AluI from New England Biolabs

Product Specs
ItemAluI
CompanyNew England Biolabs
Price $75.00 Inquire
Catalog NumberR0137S
Quantity1000 units
Concentration8 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

AG/CT

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Highly efficient chromatin conformation capture with post-enrichment in single cells by HiChewGenome BiolApril 27, 2026Zhichao Chen, Yeming Xie, Chen Zhang, Chen Tan, Fengying Ruan, Wenfang Chen, Meng Luo, Mei Guo, Zhe Xie, Huimin Cao, et al.and ligation For restriction digestion, 200 U of DpnII (NEB, Cat. No. R0543L) or AluI (NEB, Cat. No. R0137L) was added to the reaction and incubated at 37 °C overnight. Aliquots were de-crosslinked with proteinase

(2) Chromosome centromere copy number amplification associated with exceptional response in HER2-positive metastatic breast cancer patientsOncogeneFebruary 10, 2026Charlotte Andrieu, Jojanneke Stoof, Dalal AlSultan, Laura Ivers, Jose Javier Berenguer Pina, Darko Skrobo, Jo Ballot, Debbie O’Reilly, Denis M. Collins, Alex J. Eustace, et al.quantified by Nanodrop. DNA was AluI digested through 15-min incubation at 37 °C with AluI enzyme (#R0137S, New England Biolabs, Ipswich, MA USA), followed by 20-min incubation at 80 °C to inactivate the enzyme.

(3) Topologically Associating Domains Define the 3D Genome Architecture of Mouse Totipotent-like Stem CellsJournal of Biological ChemistryFebruary 5, 2026Yu Fu, Wenyu Ma, Jin Han, Wenhui Zhang, Shenghu Zhang, Yuxiao Chen, Yue Qin, Junyang Li, Ziwei Zhai, Pengli Li, et al.at room temperature, then quenched with 3% Triton X-100. The chromatin was digested with AluI (NEB, R0137L) and HpyCH4V (NEB, R0620L) at 37 °C overnight. A-tailing of the digestion ends was achieved by dATP

(4) Inverse Restriction Site-Associated DNA Sequencing (iRAD-seq)BIO-PROTOCOLJanuary 20, 2026Peng Chen, Shen Zhou, Haonan Wang, Jiaqi Gu, Yongquan Li, Yuxiao Chang(New England Biolabs, catalog number: R0106S) 15. AluI 10 U/μL (New England Biolabs, catalog number: R0137S) 16. 2% agarose (Sage Science, catalog number: HTC2010, 100–600 bp) Equipment 1. Qubit fluorometer

(5) Systematic enhancer mapping and functional analysis in zebrafish with optimized CRISPR interferenceNucleic Acids ResDecember 11, 2025Jiulin Chan, Zhichao Wu, Mingli Liu, Tianming Wang, Hanyu Liu, Ruimeng Cao, Xiaolong Li, Xinwen Li, Siyao Zhan, Jiangbo Cheng, et al.containing rCutSmart™ Buffer (NEB, Cat#B6004V) and 400 units of HaeIII and AluI (NEB, Cat#R0108S and Cat#R0137) and incubating overnight at 37°C. A-tailing, proximity ligation, and linker removal Digestion mixture

(6) Dynamic control of autoreactive B cells from spontaneous germinal centersThe Journal of ImmunologyDecember 1, 2025Kristine Oleinika, Alexia Correia Correia Ferreira, Selma Mouftakir, Carlos Castrillon, Lisa Madungwe, Usha Nair, Facundo D. Batista, Michael C. Carrollwere genotyped using droplet digital PCR (ddPCR). DNA was digested with Alu I (New England Biolabs, R0137S), droplets generated with DNA, primers and EvaGreen Supermix (Bio-Rad, 1864034) using a QX200 Droplet Generator

(7) Rbm8a deficiency causes hematopoietic defects by modulating Wnt/PCP signalingDevelopmental BiologyDecember 1, 2025Agnese Kocere, Elena Chiavacci, Charlotte Soneson, Seth T. Jacobson, Emma N. Harrison, Kevin Manuel Méndez-Acevedo, Jacalyn S. MacGowan, Harrison H. Wells, Max S. Hiltabidle, Azhwar Raghunath, et al.primer vangl2 rev 5′-AGCGCGTCCACCAGCGACACAGC-3′ or restriction digest of the PCR products with Alu1 (R0137S, NEB). The vangl2 wild type allele stayed intact while the vangl2vu67 mutant allele was identified by

(8) Deciphering the epigenomic regulatory variations reveals function diversity in adipose lineage among different adipose depots of pigsCell & BioscienceNovember 26, 2025Daoyuan Wang, Renzhuo Kuang, Mingyang Hu, Jiahao Sun, Zhixiang Xu, Yu Shen, Hao Peng, Xiaolong Qi, Honghong Zhou, Yaping Guo, et al.with 1% FA (F8775, Sigma) and 2.5 mM EGS (21,565, Thermo Scientific), and restriction enzymes AluI (R0137, NEB) and HaeIII (R0108, NEB) were used to digest chromatin, and biotin-labled bridge linker were used

(9) Integrating Hi-C data and RNA-seq data to reveal the 3D genome in sheepBMC GenomicsNovember 17, 2025Quanzhong Xu, Xiaoxue Zhang, Fadi Li, Huibin Tian, Deyin Zhang, Kai Huang, Yukun Zhang, Yuan Zhao, Xiaolong Li, Liming Zhao, et al.lysis process. The chromatin was then digested with the AluI restriction enzyme (NEB, Catalog No.: R0137L) at 37 °C for 7 h. Following digestion, the samples were incubated with dATP (NEB, Catalog No.: N0440S)

(10) Mapping the Telomeric 3D Interactome with Telomere-C Reveals Repetitive Element Hubs Associated with Telomere MaintenancebioRxivOctober 14, 2025Yi-An Chen, Ogechukwu Mbegbu, Noelle H. Fukushima, T. Rhyker Ranallo-Benavidez, Tianpeng Zhang, Floris P. BarthelSDS and incubated for 10 minutes at 65°C followed by 10% Triton X-100 for quenching. AluI (520U, NEB, R0137L) was added, incubated overnight at 37°C, and then inactivated by incubating for 20 minutes at 65°C.

(11) Genetics-nutrition interactions control diurnal enhancer-promoter dynamics and liver lipid metabolismCell MetabolismOctober 7, 2025Dishu Zhou, Ying Chen, Panpan Liu, Kun Zhu, Juliet Holder-Haynes, S. Julie-Ann Lloyd, Cam Mong La, Inna I. Astapova, Seunghee Choa, Ying Xiong, et al.TRIzol Reagent,Ambion,5457 SYBR Green PCR Master Mix,ThermoFisher Scientific,4334973 SPRIselect,Beckman coulter,B23318 AluI,NEB,R0137L Dynabeads™ M-280 Streptavidin,Thermo Fisher,11206D IGEPAL CA-630,Sigma,I8896 DynaBeads Protein G,Thermo Fisher

(12) Small-scale in situ Hi-C protocol for early embryos to resolve the three-dimensional genome structureSTAR ProtocolsSeptember 19, 2025Linjie Song, Jingcheng Zhang, Linqian Pan, Shengjie Sun, Binqiang Shi, Hexu Zhang, Hengkuan Li, Zixiao Liu, Linmi Li, Yong Zhang, et al.K,Roche,Cat#3115887001 Phenol:chloroform:isoamyl alcohol mixture,Solarbio,Cat#P1012 Sodium acetate,Thermo Scientific,Cat#R1181 Alu I,NEB,Cat#R0137S rCutSmart Buffer,NEB,Cat#B6004V EDTA,Beyotime,Cat#ST066 Tris-HCl (pH 7.5),Beyotime,Cat#ST775 Elution

(13) The shibire ts4 mutation causes temperature sensitive paralytic and lethal phenotypes in the Queensland fruit fly, Bactrocera tryoniInsect ScienceAugust 29, 2025Anzu Okada, Mamoru Okamoto, Thu N.M. Nguyen, Elisabeth Fung, Han Nguyen, Peter Crisp, Amanda Choo, Simon W. Baxtermutation. The amplicons were digested with the AluI restriction enzyme (New England Biolabs, Cat. No. R0137L) at 37 °C for 1 h and gel electrophoresis was performed using 3% agarose gel to distinguish potential

(14) Comprehensive Genetic Map of Muscle Lipidome Reveals Novel Insights Into Flavor Variation in Ruminant MeatAdvanced ScienceAugust 11, 2025Xueying Zhang, Yuanyuan Kong, Jialei Chen, Ran Li, Zhongyu Wang, Yali Song, Tianshu Dai, Yuxin Fu, Beixiang Jiang, Jize Zeng, et al.with Triton X‐100 (Thermo Scientific, 28 314). Chromatin was digested with AluI endonuclease (NEB, R0137L) at 37 °C for 7 h, followed by labeling of the DNA fragment ends with biotin (0.4 mm biotin‐14‐dATP

(15) Selfish mutations promote age-associated erosion of mtDNA integrity in mammalsNat CommunJuly 1, 2025Ekaterina Korotkevich, Daniel N. Conrad, Zev J. Gartner, Patrick H. O'Farrellforward primer, 250 nM of reverse primer, 250 nM of probe, 0.5 μL of restriction enzyme [AluI (NEB, R0137L) for mouse assay and HaeIII (NEB, R0108L) for human assay]) was added to each of 96 wells (final volume

(16) A suite of selective pressures supports the maintenance of alleles of a Drosophila immune peptideElifeMay 30, 2025Sarah R Mullinax, Andrea M Darby, Anjali Gupta, Patrick Chan, Brittny R Smith, Robert L Uncklessdigest using enzymes that cut DNA sequences for arginine (BccI, NEB # R0704S) or serine (AluI, NEB # R0137S). DNA concentration was measured with Qubit fluorimeter (Invitrogen). Ten flies from each inbred CRISPR

(17) Control of replication and gene expression by ADP-ribosylation of DNA in Mycobacterium tuberculosisThe EMBO JournalMay 8, 2025Rachel E Butler, Marion Schuller, Ritu Jaiswal, Jayanta Mukhopadhyay, Jim Barber, Suzie Hingley-Wilson, Emily Wasson, Alexassander Couto Alves, Ivan Ahel, Graham R Stewartisoamyl alcohol mixture,Merck,77617 QuantiFluor® dsDNA System,Promega,E2671 Alu1,New England Biolabs,R0137 Mse1,New England Biolabs,R0525 Sal1,New England Biolabs,R0138 Zeta-Probe® Membrane,Bio-Rad,#1620158 Clarity

(18) Tri-omic single-cell mapping of the 3D epigenome and transcriptome in whole mouse brains throughout the lifespanNature MethodsMay 1, 2025Haoxi Chai, Xingyu Huang, Guangzhou Xiong, Jiaxiang Huang, Katarzyna Karolina Pels, Lingyun Meng, Jin Han, Dongmei Tang, Guanjing Pan, Liang Deng, et al.Triton X-100 and incubated at 37° C for 20 min. Cells/nuclei were then in situ digested by AluI (NEB; R0137L) or AluI+HpyCH4V (NEB; R0620L) restriction enzymes at 37 °C for at least 2 h or overnight by adding

(19) Mitochondrial metabolism sustains DNMT3A-R882-mutant clonal haematopoiesisNatureApril 16, 2025Malgorzata Gozdecka, Monika Dudek, Sean Wen, Muxin Gu, Richard J. Stopforth, Justyna Rak, Aristi Damaskou, Guinevere L. Grice, Matthew A. McLoughlin, Laura Bond, et al.thus PCR products were subsequently digested with the restriction enzyme AluI (New England Biolabs, R0137S) for 1 h at 37 °C and visualized on 2.5% agarose gel. Lentiviral-vector production and transduction Lentiviruses

(20) Evolutionary divergence in CTCF-mediated chromatin topology drives transcriptional innovation in humansNat CommunMarch 26, 2025Xia Wu, Dan Xiong, Rong Liu, Xingqiang Lai, Yuhan Tian, Ziying Xie, Li Chen, Lanqi Hu, Jingjing Duan, Xinyu Gao, et al.method. Subsequently, ~2 million dual-crosslinked cells underwent AluI (New England Biolabs, Cat. No. R0137) digestion, A-tailing, proximal ligation to biotinylated linkers, and sonication, following the corresponding

Be the First to Write a Review!

AluI

AluI from New England Biolabs

Sequence

New England Biolabs

Citations (90)

Reviews

AluI from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map