AlwI from New England Biolabs

Product Specs
ItemAlwI
CompanyNew England Biolabs
Price $75.00 Inquire
Catalog NumberR0513S
Quantity500 units
ApplicationsDNA Manipulation
Concentration2 u/µL
TypeRestriction Enzyme
Temperature-20 C

Sequence

GGATC(4/5)

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Pre-marking chromatin with H3K4 methylation is required for accurate zygotic genome activation and developmentNat CommunDecember 19, 2025Meghana S. Oak, Marco Stock, Ana Janeva, Matthias Mezes, Antony M. Hynes-Allen, Tobias Straub, Ignasi Forné, Andreas Ettinger, Stephan Hamperl, Axel Imhof, et al.column-purified with the QIAquick PCR Purification Kit (Qiagen, 28104), sonicated and digested with AlwI (NEB, R0513S) before sequencing library preparation. CATaDa fragments were prepared for Illumina sequencing according

(2) Multiscale characterization of Caenorhabditis elegans mutants to probe functional mechanisms of human actin pathological variantsiScienceNovember 21, 2025Théo Hecquet, Nadine Arbogast, Delphine Suhner, Anaïs Goetz, Grégory Amann, Selin Yürekli, Fiona Marangoni, Sophie Quintin, Johannes N. Greve, Nataliya Di Donato, et al.,New England Biolabs,R018L Restriction enzyme: BglII,New England Biolabs,R0144L Restriction enzyme: AlwI,New England Biolabs,R0513S Restriction enzyme: MspA1I,New England Biolabs,R0577S Cas9,homemade,– F-127,Sigma-Aldrich,P2443;

(3) SMCHD1 maintains heterochromatin, genome compartments and epigenome landscape in human myoblastsNature communicationsJuly 26, 2025Huang Z, Cui W, Ratnayake I, Gallik KL, Cohen L, Tawil R, Pfeifer GP.sonicator to shear the DNA to 300 to 500 bp size fragments. We incubated 1 μL of AlwI enzyme (NEB, R0513S) and sheared mixtures at 37 °C for overnight to remove the DamID adaptors. We cleaned up the mixtures

(4) Lateral Flow Biosensor for On-Site Multiplex Detection of Viruses Based on One-Step Reverse Transcription and Strand Displacement AmplificationBiosensors (Basel)February 17, 2024Xuewen Lu, Kangning Ding, Zhiyuan Fang, Yilei Liu, Tianxing Ji, Jian Sun, Zhenling Zeng, Limin HeBiological Engineering Technology (Shanghai, China). Klenow Fragment (3′→5′ exo-) (M0212) and Nt.AlwI (R0513) were purchased from New England biolabs (Ipswich, MA, USA). HAuCl4 was purchased from Sigma-Aldrich

(5) Notch signaling and Bsh homeodomain activity are integrated to diversify Drosophila lamina neuron typeseLifeJanuary 9, 2024Chundi Xu, Tyler B Ramos, Owen J Marshall, Chris Q Doecompound, drug",MyTaq HS DNA Polymerase,Bioline,Cat#BIO-21112, "Chemical compound, drug",AlwI,NEB,Cat#R0513S, "Chemical compound, drug",RNase A (DNase free),Roche,Cat#11119915001, "Chemical compound, drug",T4 DNA ligase

(6) Notch signaling and Bsh homeodomain activity are integrated to diversify Drosophila lamina neuron typesElifeJanuary 9, 2024Chundi Xu, Tyler B Ramos, Owen J Marshall, Chris Q Doecompound, drug",MyTaq HS DNA Polymerase,Bioline,Cat#BIO-21112, "Chemical compound, drug",AlwI,NEB,Cat#R0513S, "Chemical compound, drug",RNase A (DNase free),Roche,Cat#11119915001, "Chemical compound, drug",T4 DNA ligase

(7) Homeodomain proteins hierarchically specify neuronal diversity and synaptic connectivityeLifeJanuary 5, 2024Chundi Xu, Tyler B Ramos, Edward M Rogers, Michael B Reiser, Chris Q Doecompound, drug",MyTaq HS DNA Polymerase,Bioline,Cat#BIO-21112, "Chemical compound, drug",AlwI,NEB,Cat#R0513S, "Chemical compound, drug",RNase A (DNase free),Roche,Cat#11119915001, "Chemical compound, drug",T4 DNA ligase

(8) Homeodomain proteins hierarchically specify neuronal diversity and synaptic connectivityElifeJanuary 5, 2024Chundi Xu, Tyler B Ramos, Edward M Rogers, Michael B Reiser, Chris Q Doecompound, drug",MyTaq HS DNA Polymerase,Bioline,Cat#BIO-21112, "Chemical compound, drug",AlwI,NEB,Cat#R0513S, "Chemical compound, drug",RNase A (DNase free),Roche,Cat#11119915001, "Chemical compound, drug",T4 DNA ligase

(9) iMUT-seq: high-resolution DSB-induced mutation profiling reveals prevalent homologous-recombination dependent mutagenesisNat CommunDecember 18, 2023Aldo S. Bader, Martin Bushellamplicon was then digested with the restriction enzymes Afl-II, Bts-I and Alw-I (NEB, #R0520, #R0667 and #R0513 respectively) for 4 hours at 37 C with 300 rpm agitation in 1x CutSmart buffer (NEB). Following this,

(10) In vivo, genome-wide profiling of endogenously tagged chromatin-binding proteins with spatial and temporal resolution using NanoDam in DrosophilaSTAR ProtocDecember 16, 2022Jocelyn L.Y. Tang, Robert Krautz, Oriol LlorÃ -Batlle, Anna E. Hakes, Paul M. Fox, Andrea H. Brandrecombinant proteins" ,, 10 mM Tris-HCl (pH 8.0),N/A,N/A 100% ethanol,VWR,Cat#20821.330 AlwI,NEB,Cat#R0513L Autoclaved dH2O,N/A,N/A "Cytiva Sera-mag Speedbeads (Carboxyl magnetic beads, 1 μM, 15 mL)",Fisher

(11) A Notch-dependent transcriptional mechanism controls expression of temporal patterning factors in Drosophila medullaeLifeAugust 30, 2022Alokananda Ray, Xin Li‘DamID-seq’ in ‘Materials and methods’ "Peptide, recombinant protein",AlwI,New England Biolabs,Catalog_#:R0513S,See section ‘DamID-seq’ in ‘Materials and methods’ "Peptide, recombinant protein",Quick Ligation kit,New England Biolabs

(12) A Notch-dependent transcriptional mechanism controls expression of temporal patterning factors in Drosophila medullaElifeAugust 30, 2022Alokananda Ray, Xin Li‘DamID-seq’ in ‘Materials and methods’ "Peptide, recombinant protein",AlwI,New England Biolabs,Catalog_#:R0513S,See section ‘DamID-seq’ in ‘Materials and methods’ "Peptide, recombinant protein",Quick Ligation kit,New England Biolabs

(13) BMAL1 moonlighting as a gatekeeper for LINE1 repression and cellular senescence in primatesNucleic acids researchApril 8, 2022Liang C, Ke Q, Liu Z, Ren J, Zhang W, Hu J, Wang Z, Chen H, Xia K, Lai X, et al.previously described (84). For adaptor trimming, the amplified DNA was sonicated and digested with AlwI (R0513S, New England Biolabs) enzyme. The DNA library was constructed using a NEBNext® Ultra™ DNA library prep

(14) Microbes affect gut epithelial cell composition through immune-dependent regulation of intestinal stem cell differentiationCell reportsMarch 29, 2022Liu X, Nagy P, Bonfini A, Houtz P, Bing XL, Yang X, Buchon N.E220 sonicator (Covaris microtube 520045). DamID adaptors were removed by AlwI (New England Biolabs R0513S) overnight digestion. AMPure XP magnetic beads (Beckman Coulter A63880) were used for cleanup for sequencing

(15) In vivo targeted DamID identifies CHD8 genomic targets in fetal mouse brainiScienceNovember 19, 2021A. Ayanna Wade, Jelle van den Ameele, Seth W. Cheetham, Rebecca Yakob, Andrea H. Brand, Alex S. Nordwere sonicated and prepped for sequencing. Sonicated samples were subjected to AlwI digestion (NEB, R0513S) to remove previously ligated adaptors and initial GATC sequences from fragments. A modified TruSeq

(16) Stem cell niche organization in the Drosophila ovary requires the ECM component PerlecanCurrent BiologyApril 26, 2021Alfonsa DÃaz-Torres, Alicia E. Rosales-Nieves, John R. Pearson, Carmen Santa-Cruz Mateos, Miriam MarÃn-Menguiano, Owen J. Marshall, Andrea H. Brand, Acaimo GonzÃ¡lez-ReyesT2694 DpnII and DpnII buffer,NEB,Cat# R0543S Advantage 2 cDNA polymerase,Clontech,Cat# 639201 AlwI,NEB,Cat# R0513S RNase A (DNase free),Roche,Cat# 11119915001 Qubit assay tubes,Invitrogen,Cat# Q32856 Qubit dsDNA HS assay kit

(17) A KDM5–Prospero transcriptional axis functions during early neurodevelopment to regulate mushroom body formationeLifeMarch 17, 2021Hayden AM Hatch, Helen M Belalcazar, Owen J Marshall, Julie SecombeNew England BioLabs Cat# R0176 Other DpnI I New England BioLabs Cat# R0543 Other AlwI New England BioLabs Cat# R0513 Hatch et al. eLife 2021;10:e63886. DOI: https://doi.org/10.7554/eLife.63886 Additional information 29

(18) Stabilization of heterochromatin by CLOCK promotes stem cell rejuvenation and cartilage regenerationCell researchFebruary 1, 2021Liang C, Liu Z, Song M, Li W, Wu Z, Wang Z, Wang Q, Wang S, Yan K, Sun L, et al.previously described.58 For adapter trimming, the amplified DNA was sonicated and digested with AlwI (R0513S, New England Biolabs). The DNA library was constructed using a NEBNext Ultra DNA Library Prep Kit for

(19) Ets21c Governs Tissue Renewal, Stress Tolerance, and Aging in the Drosophila IntestineCell ReportsJune 4, 2019Juliane Mundorf, Colin D. Donohoe, Colin D. McClure, Tony D. Southall, Mirka Uhlirova,New England Biolabs,Cat. #R0543 MyTaq HS DNA Polymerase,Bioline,Cat. #BIO-21112 AlwI,New England Biolabs,Cat. #R0513 Phusion High-Fidelity DNA Polymerase,New England Biolabs,Cat. #M0530 Paraquat,Sigma-Aldrich,Cat. #856177 "1,4-Diazabicyclo[2.2.2]octane

(20) Stem Cell Proliferation Is Kept in Check by the Chromatin Regulators Kismet/CHD7/CHD8 and Trr/MLL3/4Developmental CellMay 20, 2019Louis Gervais et al.B0202S DpnI,NEB,Cat#R0176L DpnII,NEB,Cat#R0543L Sau3AI,NEB,Cat# R0169L PCR buffer MyTaq HS,Bioline,Cat# BIO-21111 AlwI,NEB,Cat# R0513S Klenow fragment,NEB,Cat# 210S T4 polynucleotide kinase,NEB,Cat# M0201S Elastase,Sigma,Cat# E0258-5MG ,, Critical

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AlwI

AlwI from New England Biolabs

Sequence

New England Biolabs

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Reviews

AlwI from New England Biolabs

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