAc2 and murine RNase inhibitor A dual mRNA construct with a Renilla luciferase

(11) Human-rabbit Hybrid Translation System to Explore the Function of Modified RibosomesBio ProtocJuly 5, 2023Eriko Matsuura-Suzuki, Hirotaka Toh, Shintaro Iwasakicatalog number: 16229-95, stored at room temperature)Amino acid mixtures (Promega, catalog number: L4461, stored at -80 °C)Recombinant RNase inhibitor (TaKaRa, catalog number: 2313A, stored at -20 °C)Dual-Luciferase Reporter Assay System

(12) Human-rabbit Hybrid Translation System to Explore the Function of Modified RibosomesBIO-PROTOCOLJuly 5, 2023Eriko Matsuura-Suzuki, Hirotaka Toh, Shintaro Iwasakicatalog number: 16229-95, stored at room temperature)Amino acid mixtures (Promega, catalog number: L4461, stored at -80 °C)Recombinant RNase inhibitor (TaKaRa, catalog number: 2313A, stored at -20 °C)Dual-Luciferase Reporter Assay System

(13) Production of human translation-competent lysates using dual centrifugationRNA BiologyDecember 31, 2022Lukas-Adrian Gurzeler, Jana Ziegelmüller, Oliver Mühlemann, Evangelos D. Karousis0.68 mM MgCl2, 54.05 mM KCl, 0.05 mM amino acid mixture, unless otherwise stated (Promega, Cat. No. L4461), 13.51 mM Creatine Phosphate, 229.73 ng/ml Creatine kinase and 1x protease inhibitor cocktail (Bimake,

(14) Functional characterization of 5′ UTR cis-acting sequence elements that modulate translational efficiency in Plasmodium falciparum and humansMalaria JournalDecember 1, 2022Valentina E. Garcia, Rebekah Dial, Joseph L. DeRisi384-well plates. A master mix of 3.5 µL lysate with 0.5 µL 100 μm complete amino acid mix (Promega L4461) and 1uL 10x translation buffer (20 mM HEPES pH 8, 75 mM KoAc, 2 mM DTT, 5 mM ATP, 1 mM GTP 200 mM creatine

(15) PABP/purine-rich motif as an initiation module for cap-independent translation in pattern-triggered immunityCellAugust 18, 2022Wang J, Zhang X, Greene GH, Xu G, Dong X.#TER51020 Phos-tag (TM) Acrylamide,FUJIFILM Wako,Cat. #304–93521 "Amino Acid Mixture, Complete",Promega,Cat. #L4461 m7G(5′)ppp(5′)G RNA Cap Structure Analog,NEB,Cat. #S1404S SUPERase•In RNase Inhibitor,Invitrogen,Cat.

(16) NusG-mediated Coupling of Transcription and Translation Enhances Gene Expression by Suppressing RNA Polymerase BacktrackingJournal of Molecular BiologyJanuary 1, 2022Elizabeth J. Bailey, Max E. Gottesman, Ruben L. Gonzalezthe reaction; 5 μL of a solution that is 1 mM in each of the 20 essential amino acids (Promega, No. L4461); 20 μl of Promega S30 Premix without Amino Acids (No. L512A-C); vS30 μL of S30 cell extract, where

(17) Small Extracellular Vesicles Propagate the Inflammatory Response After TraumaAdvanced ScienceDecember 1, 2021Tanja Seibold et al.reticulocyte lysate system (Promega, #L4960) and non‐labeled complete amino acid mixture (Promega, #L4461) according to the manufacturer's description. After the translation reaction, samples were supplemented

(18) Development, validation, and application of the ribosome separation and reconstitution system for protein translation in vitroRNADecember 1, 2021Brandon M. Trainor, Dimitri G. Pestov, Natalia Shcherbiksolution of 20 essential amino acids, complete and minus Methionine and Cysteine, were from Promega (cat# L4461 and L5511, respectively); Ambion mMESSAGE mMACHINE T7 Transcription kit was purchased from Thermo Fisher Scientific

(19) 5' Half of specific tRNAs feeds back to promote corresponding tRNA gene transcription in vertebrate embryosScience advancesNovember 19, 2021Chen L, Xu W, Liu K, Jiang Z, Han Y, Jin H, Zhang L, Shen W, Jia S, Sun Q, et al.reaction was assembled as follows: 9.9 μl of RRL, 0.3 μl of 1 mM complete amino acid mixture (Promega, L4461), 0.42 μl of 3 M KCl, 0.39 μl of RNase inhibitor, 1 μl of GFP mRNA (500 ng/μl), 0.5 μl of purified NLS-2xFlag-mCherry-HisTag

(20) The eIF2 kinase GCN2 directs keratinocyte collective cell migration during wound healing via coordination of reactive oxygen species and amino acidsThe Journal of biological chemistryNovember 1, 2021Miles RR, Amin PH, Diaz MB, Misra J, Aukerman E, Das A, Ghosh N, Guith T, Knierman MD, Roy S, et al.of 1000 V/cm. A calibration curve was prepared using a sample containing all 20 amino acids (Promega L4461). The amino acid standard was diluted 1:100 to make 10 μM standard in BGE metabolite diluent provided

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