AlwNI from New England Biolabs

Product Specs
ItemAlwNI
CompanyNew England Biolabs
Price $308.00 Inquire
Catalog NumberR0514L
Concentration10 u/µL
Quantity2500 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

CAGNNN/CTG

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Profile of GAS6 gene single nucleotide polymorphisms [GAS6 c.834+7G>A (rs8191974), and GAS6 c.1332C>T (rs1803628)] among a cohort of Egyptian women with preeclampsia: Relation to disease risk and severityJournal of Genetic Engineering and BiotechnologyDecember 1, 2025Mostafa Mohamed Khodry, Sawsan M.A. Abuhamdah, Mohammed H. Hassan, Abd El-Naser Abd El-Gaber Ali, Asmaa Ahmed Sayed Khames, Mohammad A.M. Ahmed(Fig. 1). The PCR amplification products were digested using restriction enzymes. AlwN1 (catalog No. R0514S, supplied by New England Biolabs) was used for the detection of GAS6 c.834 + 7G > A, and NlaIII (catalog

(2) RNA polymerase II is a polar roadblock to a progressing DNA forkNature CommunicationsSeptember 29, 2025Taryn M. Kay, James T. Inman, Lucyna Lubkowska, Tung T. Le, Jin Qian, Porter M. Hall, Sahil Batra, Dirk Remus, Dong Wang, Mikhail Kashlev, et al.the same region of pRL574 as the CD template but digested with both BstXI (NEB R0113) and AlwNI (NEB R0514S), generating a 1.5 kb strand. The upstream segment was ligated to the upstream end of the elongation

(3) Association of macrophage migration-inhibitory factor gene and growth differentiation factor 15 gene polymorphisms and their circulating levels with respiratory distress syndrome among preterm neonatesClinical and Experimental PediatricsSeptember 15, 2025Ali Helmi Bakri, Mohammed H. Hassan, Khaled Abdalla Abd-Elbaseer, Mahmoud Abo-Alhassan Sayed, Ahmed Alamir Mahmoud Abdallah, Eman Ahmed Abd-Elmawgoodusing a restriction enzyme (AlwNI), which was provided by New England Biolabs and has catalogue number R0514S. The final digestion products were separated on 2.0% agarose gels, then stained with ethidium bromide

(4) Control of DNA replication in vitro using a reversible replication barrierNature ProtocolsJuly 1, 2024Emma J. Vontalge, Tamar Kavlashvili, Steven N. Dahmen, Matthew T. Cranford, James M. DewarD8418) Glycogen (Roche, cat. no. 10901393001) XmnI (NEB, cat. no. R0194S) AlwNI (NEB, cat. no. R0514S) Trichloroacetic acid (TCA) (Thermo Fisher Scientific, cat. no. FLA322500) XhoI (NEB, cat. no. R0146S)

(5) Functional analysis of germline VANGL2 variants using rescue assays of vangl2 knockout zebrafishHuman Molecular GeneticsJanuary 7, 2024Christopher J Derrick, Emmanuelle Szenker-Ravi, Adrian Santos-Ledo, Ahlam Alqahtani, Amirah Yusof, Lorraine Eley, Alistair H L Coleman, Sumanty Tohari, Alvin Yu-Jin Ng, Byrappa Venkatesh, et al.5′-CAGCCTTTATACTCTCTTCCATTGG-3′ and Reverse 5′-CAGAAATGCCTGACCACAGATTC-3′ and digestion with AlwNI (NEB, R0514) for 3.5 h. The reverse primer (base change for dCAPs underlined) together with the m209 mutation (3′

(6) Etoposide promotes DNA loop trapping and barrier formation by topoisomerase IINature Chemical BiologyMay 1, 2023Tung T. Le, Meiling Wu, Joyce H. Lee, Neti Bhatt, James T. Inman, James M. Berger, Michelle D. WangGTAGCACCAAAGGAAACCATCACCCA with Phusion DNA polymerase (NEB, M0530S). The PCR product was digested with AlwNI (NEB, R0514S), gel purified and ligated to the 2-kb DNA Y-arms40 using T4 Ligase (NEB, M0202S). Single-molecule

(7) Polarity of the CRISPR roadblock to transcriptionNature Structural & Molecular BiologyDecember 5, 2022Porter M. Hall, James T. Inman, Robert M. Fulbright, Tung T. Le, Joshua J. Brewer, Guillaume Lambert, Seth A. Darst, Michelle D. Wangtranscription complex (PTC) mapping, and generated by PCR and digested with AlwNI (NEB, catalog no. R0514; Supplementary Table 4). These DNA segments were then each ligated to a pair of dsDNA arms containing

(8) Bi-allelic MCM10 variants associated with immune dysfunction and cardiomyopathy cause telomere shorteningNature communicationsMarch 12, 2021Baxley RM, Leung W, Schmit MM, Matson JP, Yin L, Oram MK, Wang L, Taylor J, Hedberg J, Rogers CB, et al.(MCM10 exon 3, Hpy199III (NEB R0622); CDC45 exon 3, PflMI (NEB R0509), XcmI (NEB R0533) or AlwNI (NEB R0514); MCM4 exon 2, BglI (NEB R0143). To generate HCT116 MUS81-/- exon 2 mutant cell lines, each parental

(9) A Well-Mixed E. coli Genome: Widespread Contacts Revealed by Tracking Mu TranspositionCellFebruary 13, 2020David M. Walker, Peter L. Freddolino, Rasika M. Harshey(TdT),New England Biolabs,M0315S HinP1,HinP1,New England Biolabs,R0124S AlwNI,AlwNI,New England Biolabs,R0514S BglII,BglII,New England Biolabs,R0144S T4 DNA ligase,T4 DNA ligase,New England Biolabs,M0202S Phusion

(10) Topoisomerase II Is Crucial for Fork Convergence during Vertebrate Replication TerminationCell ReportsOctober 8, 2019Darren R. Heintzman, Lillian V. Campos, Jo Ann W. Byl, Neil Osheroff, James M. DewarM280 Streptavidin,Invitrogen,11205D hTop2a (Topogen),Topogen,TG2000H-1 AlwNI,New England Biolabs,R0514 XmnI,New England Biolabs,R0194 Nt.BspQI,New England Biolabs,R0644 Nb.BssSI,New England Biolabs,R0681 Biotin,Sigma,B4501 T4 Polynucleotide Kinase

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AlwNI

AlwNI from New England Biolabs

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New England Biolabs

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AlwNI from New England Biolabs

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